4. PATH analyte by specimen and biomarker

Population Assessment of Tobacco and Health (PATH) Study (NIDA)

Attachment 4
Additional Information on Biospecimens
November 16, 2012

Population Assessment of Tobacco and Health (PATH) Study (NIDA)

PATH Biospecimen Analytes by Specimen and Biomarker
Analyte

Preferred Matrix
Tobacco Biomarkers

Biomarker

Nicotine and nicotine metabolites
Tobacco user: cotinine and trans-3'hydroxycotinine
Non-tobacco user: cotinine

Serum

Exposure

Nicotine and nicotine metabolites
Tobacco user: cotinine, trans-3'-hydroxycotinine,
cotinine N-oxide, nicotine N-oxide, nornicotine,
norcotinine; analogues: anabasine, anatabine
Non-tobacco user: cotinine and trans-3'hydroxycotinine

Urine

Exposure

Tobacco specific nitrosamines (TSNAs): NNAL,
NNN, NNA, NAT, NAB, NNK

Urine

Exposure

Polycyclic aromatic hydrocarbons (pyrene,
naphthalene, phenanthrene, fluorene)

Urine

Combustion

Cadmium, cobalt, uranium, lead, strontium,
beryllium, manganese, and thallium

Urine, prescreened
container

Toxicity

Speciated arsenic (As III, As V, dimethylarsinic acid
(DMA), and monomethylarsonic acid (MMA))

Urine, prescreened
container

Toxicity

Creatinine

Urine
Red blood cells,
EDTA

4-ABP hemoglobin

For correction
Toxicity

VOCs metabolites

Urine

Combustion

Volatile nitrosamines

Urine

Combustion

Aromatic amines

Urine

Combustion

Cyanide

Urine

Combustion

C-reactive protein

Other Biomarkers
Serum, red top or
SST

Inflammation;
Cardiovascular risk

Fibrinogen

Plasma, citrate

Cardiovascular risk

Interleukin 6

Plasma

Inflammation

sICAM (soluble intercellular adhesion molecule)

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Plasma, EDTA or
Serum, SST

Cardiovascular risk

Population Assessment of Tobacco and Health (PATH) Study (NIDA)

F2-isoprostane / 8-epi-prostaglandin F2a

Urine

Oxidative stress

MicroRNA profile

Plasma

Epigenetic effects

Metabolomic profile

Plasma, EDTA

Metabolic effects

Proteomic profile

Plasma

Toxicity, risk, stress

DNA (genotyping, sequencing)

Buffy coat

Role of genetics

RNA (gene expression)

PAXgene

Epigenetic effects

Metabolomic profile

Urine

Metabolic effects

microRNA, total RNA

Buccal cells

Epigenetic effects

Epigenetic marks

Buccal cells

Epigenetic effects

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