Saliva Processing Protocol

Attach 20. Saliva Processing Protocol.docx

Stress and Cortisol Measurement for the National Childrens Study (NICHD)

Saliva Processing Protocol

OMB: 0925-0671

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ATTACHMENT 20 SALIVA PROCESSING PROTOCOL



Measurement of Maternal Life Experience Study

Saliva Processing Protocol


Saliva Processing Instructions:

  • Follow Universal precautions when handling samples

  • Affix aliquot labels to 2.0ml cryovials.

  • Spin saliva tubes at 2000g (3100 rpm) for 15 minutes.

  • Remove salivette from inside tube and discard.

  • Transfer 1.5ml of saliva (or whatever amount was collected) into a 2.0ml cryovial, DO NOT OVER FILL, expansion room is needed when freezing sample. Cap vial with clear cryovial cap.

  • Log all collected samples onto the MOM-le Study Saliva Specimen Log.

  • Specimen volumes less than 500ul must be documented on the Saliva Specimen Log.



Saliva Tubes Storage Instructions:

  • Label the cardboard box on the OUTSIDE with the study code and box #.

  • Store aliquots at -80ᵒC or -70ᵒC and place in cardboard storage box.



Saliva Tubes Shipping Instructions:

At the end of the study, send full saliva sample boxes on dry ice with copies of Saliva Specimen Log to:

Dr. Frank Zaldivar

101 The City Drive South, BLDG 55-334

Orange, CA 92868

p: 714.456.8248

f: 714.456.3417

e: [email protected]



***Please contact Dr. Zaldivar in advance to arrange shipping

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File Typeapplication/vnd.openxmlformats-officedocument.wordprocessingml.document
AuthorKristina Nelson
File Modified0000-00-00
File Created2021-01-30

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