OPTN- Recipient Histocompatibility

Recipient Histocompatibility (RHS) Record Field
Descriptions
Recipient Histocompatibility (RHS) records are generated as soon as the Recipient Feedback is
completed in Tiedi®. For living donors, the RHS record is generated when Living Donor Feedback has
been completed in Tiedi. The RHS record is to be completed by the histocompatibility laboratory
responsible for performing tissue typing on the recipient and crossmatch testing between the donor and
recipient once an organ becomes available.
The RHS record must be completed within 30 days from the record generation date. See OPTN/UNOS
Policies for additional information. Use the search feature to locate specific policy information on Data
Submission Requirements.
To correct information that is already displayed in an electronic record, call the UNOS Help Desk at 1800-978-4334.
Provider Information
Lab: The lab, from Donor Organ Disposition in DonorNet, displays.
TX Center: The center, from Recipient Feedback, displays.
Recipient Information
Name: The recipient's name, from Recipient Feedback, displays.
DOB: The recipient's date of birth, from Recipient Feedback, displays.
Transplant Date: The recipient’s transplant date, from Recipient Feedback, displays.
Note: The transplant date is the date of the beginning of the first anastomosis. If the operation
started in the evening and the first anastomosis began early the next morning, the
transplant date is the date that the first anastomosis began. The transplant is considered
complete when the cavity is closed and the final skin stitch/staple is applied.
SSN: The recipient's social security number, from Recipient Feedback, displays.
Gender: The recipient's gender, from Recipient Feedback, displays.
HIC: The recipient's Health Insurance Claim (HIC) number, from Recipient Feedback, displays.
Organ(s): The recipient’s transplanted organ(s), from Recipient Feedback, displays.
Donor Information
UNOS Donor ID #: The donor's identification number, from Recipient Feedback, displays.
Donor Type: The donor type, from Recipient Feedback, displays.
Deceased indicates the donor was not living at the time of donation.
Living indicates the donor was living at the time of donation.
Test Information
HLA typing Done: If HLA typing was performed on this recipient, select Yes. If HLA typing was not
performed, select No. If it is unknown if the recipient was typed, select UNK. This field is required. If
HLA typing was done on the recipient, complete Section I.

HLA Antibody Screening Done: If HLA antibody screening was done on the recipient, select Yes. If
not, select No. This field is required. If screening was done on the recipient, complete Section II.
Crossmatch Done: If crossmatch testing was done on the recipient, select Yes. If not, select No. This
field is required. If crossmatch testing was done on the recipient, complete Section III.
If yes, was the crossmatch prospective to transplant: If the crossmatch was prospective, select
Yes. If not, select No. If unknown, select UNK. If Yes is selected for Crossmatch Done, this field is
required.
Donor Retyped at Your Center: If the donor was retyped at your laboratory, select Yes. If not, select
No. This field is required. If the donor was retyped at your laboratory, complete Section IV.
Section I - Recipient HLA Typing
Note: If Yes is selected for HLA typing Done, the fields in Section I are required.
Date Typing Complete Class I: Enter the date that the recipient was tissue-typed by the
histocompatibility laboratory for Class I antigens. Use the standard 8-digit numeric format of
MM/DD/YYYY.
Typing Method Class I: Select, as appropriate, the typing method. (List of Typing Method
codes)
Serology
DNA
Serology and DNA
Select the antigens from the drop-down lists. If the second antigen at a locus is blank, select No
second antigen detected. If the donor was not tested for a particular antigen, select Not tested.

Note: If the split of an antigen is known, enter the split rather than the parent.
A Locus Codes: (List of A Locus codes) 1, 2, 3, 9, 10, 11, 19, 23, 24, 25, 26, 28, 29, 30, 31,
32, 33, 34, 36, 43, 66, 68, 69, 74, 80, 203, 210, 2403, 6601, 6602, Unknown, No second
antigen detected, Not Tested
B Locus Codes: (List of B Locus codes) 5, 7, 8, 12, 13, 14, 15, 16, 17, 18, 21, 22, 27, 35, 37,
38, 39, 40, 41, 42, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63,
64, 65, 67, 70, 71, 72, 73, 75, 76, 77, 78, 81, 703, 804, 1304, 2708, 3901, 3902, 3905, 4005,
5102, 5103, 8201, Unknown, No second antigen detected, Not Tested

Bw4 HLA Codes: (List of Workgroup HLA codes) Positive, Negative, Not Tested
Bw6 HLA Codes: (List of Workgroup HLA codes) Positive, Negative, Not Tested
Cw HLA Codes: (List of Cw HLA codes) 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 13, 14, 15, 16,
17, 18, No second antigen detected, Not tested, No antigen detected
Date Typing Complete Class II: Enter the date that the recipient was tissue typed by the
histocompatibility laboratory for Class I antigens. Use the standard 8-digit numeric format of
MM/DD/YYYY. This field is required.
Typing Method Class II: Select, as appropriate, the typing method(s). This field is required. (List
of Typing Method codes)
Serology
DNA

Select the antigens from the drop-down lists. If the second antigen at a locus is blank, select No
second antigen detected. If the donor was not tested for a particular antigen, select Not tested.
These fields are required.

Note: If the split of an antigen is known, enter the split rather than the parent.
DR Locus Codes: (List of DR Locus codes) 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14,
15, 16, 17, 18, 103, 1403, 1404, No second antigen detected, Not tested
DR51 HLA Code: (List of Workgroup HLA codes) Positive, Negative, Not Tested
DR52 HLA Code: (List of Workgroup HLA codes) Positive, Negative, Not Tested
DR53 HLA Code: (List of Workgroup HLA codes) Positive, Negative, Not Tested
DQ HLA Codes: (List of DQ HLA codes) 1, 2, 3, 4, 5, 6, 7, 8, 9, No second antigen detected,
Not tested
DPW HLA Codes: (List of DP HLA codes) 1, 2, 3, 4, 5, 6, No second antigen detected, Not
tested
Note: Not tested is not a valid option for the DR locus codes for kidney, pancreas or
kidney/pancreas recipients on a kidney record.
Section II - HLA Antibody Screening
Note: If Yes is selected for HLA Antibody Screening Done, the fields in Section II are required.
A. Most Recent Complete the most recent anti-HLA Class I antibodies and PRA data for the Class I
section. If the serum was screened for anti-HLA Class II antibodies then complete the Class II section.
Serum Date - Most Recent Class I: Enter the date the serum was most recently collected and tested
to obtain Class I antigen results before the transplant was performed. Use the standard 8-digit
numeric format of MM/DD/YYYY. This field is required. If date is unavailable, select the reason from
the status (ST) drop-down list (N/A, Not Done, Missing, Unknown). (List of Status codes)
Target - Most Recent Class I: Select the target from the drop-down list. This field is required. (List
of PRA Target codes)

Cells
Purified HLA antigens, pooled
Purified HLA antigens from individual phenotypes
Purified single HLA antigens
Technique - Most Recent Class I: Select the technique from the drop-down list. This field is
required. If Other, specify is selected, enter the technique in the Specify field. If Other, specify is
selected, the Specify field is required. (List of PRA Technique codes)
Cytotoxity testing - extended incubation
Cytotoxity testing - wash
Cytotoxity testing - wash and extended incubation
Cytotoxity testing - AHG
Flow cytometry with cell targets
Flow cytometry with bead targets

ELISA
Other, specify
Technique Measures - Most Recent Class I: Select the technique from the drop-down list. This field
is required. (List of PRA Measure codes)
IgG
IgM
Both IgG and IgM
PRA (%) - Most Recent Class I: Enter the Class I PRA (%) value obtained from the most recent
serum. This field is required. If percentage is unavailable, select the reason from the status (ST)
drop-down list (N/A, Not Done, Missing, Unknown). (List of Status codes)
Anti-HLA Interpretation - Most Recent Class I: Select the result from the drop-down list. This field
is required. (List of Anti-HLA Interpretation codes - Class I)
Class I antibody present
No Class I antibody present
Unknown
Was serum screened for anti-HLA Class II antibody: If serum was screened for anti-HLA Class II
antibody, select Yes. If not, select No. This field is required. If Yes is selected, complete the Class II
section.
Serum Date - Most Recent Class II: Enter the date the serum was most recently collected and
tested to obtain Class II antigen results before the transplant was performed. Use the standard 8-digit
numeric format of MM/DD/YYYY. This field is required. If date is unavailable, select the reason from
the status (ST) drop-down list (N/A, Not Done, Missing, Unknown). (List of Status codes)
Target - Most Recent Class II: Select the target from the drop-down list. This field is required. (List
of PRA Target codes)

Cells
Purified HLA antigens, pooled
Purified HLA antigens from individual phenotypes
Purified single HLA antigens
Technique - Most Recent Class II: Select the technique from the drop-down list. This field is
required. If Other, specify is selected, enter the technique in the Specify field. If Other, specify is
selected, the Specify field is required. (List of PRA Technique codes)
Cytotoxity testing - extended incubation
Cytotoxity testing - wash
Cytotoxity testing - wash and extended incubation
Cytotoxity testing - AHG
Flow cytometry with cell targets
Flow cytometry with bead targets
ELISA
Other, specify
Technique Measures - Most Recent Class II: Select the technique from the drop-down list. This
field is required. (List of PRA Measure codes)
IgG
IgM
Both IgG and IgM

PRA (%) - Most Recent Class II: Enter the Class II PRA (%) value obtained from the most recent
serum. This field is required. If percentage is unavailable, select the reason from the status (ST)
drop-down list (N/A, Not Done, Missing, Unknown). (List of Status codes)
Anti-HLA Interpretation - Most Recent Serum Class II: Select the result from the drop-down list.
This field is required. (List of Anti-HLA Interpretation codes - Class II)
Class II antibody present
No Class II antibody present
Unknown
B. Peak Complete the peak anti-HLA Class I antibodies and PRA data for the Class I section. If the
serum was screened for anti-HLA Class II antibodies then complete the Class II section.
Were any sera tested pre-transplant that contain anti-HLA Class I antibody: If sera was tested
prior to the transplant that contained anti-HLA Class I antibodies, select Yes. If not, select No.
Serum Date - Peak Serum Class I antibody: If sera was tested prior to the transplant that
contained anti-HLA Class I antibodies, enter the date for the highest PRA value from all tested
sera. If date is unavailable, select the reason from the status (ST) drop-down list (N/A, Not Done,
Missing, Unknown). (List of Status codes)
Note:

Give the date this serum was collected. If two or more sera with different dates have the
same peak PRA, use the most recent date. Use the standard 8-digit numeric format of
MM/DD/YYYY. If only one PRA determination has been done, transcribe the date and all
other information from the Most Recent Serum section to the Peak Serum section.

Target - Peak Serum Class I: Select the target from the drop-down list. (List of PRA Target
codes)

Cells
Purified HLA antigens, pooled
Purified HLA antigens from individual phenotypes
Purified single HLA antigens
Technique - Peak Serum Class I: Select the technique from the drop-down list. If Other,
specify is selected, enter the technique in the Specify field. (List of PRA Technique codes)
Cytotoxity testing - extended incubation
Cytotoxity testing - wash
Cytotoxity testing - wash and extended incubation
Cytotoxity testing - AHG
Flow cytometry with cell targets
Flow cytometry with bead targets
ELISA
Micro Array
Other, specify
Measures - Peak Serum Class I: Select the technique from the drop-down list. (List of PRA
Measure codes)
IgG
IgM
Both IgG and IgM
PRA (%) - Peak Serum Class I: Enter the Peak Class I PRA (%) value obtained from the
peak serum. If percentage is unavailable, select the reason from the status (ST) drop-down
list (N/A, Not Done, Missing, Unknown).

Anti-HLA Interpretation - Peak Serum Class I: Select the result from the drop-down list.
(List of Anti-HLA Interpretation codes - Class I)
Class I antibody present
No Class I antibody present
Unknown
Were any sera tested pre-transplant that contain anti-HLA Class II antibody: If sera was tested
prior to the transplant that contained anti-HLA Class II antibodies, select Yes. If not, select No.
Serum Date - Peak Serum Class II antibody: If sera was tested prior to the transplant that
contained anti-HLA Class II antibodies, enter the date for the highest PRA value from all tested
sera. If date is unavailable, select the reason from the status (ST) drop-down list (N/A, Not Done,
Missing, Unknown). (List of Status codes)
Note:

Give the date this serum was collected. If two or more sera with different dates have the
same peak PRA, use the most recent date. Use the standard 8-digit numeric format of
MM/DD/YYYY. If only one PRA determination has been done, transcribe the date and all
other information from the Most Recent Serum section to the Peak Serum section.

Target - Peak Serum Class II: Select the target from the drop-down list. (List of PRA Target
codes)

Cells
Purified HLA antigens, pooled
Purified HLA antigens from individual phenotypes
Purified single HLA antigens
Technique - Peak Serum Class II: Select technique from the drop-down list. If Other,
specify is selected, enter the technique in the Specify field. (List of PRA Technique codes)
Cytotoxity testing - extended incubation
Cytotoxity testing - wash
Cytotoxity testing - wash and extended incubation
Cytotoxity testing - AHG
Flow cytometry with cell targets
Flow cytometry with bead targets
ELISA
Micro Array
Other, specify
Measures - Peak Serum Class II: Select the measure from the drop-down list. (List of PRA
Measure codes)
IgG
IgM
Both IgG and IgM
PRA (%) - Peak Serum Class II: Enter the Peak Class I PRA (%) value obtained from the
peak serum. If percentage is unavailable, select the reason from the status (ST) drop-down
list (N/A, Not Done, Missing, Unknown). (List of Status codes)
Anti-HLA Interpretation - Peak Serum Class II: Select the anti-HLA interpretation from the
drop-down list. (List of Anti-HLA Interpretation codes - Class II)
Class I antibody present
No Class II antibody present
Unknown

Section III - Crossmatch
Note: If Yes is selected for Crossmatch Done, the fields in Section III are required.
A. Most Recent
Date of crossmatch serum: Enter the date of the serum that was most recently collected and tested
to obtain crossmatch results before the transplant was performed. Use the standard 8-digit numeric
format of MM/DD/YYYY.
Cell Type: Select the cell type from the drop-down list. (List of Cell Type codes)
T-Cells
B-Cells
Unseparated lymphocytes
Purified Class I antigen
Purified Class II antigen
Purified Class I and II antigen
Platelets
Monocytes
Endothelial cells
Target: Select the source from the drop-down list. (List of Target Cell Source codes)
Peripheral Blood
Lymph Nodes
Spleen
Thymocytes
Cell lines/clonal cells
Solid Matrix
Note: To de-select an option in the Target field, hold down the Ctrl key on your keyboard and
click the option you want removed.
Technique: Select the technique from the drop-down list. If Other, specify is selected, enter the
technique in the Specify field. (List of Histocompatibility Technique codes)
NIH/Extended
Wash/Extended
Anti-Globulin
FLow
ELISA
Other, specify
Measures: Select the measure from the drop-down list. (List of PRA Measure codes)
IgG
IgM
Both IgG and IgM
Result: Select the crossmatch results from the drop-down list. (List of Crossmatch Results codes)
Intermediate
Negative
Positive
Weak Positive
AutoXM Result Using This Target and Technique: Select the AutoXM result from the drop-down
list. (List of Auto-Crossmatch Results codes)

Positive
Negative
Indeterminate
Not Tested
Unknown
B. Date of crossmatch serum - Least Recent (for reference purposes): Enter the date of the least
recent crossmatch serum in the space provided. Use the standard 8-digit numeric format of
MM/DD/YYYY. If only one date of crossmatch serum is available, enter the same date for Most Recent
and Least Recent.
C. Positive crossmatch with sera other than the most recent by any method: If there was a
positive crossmatch , select Yes. If not, select No. If Yes, complete the following section:
Serum Date: If there was a positive crossmatch with sera other than the most recent by any method,
enter the date of the most recent positive serum. Use the standard 8-digit numeric format of
MM/DD/YYYY.
Cell Type: Select the cell type from the drop-down list. (List of Cell Type codes)
T-Cells
B-Cells
Unseparated lymphocytes
Purified Class I antigen
Purified Class II antigen
Purified Class I and II antigen
Platelets
Monocytes
Endothelial cells
Target: Select the source from the drop-down list. (List of Target Cell Source codes)
Peripheral Blood
Lymph Nodes
Spleen
Thymocytes
Cell lines/clonal cells
Solid Matrix
Technique: Select the technique from the drop-down list. If Other, specify is selected, enter the
technique in the Specify field. (List of Histocompatibility Technique codes)
NIH/Extended
Wash/Extended
Anti-Globulin
FLow
ELISA
Other, specify
Measures: Select the measure from the drop-down list. (List of PRA Measure codes)
IgG
IgM
Both IgG and IgM
NEG XM by any other technique with this serum: If a negative crossmatch was obtained, select
Yes. If not, select No. If unknown, select UNK.
AutoXM Result Using This Target and Technique: Select the AutoXM result from the drop-down
list. (List of Auto-Crossmatch Results codes)

Positive
Negative
Indeterminate
Not Tested
Unknown
D. Autocrossmatch results:
Has autocrossmatch ever been positive: Select the answer from the drop-down list.
Yes
No
Unknown
Not Tested
If Yes is selected, enter the date the AutoXM Date using the standard 8-digit numeric format of
MM/DD/YYYY.
Section IV - Donor Retyping
Note: If Yes is selected for Donor Retyped at Your Center, the fields in Section IV are required.
Donor Retyped Class I: If the donor was retyped for anti-HLA Class I antigens, select Yes. If not,
select No. If unknown, select Unk.
If Yes is selected, Donor A, B, Bw4, Bw6, and Cw HLA values, selected in DonorNet® or on the
Donor Histocompatibility record, display.
Enter the Date Typing Completed for Class I using the standard 8-digit numeric format of
MM/DD/YYYY.
Target Cell Source Class I: Select the source from the drop-down list. (List of Target Cell Source
codes)
Peripheral Blood
Lymph Nodes
Spleen
Thymocytes
Cell lines/clonal cells
Solid Matrix
Typing Method Class I: Indicate if the typing method is Serology and/or DNA. If a typing for Class
I was done, complete the antigen section. (List of Typing Method codes)
Note: If donor HLA values were selected on the donor record in DonorNet or on the Donor
Histocompatibility record, the previously entered Typing Method Class I antigens display for
your reference.
Select the antigens from the drop-down lists. If the second antigen at a locus is blank, select No
second antigen detected. If the donor was not tested for a particular antigen, select Not tested.

Note: If the split of an antigen is known, enter the split rather than the parent.
A Locus Codes: (List of A Locus codes) 1, 2, 3, 9, 10, 11, 19, 23, 24, 25, 26, 28, 29, 30, 31,
32, 33, 34, 36, 43, 66, 68, 69, 74, 80, 203, 210, 2403, 6601, 6602, Unknown, No second
antigen detected, Not Tested
B Locus Codes: (List of B Locus codes) 5, 7, 8, 12, 13, 14, 15, 16, 17, 18, 21, 22, 27, 35, 37,
38, 39, 40, 41, 42, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63,

64, 65, 67, 70, 71, 72, 73, 75, 76, 77, 78, 81, 703, 804, 1304, 2708, 3901, 3902, 3905, 4005,
5102, 5103, 8201, Unknown, No second antigen detected, Not Tested

Bw4 HLA Codes: (List of Workgroup HLA codes) Positive, Negative, Not Tested
Bw6 HLA Codes: (List of Workgroup HLA codes) Positive, Negative, Not Tested
Cw HLA Codes: (List of Cw HLA codes) 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 13, 14, 15, 16,
17, 18, No second antigen detected, Not tested, No antigen detected
Donor Retyped Class II: If the donor was retyped for anti-HLA Class II antigens, select Yes. If not,
select No. If unknown, select Unk.
If Yes is selected, donor DR, DR51, DR52, DR53, DQ, and DPW HLA values, selected on the
donor record in DonorNet or on the Donor Histocompatibility record, display.
Enter the Date Typing Completed for Class II using the standard 8-digit numeric format of
MM/DD/YYYY.
Target Cell Source Class II: Select the source from the drop-down list. (List of Target Cell Source
codes)
Peripheral Blood
Lymph Nodes
Spleen
Thymocytes
Cell lines/clonal cells
Solid Matrix
Typing Method Class II: Indicate if the typing method is Serology and/or DNA. If a typing for
Class II was done, complete the antigen section. (List of Typing Method codes)
Note: If donor HLA values were selected on the donor record in DonorNet or on the Donor
Histocompatibility record, the previously entered Typing Method Class II antigens display for
your reference.
Select the antigens from the drop-down lists. If the second antigen at a locus is blank, select No
second antigen detected. If the donor was not tested for a particular antigen, select Not tested.

Note: If the split of an antigen is known, select the split rather than the parent.
DR Locus Codes: (List of DR Locus codes) 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14,
15, 16, 17, 18, 103, 1403, 1404, No second antigen detected, Not tested
DR51 HLA Code: (List of Workgroup HLA codes) Positive, Negative, Not Tested
DR52 HLA Code: (List of Workgroup HLA codes) Positive, Negative, Not Tested
DR53 HLA Code: (List of Workgroup HLA codes) Positive, Negative, Not Tested
DQ HLA Codes: (List of DQ HLA codes) 1, 2, 3, 4, 5, 6, 7, 8, 9, No second antigen detected,
Not tested,
DPW HLA Codes: (List of DP HLA codes) 1, 2, 3, 4, 5, 6, No second antigen detected, Not
tested
Note:

Not tested is not a valid option for the DR locus codes for kidney, pancreas or
kidney/pancreas recipients on a kidney record.