6 Additional Information on biospecimens 6-7-13

Population Assessment of Tobacco and Health (PATH) Study (NIDA)

Attachment 6
Additional Information on Biospecimens
June 18, 2013

Population Assessment of Tobacco and Health (PATH) Study (NIDA)

PATH Biospecimen Analytes by Specimen and Biomarker
Analyte
Preferred Matrix
Tobacco Biomarkers In Tobacco Users and Non-tobacco Users

Biomarker

Nicotine and nicotine metabolites
Tobacco user: cotinine and trans-3'hydroxycotinine
Non-tobacco user: cotinine

Serum

Exposure

Nicotine and nicotine metabolites
Tobacco user: cotinine, trans-3'-hydroxycotinine,
cotinine N-oxide, nicotine N-oxide, nornicotine,
norcotinine; analogues: anabasine, anatabine
Non-tobacco user: cotinine and trans-3'hydroxycotinine

Urine

Exposure

Tobacco specific nitrosamines (TSNAs): NNAL,
NNN, NNA, NAT, NAB, NNK

Urine

Exposure

Polycyclic aromatic hydrocarbons (pyrene,
naphthalene, phenanthrene, fluorene)

Urine

Combustion

Cadmium, cobalt, uranium, lead, strontium,
beryllium, manganese, and thallium

Urine, prescreened
container

Speciated arsenic (As III, As V, dimethylarsinic
acid (DMA), and monomethylarsonic acid (MMA))
Creatinine
4-ABP hemoglobin

Toxicity

Urine, prescreened
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Toxicity

Urine

For correction

Red blood cells, EDTA

Toxicity

VOCs metabolites

Urine

Combustion

Volatile nitrosamines

Urine

Combustion

Aromatic amines

Urine

Combustion

Cyanide

Urine

Combustion

Other Biomarkers in Tobacco Users and Non-tobacco Users
C-reactive protein

Serum, red top or SST

Fibrinogen
Interleukin 6
sICAM (soluble intercellular adhesion
molecule)
F2-isoprostane / 8-epi-prostaglandin F2a
MicroRNA profile

1

Inflammation;
Cardiovascular risk

Plasma, citrate

Cardiovascular risk

Plasma

Inflammation

Plasma, EDTA or
Serum, SST

Cardiovascular risk

Urine

Oxidative stress

Plasma

Epigenetic effects

Population Assessment of Tobacco and Health (PATH) Study (NIDA)

Metabolomic profile

Plasma, EDTA

Proteomic profile

Plasma

Metabolic effects
Toxicity, risk, stress

DNA (genotyping, sequencing)

Buffy coat

Role of genetics

RNA (gene expression)

PAXgene

Epigenetic effects

Metabolomic profile

Urine

Metabolic effects

microRNA, total RNA

Buccal cells

Epigenetic effects

Epigenetic marks

Buccal cells

Epigenetic effects

2