EPA 6400-01 (OMB 2 NPDES Permittee Data Report Form

PRE-SORTED
FIRST-CLASS MAIL
POSTAGE & FEES PAID
EPA
PERMIT NO. G-35

OECA MC 2227A
WASHINGTON DC 20460
Official Business
Penalty for Private Use $300

TIME SENSITIVE - RESPONSE NEEDED
IMPORTANT DATES TO REMEMBER
March 21, 2014:
July 11, 2014:
August 29, 2014:
October 10, 2014:

Submit address verification and order PT Samples
DMR-QA Study 34 ends
Submit DMR-QA 34 Results to DMR-QA Coordinator
Address non acceptable results and submit retest
samples to DMR-QA Coordinator

IMPORTANT NOTICE TO NPDES PERMITTEES
DMR-QA Study 34 Announcement

DMR-QA STUDY 34
Immediately verify receipt of DMR-QA Study 34 by either filling out the form below and mailing this page to your state
coordinator (listed on pages 6-8) or follow the e-mail instructions at the bottom of this page.
The mailed form must be postmarked on or before March 21, 2014.
NPDES PERMITTEE ADDRESS VERIFICATION FORM
Discharge Monitoring Report - Quality Assurance (DMR-QA) Study 34
Please provide corrections to the mailing address where all DMR-QA paperwork should be sent.

State

NPDES Permit Number

(2-character State Code + 7 digit Permit Code as shown on the mailing label, for
example CA1234567)

If Address is correct, you only need to check this box

Facility Name
Contact Name

Title

Mailing Address
City

State

Phone Number

Fax Number

Zip Code

E-mail Address

ELECTRONIC NOTIFICATION PROCEDURE
You may verify receipt electronically by sending an e-mail on or before March 21, 2014 to your state DMR-QA coordinator
(listed on pages 6-8 of the enclosed instructions). The e-mail should be composed in the following manner:
1.
2.

Subject line should contain ONLY the NPDES Permit number (2-character State Code + 7-digit Permit Code as shown on the mailing
label, for example CA1234567). Other information is not needed on the subject line.
If you require any changes to the mailing label on this announcement, the body of the e-mail should contain a list including:
Company name, Contact Name/Title, Mailing Address, City, State, Zip Code, Facility Type (select one: federal, state, local or
commercial/private). Otherwise, you may simply write “No changes to address” in the body of the e-mail.

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY
WASHINGTON, DC 20460
OFFICE OF
ENFORCEMENT AND
COMPLIANCE ASSURANCE

Dear NPDES Permit Holder:
This letter initiates the 2014 Discharge Monitoring Report - Quality Assurance (DMR-QA) Study 34. By receipt of this
letter, you are required, under Clean Water Act (CWA) Section 308, to participate in DMR-QA Study 34 unless your
facility is exempted by an EPA waiver.1 Your participation plays a key role in monitoring the quality of data used to
assure the integrity of the CWA's National Pollutant Discharge Elimination System (NPDES) program.
DMR-QA Study 34 covers major and select minor NPDES permit holders. You (the permittee) are responsible for
ensuring results of DMR-QA Study tests, performed by your in-house and/or contract laboratories, are graded by an
accredited Proficiency Testing (PT) Provider. If any graded results are "Not Acceptable," you must follow up with the
laboratory to determine the cause of the deficiency and ensure corrective action is taken to prevent future
occurrences. To satisfy DMR-QA Study requirements, your laboratory may use reporting data from a Water
Pollution (WP) study administered by an accredited PT Provider and performed between January 1, 2014 and
July 11, 2014. This provides flexibility to you and your laboratories performing DMR-QA Study tests. While
performing tests and analyses, please ensure that your test methods/procedures follow 40 CFR 136 guidelines. Use
the same personnel, equipment, test methods and procedures as you would for routine DMR reporting.
What changes were made to DMR-QA Study 34?
The Whole Effluent Toxicity (WET) checklist was updated to reflect recent method updates. Labs should no longer use
a No Observable Effect Concentration (NOEC) Survival endpoint for short-term chronic tests. The Chemistry/
Microbiology analyte checklist has a column for writing in the test method number used. Also new for this year, there
is a space in the NPDES Permittee Data Report form to enter WP Study information used in lieu of DMR-QA. Supplying
information in these columns is optional but encouraged. Permittees with valid e-mail addresses will receive the
DMR-QA Study by e-mail, but may request a hard copy.
Further information
Questions on the national program should be addressed to Brian Krausz ([email protected], 202-564-3069), EPA's
National DMR-QA Coordinator. State and EPA regional DMR-QA contact information is provided on pages 6-8. Please
reference your NPDES permit number on all correspondence.
Thank you for your attention to this Clean Water Act Section 308 requirement.
Sincerely,

Enclosures

Edward J. Messina, Director
Monitoring, Assistance, and Media Programs Division
Office of Compliance

1

EPA is authorized to collect this information under Section 308 of the Clean Water Act, 33 U.S.C. § 1318. This information request is
enforceable under 33 U.S.C. § 1319. EPA may grant a waiver from participating in DMR-QA to states with laboratory quality assurance
programs approved by EPA as a substitute for the DMR-QA Study. Refer to the footnote on page 2 to determine if you are covered by an EPA
waiver, or contact your state coordinator.
Internet Address (URL) · http://www.epa.gov

DMR-QA Study 34
Table of Contents and Schedule
DMR-QA Study 34 Frequently Asked Questions
NPDES Permittee Instructions
Chemistry and Microbiology Laboratory Instructions
WET Testing Laboratory Instructions
Accredited Proficiency Testing Providers
EPA and State Regional DMR-QA Coordinators
NPDES Permittee Data Report Form
Chemistry/Microbiology Analyte Checklist
WET Organisms/Test Conditions/End Points Checklist

2
3
4
5
6
6
9
12
13

Checklist and Schedule for DMR-QA Study 34
Deadline*
March 21, 2014

Permittee
Study 34 begins
Send Address Verification Form (inside cover
of this package) to the state coordinator by email or postal mail to confirm receipt of Study
34 Announcement

In-House and Contract Laboratories

PT Providers

Study 34 begins
Order test samples from PT
Provider

Notify all laboratories of DMRQA Study 34
July 11, 2014

Study 34 ends

Study 34 ends
Send ungraded Data Report to PT
Providers (include a list of all NPDES
permits using your laboratory data)
Send PT Provider-graded test results, for
each permit (listed by NPDES permit
numbers), to:
- Laboratory
- State DMR-QA coordinators

August 1, 2014

Forward PT Provider-graded test results to
the Permittee

August 15, 2014

August 29, 2014

October 10, 2014

Forward one copy of the NPDES Permittee
Data Report Form, and a copy of the
Chemistry/Microbiology and WET
Checklists for each laboratory used to the
state DMR-QA coordinator

Order testing samples from PT Provider for
all "Not Acceptable" analyte test results
Complete and send required corrective
action letter as soon as possible to your
Permittee

Address any "Not Acceptable" data by
submitting a completed and signed
corrective action report to your state
DMR-QA coordinator

* All materials must be sent on or before the date provided.
Page 1

DMR-QA STUDY 34 - Frequently Asked Questions
Am I required to participate in this DMR-QA Study?
NPDES Major and select Minor permit holders are required to participate in DMR-QA studies. Be sure to verify the permit number on
the front of this package. If you believe you received this study in error, contact your state DMR-QA coordinator (pages 6-8). Some
permit holders are not required to perform the DMR-QA study because they are located in states that have laboratory quality
assurance programs approved by EPA as a substitute for DMR-QA.2
What is the purpose of this DMR-QA Study?
The purpose of the Discharge Monitoring Report - Quality Assurance (DMR-QA) Study 34 is to ensure the integrity of data submitted
by the permittee for DMR reporting requirements and evaluate the performance of the laboratories to analyze wastewater samples.
What laboratory tests are required under DMR-QA?
Permittees are responsible for having their laboratory(ies) test wastewater analytes that are both in their NPDES permit and included
in Study 34. For required Whole Effluent Toxicity (WET) tests, permittees must participate even if the test conditions (e.g.,
temperature, time of acute test, synthetic seawater matrix, etc.) in the permit do not exactly match those in Study 34. Refer to "WET
Testing Laboratory Instructions" (page 5) for more information.
How should laboratory personnel perform DMR-QA tests?
For all pollutant parameters, especially field test parameters (e.g., pH, residual chlorine), use the same personnel, equipment, and
procedures as required for NPDES compliance monitoring analyses. Ensure that your test methods and procedures follow 40 CFR 136
guidelines.
Where can laboratories get test samples?
Laboratories (in-house and contract) may order the required DMR-QA Study 34 samples from a PT Provider accredited by the
American Association for Laboratory Accreditation (A2LA), or ACLASS, a brand of the ANSI-ASQ National Accreditation Board. A list of
chemistry, microbiology and WET accredited PT Providers is on page 6.
What should permittees and laboratories do first?
Permittees must first confirm receipt of this package by March 21, 2014 to their state coordinator (pages 6-8) via postal mail or e-mail.
Permittees must also send a copy of the enclosed instructions/checklists to all in-house and contract laboratories. The permittee
specifies on the checklist which analyses the laboratory will perform. See Permittee Instructions (page 3) for more information. Upon
receipt of instructions, laboratories must order samples from an accredited PT Provider (page 6). This should be done early to allow
sufficient time to perform the required analyses and send ungraded results to the PT Provider prior to the close of DMR-QA Study 34
(July 11, 2014). See Laboratory Instructions (pages 4-5) for more information.
Can I use a Water Pollution (WP) Study to satisfy DMR-QA requirements?
Yes, permittees may use a WP study to satisfy some or all of the DMR-QA requirements. However, only results from WP studies
closing between January 1 and July 11, 2014 will be accepted. See Permittee Instructions for more information (Step 5 on page 3).
What steps do I take after the proficiency testing?
After laboratories report their data to the Proficiency Test (PT) Providers, the PT Provider issues a report to the laboratory and the
state DMR-QA coordinator, indicating the results were "Acceptable" or "Not Acceptable". The laboratory must then forward a copy of
the graded report to the permittee. The permittee subsequently completes the checklists on pages 12-13, indicating the laboratory's
grade for each required analyte. One set of checklists must be used for each laboratory. The permittee must also fill out the NPDES
Permittee Data Report Form (EPA Form 6400-01, pages 9-11) and submit a signed copy with the completed checklists and copies of
the laboratory's graded reports to the state coordinator by August 29, 2014.
What do I do if a laboratory receives a "Not Acceptable" result?
If any graded results are "Not Acceptable," the permittee must follow up with the laboratory to determine the cause of the deficiency
and ensure corrective action is taken to prevent future occurrences. The laboratory must order retesting samples, reanalyze them,
and write a corrective action response for the permittee. The permittee is required to submit a completed and signed corrective
action report to the state DMR-QA coordinator by October 10, 2014.
Where do I go for more information?
DMR-QA resources including fill-and-print forms are available at www.epa.gov/compliance/monitoring/programs/cwa/dmr/.
2 As of January 1, 2014, permittees in California, Kansas, New Jersey, Nevada, North Carolina, Pennsylvania, South Carolina, Virginia, West Virginia

and Wisconsin have laboratory quality assurance programs approved by EPA as a substitute for DMR-QA. Louisiana has an approved laboratory
quality assurance programs for commercial laboratories only. NPDES permittees in Arkansas and Oklahoma that currently participate in their State
laboratory accreditation program are also exempted from DMR-QA Study 34. Laboratories in Oklahoma and Arkansas not certified by the State must
perform DMR-QA studies. Check with your state coordinator if you have questions about your state's exemption status.

Page 2

NPDES Permittee Instructions
1.

Verify your participation in DMR-QA Study 34 by confirming the NPDES permit number on the front of this package. If you believe
you received this study in error or have questions about your exemption status, contact your state DMR-QA coordinator (pages 6-8).
Please refer to Footnote 2 of the Frequently Asked Questions page (page 2) for a list of exempted states.

2. Follow the instructions on the DMR-QA Study 34 Important Notice (inside front cover of study package) and immediately confirm
receipt of this package to your state coordinator via postal mail or e-mail. You must submit your response no later than March 21,
2014. If your permit is inactive, please contact your state DMR-QA coordinator immediately.
3.

Send copies of these instructions to each contract and in-house laboratory, if applicable. Ensure DMR-QA samples are analyzed by the
same laboratories that routinely perform analyses for your Discharge Monitoring Report (DMR) requirements. Indicate which tests the
laboratory will perform by checking the appropriate boxes in the "Test Required" column in the enclosed tables (pages 12-13).

4.

Ensure that each laboratory uses their U.S. EPA-assigned Laboratory Code on all reported results. Make certain your laboratories
understand and complete all requirements. Laboratories needing a new U.S. EPA Lab Code or wanting to confirm their existing U.S.
EPA Lab Code, should contact:
Charles Feldmann, US EPA
26 W. Martin Luther King Drive, M/S 140
Cincinnati, OH 45268
Tel: (513) 569-7671 Fax: (513) 569-7191 E-mail: [email protected]
If you send an e-mail, include your phone number in the message. Submit all requests for lab codes at least one week before the PT
Providers' WP study due date or one week prior to the DMR-QA Study 34 end date (July 11, 2014) to allow time for response.

5.

Instruct your laboratory to order samples for analytes that are both in your permit and included in DMR-QA Study 34. Your in-house
and contract laboratories must order PT samples from an accredited PT Provider (page 6). Ensure your laboratory orders the samples
early enough to allow time to perform the required analyses and send results to the PT Provider prior to the close of DMR-QA Study
34 (July 11, 2014). Your laboratory should register your permit number with the PT Provider prior to July 11, 2014. Note: If your inhouse or contract laboratory chooses to use a WP study to satisfy DMR-QA requirements, please inform the laboratory that all data
must be reported to the PT Provider by the published WP study close date, even if it is prior to the DMR-QA deadline of July 11, 2014.
WP studies are only valid for DMR-QA Study 34 if the WP study meets the following requirements:
a) Samples are offered by an accredited PT Provider (page 6).
b) The WP study does not close before January 1, 2014, or after July 11, 2014.
c) The PT Provider shows the WP results from each of the permittee's regulated analytes on the DMR-QA reporting form.

6.

Permittees are responsible for ensuring that laboratories submit data on permittee's behalf to the PT Provider by the end of DMR-QA
Study 34 (July 11, 2014). Laboratories must send ungraded data to the same PT Provider they received samples from. By August 1,
2014, PT Provider-graded test results will be sent back to the laboratories. Permittees will not receive graded reports directly from PT
Providers unless they are an in-house laboratory. Note: Permittees are not required to report ungraded data to the PT Provider on
their laboratories' behalf nor should permittees send ungraded data to their state DMR-QA coordinator.

7.

Permittees must require laboratories to forward to them, the graded results from the PT Provider by August 15, 2014. Using these
graded results, permittees must fill out the Chemistry/Microbiology Analyte and WET Organisms/Test Conditions/End Points
checklists (pages 12-13) for each laboratory, indicating the analyte tests performed by the laboratory and whether the result was
Acceptable or Not Acceptable. Make sure the appropriate NPDES permit number and U.S. EPA Lab Code are on each checklist. If you
use more than one laboratory, you must use a separate checklist for each laboratory. If a laboratory reports more than one method to
you for any single analyte, you must use a separate checklist for each method reported.

8.

Follow the directions on the "NPDES Permittee Data Report Form" (pages 9-11) and complete the information. You may use a "fill and
print" form available at: www.epa.gov/compliance/monitoring/programs/cwa/dmr/. By August 29, 2014, you must send a copy
of the signed NPDES Permittee Data Report Form, graded results, and completed checklists from step 7 for each of your permits to
your state DMR-QA coordinator. However, if the PT Provider already submits graded results sorted by permit number to the state
DMR-QA coordinator, the permittee is only required to send copies of the checklist and EPA Form 6400-01. In this situation, the
laboratory should provide a list of its permittees' permit numbers associated with each required analyte to the PT Provider prior to the
close of DMR-QA Study 34 or the WP study. Check with your laboratory to determine whether the PT Provider is sending the graded
data directly to the state DMR-QA coordinator. Permittees must maintain a copy of the completed NPDES Permittee Data Report
Form, checklists and graded laboratory results as a record for at least three (3) years.

9. After receiving laboratory results, permittees must consult with the laboratory and investigate any discrepancies or "Not Acceptable"
evaluations reported by the PT Provider. Permittees must identify and report to the state DMR-QA coordinator causes and system
changes to correct the discrepancies, and avoid their reoccurrence. Laboratories must order retesting samples by August 29, 2014
for any "Not Acceptable" results and perform the verification analysis as soon as possible to include the results in the Corrective
Action report to be sent to the state DMR-QA coordinator before October 10, 2014. The report must include the results of any
verification analysis performed.
Page 3

Chemistry and Microbiology Laboratory Instructions
Your laboratory is designated to participate in DMR-QA Study 34 by a National Pollutant Discharge Elimination System (NPDES)
permittee, because the permittee uses or plans to use your laboratory to perform chemistry/microbiology analyses to satisfy their
NPDES permit requirements during 2014. For measurements of all pollutant parameters, especially field test parameters (e.g., pH,
residual chlorine), use the same personnel, equipment, and procedures as required for NPDES compliance monitoring analyses.
Please ensure that your test methods/procedures follow 40 CFR 136 guidelines. Please note for low level mercury, the concentration
range is 20 - 100 ng/L (20 - 100 parts per trillion) and for low level total residual chlorine, the concentration range is 75 - 250 μg/L (75 250 parts per billion). If you have questions about whether you should perform the test using the normal or the low level mercury
and/or low level total residual chlorine concentration, contact your state DMR-QA coordinator.
1. Each permittee for whom you analyze or provide data in 2014 will determine which analyses you must perform by checking the
appropriate boxes on the enclosed "Chemistry/Microbiology Analyte Checklist" (page 12).
2. Order DMR-QA Study 34 chemistry and microbiology samples from one of the Proficiency Test (PT) Providers (page 6). Be sure to
allow yourself enough time to perform the analyses before the closing date of DMR-QA Study 34 (July 11, 2014). Maintain a copy
of all completed order forms for your records.
Helpful Hint: You may be able to utilize the results from a Water Pollution (WP) study to meet the requirements of DMR-QA, if all of
the following conditions are met:
a) Samples are offered by an accredited PT Provider (page 8).
b) The WP study does not close before January 1, 2014, or after July 11, 2014.
c) The PT Provider shows the WP study results of each permittee's regulated analytes on the DMR-QA reporting form.

3.

Record your ungraded analytical data and your EPA Lab Code on the Data Reporting Forms received with your samples. Be sure
to follow the PT Provider's instructions and deadlines received with these samples.

4. Use of the EPA-assigned Lab Code on all reported results is required. If you need a new EPA Lab Code or need to verify your
existing EPA Lab Code, contact Charles Feldmann (see contact information on page 3, step #4).
5. Send the data requested by each of your permittees to the PT Provider for grading. You must send data to the same PT Provider
that you received samples from. Make sure you provide the DMR-QA Study 34 or WP study results to the PT Provider by July 11,
2014. If you choose to use a WP study, you must report all data to the PT Provider by their published WP study close date, even if
it is prior to the DMR-QA deadline of July 11, 2014. (Notify the PT Provider that a WP study is being used for DMR-QA and send
copies of the analyte checklist(s) if you are reporting via hardcopy).
6. The PT Provider will grade your analyses and send the graded results to you by August 1, 2014. If the PT Provider is submitting
graded results sorted by permit number to the state DMR-QA coordinator, then you should register your permittees' permit
numbers associated with each required analyte with the PT Provider prior to the close of DMR-QA Study 34 or the WP study.
Forward the graded results of the analytes to the permittee by August 15, 2014, so the permittee can fill out the analyte
checklist on page 12.
7.

If corrective action is required, notify the permittee. Laboratories must order retesting samples for "Not Acceptable" analyte test
results by August 29, 2014. You must also identify and report causes and system changes to correct the discrepancies and avoid
a reoccurrence. The corrective action letter and graded retest report need to be forwarded to the permittee as soon as possible.

Page 4

WET Testing Laboratory Instructions
Your laboratory is designated to participate in DMR-QA Study 34 by a National Pollutant Discharge Elimination System (NPDES)
permittee, because the permittee uses or plans to use this laboratory to perform Whole Effluent Toxicity (WET) analyses during 2014.
For all analytes, use the same personnel, equipment, and procedures as required for NPDES compliance monitoring analyses. Ensure
that your test methods/procedures follow 40 CFR 136 guidelines and the manuals referenced below.
1.

The permittee(s) determine which analyses you will perform by checking the appropriate boxes on the enclosed table "WET
Organisms/Test Conditions/End Points" (page 13). Ensure that the permittee has selected the test organism(s) and testing
conditions that most closely resemble those required by the permit for which you are supplying test results. Use the guidelines
immediately below and the table on page 13 to select the proper WET tests.
Guidelines for Choosing the Correct WET Test Organisms/Conditions/End Points
• If the permit requires WET testing with Fathead minnows, Ceriodaphnia dubia, Daphnia magna, Daphnia pulex, Mysidopsis bahia, Inland
silverside (Menidia beryllina) or Sheepshead minnow (Cyprinodon variegates), test those organisms listed in each permit using the test
conditions, including temperature, defined in the Test Codes.
• If the permit's WET testing conditions for Ceriodaphnia dubia specify 48-h acute, non-renewal testing, conduct this test using the static, renewal
acute conditions defined by Test Codes 19 and 20. The testing conditions defined for these Test Codes have been proven to provide an
appropriate measure of your ability to perform WET testing with Ceriodaphnia dubia.
• If the permit's WET testing conditions for Daphnia magna and Daphnia pulex specify 48-h acute renewal testing, you must conduct this test
using the non-renewal conditions specified in Test Codes 32 and 38.
• If the permit's WET testing conditions require 24, 48, or 96-h acute testing using any of the organisms included in Study 34, use the 48-h acute
test conditions specified in the Test Codes.
• If the permit requires WET testing with Mysidopsis bahia, Inland silverside (Menidia beryllina) or Sheepshead minnow (Cyprinodon variegates)
and your laboratory uses an alternate synthetic seawater (e.g. Hawaiian Brands, GP2) other than the 40 Fathoms specified in the Test Codes,
you must still perform testing.
• If the permit requires 20°C acute testing for any organisms included in Study 34, use 25°C acute test conditions specified in the Test Codes.
• For laboratory performance quality assurance (QA) purposes, the point estimation techniques that produce end points such as the Inhibiting
Concentration 25% (IC25) are the preferred statistical methods in calculating end points for effluent chronic toxicity tests. However,
laboratories should choose the statistical methods that allow calculation of the end point(s) required by the NPDES permit and used for routine
permit compliance tests. For example, if a permit specifies a No Observable Effect Concentration (NOEC) end point for Growth, and it is
reported regularly on DMR's, this end point should be reported for DMR-QA.

2.

Order DMR-QA toxicity samples from an accredited PT Provider (page 6). Allow yourself enough time to perform the analyses
before the closing date of DMR-QA Study 34 (July 11, 2014). Maintain a copy of all completed order forms for your records.

3.

Record your ungraded analytical data and your EPA Lab Code number on the Data Report Form received with your samples. Be
sure to follow the PT Provider's instructions and deadlines received with the samples. Use of the EPA-assigned Lab Code on all
reported results is required. If you need a new EPA Lab Code or need to verify your current code, contact Charles Feldmann (see
contact information on page 3, step #4).

4.

Send the ungraded data requested by each of your permittees to the PT Provider for grading. You must send it to the same PT
Provider that you received samples from. Make sure you provide the DMR-QA Study 34 study results by July 11, 2014.

5.

The PT Provider will grade your results and send them to you by August 1, 2014. If the PT Provider is submitting graded results
sorted by permit number to the state DMR-QA coordinator, then you should register your permittees' permit numbers associated
with each required analyte with the PT Provider prior to the close of DMR-QA Study 34 or the WP study. Forward the graded
results of the analytes to the permittee by August 15, 2014 so the permittee can fill out the analyte checklist on page 13.

6.

If corrective action is required, notify the permittee. Laboratories must order retesting samples by August 29, 2014. You must
identify and report the causes and all system changes to correct the discrepancies and avoid a reoccurrence. This corrective
action letter and graded retest report must be forwarded to the permittee as soon as possible.

Reference Manuals: (see http://www.epa.gov/scitech/methods/cwa/wet/index.cfm)
1. Methods for Measuring the Acute Toxicity of Effluents to Freshwater and Marine Organisms, Fifth Edition, October 2002. U.S. Environmental
Protection Agency, Office of Water, Washington, DC, EPA 821-R-02-012.
2. Short-term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, Fourth Edition, October 2002. U.S.
Environmental Protection Agency, Office of Water, Washington, DC, EPA 821-R-02-013.
3. Short-term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Marine and Estuarine Organisms, Third Edition, October
2002. U.S. Environmental Protection Agency, Office of Water, Washington, DC, EPA 821-R-02-014.
4. Method Guidance and Recommendations for Whole Effluent Toxicity (WET) Testing (40 CFR Part 136). July, 2000. U.S. Environmental Protection
Agency, Office of Water, Washington, DC, EPA-821-B -00-004.
5. Short-term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to West Coast Marine and Estuarine Organisms, 1st Ed.,
1995, U.S. Environmental Protection Agency, Office of Research and Development, Cincinnati, OH, EPA/600/R-95/136. http://www.epa.gov/
eerd/methods/full_doc.pdf

Page 5

Accredited Proficiency Testing Providers
The following Proficiency Test (PT) Providers are accredited by A2LA or ACLASS. A2LA and ACLASS have each been designated a
Proficiency Testing Oversight Body (PTOB)/Proficiency Testing Provider Accreditor (PTPA) by The NELAC Institute (TNI). Find the following
PT Providers on the internet at: http://www.nelac-institute.org/ptproviders.php.

NELAC-Accredited Provider
Absolute Standards, Inc., Hamden, CT
Mr. Stephen Arpie (203) 281-2917 or (800) 368-1131,
[email protected]
Advanced Analytical Solutions, LLC, Parkersburg, WV
Fred Anderson (304) 485-6325
[email protected]

Chem

Micro

www.absolutestandards.com

X

X

www.advancedqc.com

X

X

www.sigma-aldrich.com

X

X

X

www.eraqc.com

X

X

X

www.phenova.com

X

X

X

www.nsilabsolutions.com

X

X

[email protected]

X

X

Sigma Aldrich RTC, Laramie, WY
Mrs. Jennifer Duhon (307) 742-5452 Fax: (855) 831-9211,
[email protected]
Environmental Resource Associates (ERA), Golden, CO
Rebecca Roemer (303) 463-3531 [email protected]
Audrey Cornell (303) 463-3530 [email protected]
Phenova Inc., Golden, CO
(866) 942-2978
[email protected]
nsi lab solutions, Raleigh, NC
Mr. Mark Hammersla (919) 789-3000

[email protected]
NYS DOH Environmental Laboratory Approval Program, Albany, NY
Dr. Kenneth Aldous (518) 474-7161,

WET

EPA Regional DMR-QA Coordinators
EPA Region 1
(CT, MA, ME, NH, RI, VT)
Please contact your State Coordinator.
EPA Region 2
(NJ, NY, PR, VI)
Towana Joseph
US EPA Region II, DESA/MAB
2890 Woodbridge Avenue (MS 220)
Edison, NJ 08837
(732) 321-6607
[email protected]
EPA Region 3 (DC, DE, MD, PA, VA, WV)
Peter Gold
U.S. EPA Region III (3WP42)
Water Protection Division
1650 Arch Street
Philadelphia, PA 19103
(215) 814-5236
[email protected]

EPA Region 6 (AR, LA, NM, OK, TX)
Charles Wren, Jr.
U.S. EPA Region VI
Water Enforcement Branch
1445 Ross Avenue
Suite 1200 (6EN-WC)
Dallas, TX 75202
(214) 665-6640
EPA Region 4 (AL, FL, GA, KY, MS, NC, SC, [email protected]
TN)
Ray Terhune
EPA Region 7 (IA, KS, MO, NE)
Robert Bryant
US EPA Region IV
SESD/MTSB/QAS
U.S. EPA Region VII - Water Enforcement
980 College Station Road
11201 Renner Blvd
Athens, GA 30605-2720
Lenexa, KS 66219
(706) 355-8557
(913) 551-7354
[email protected]
[email protected]
Charles Jones, Jr.
U.S. EPA Region III - Environmental
Assessment and Innovation Division
1650 Arch Street (3EA00)
Philadelphia, PA 19103
(215) 814-2734
[email protected]

EPA Region 5 (IL, IN, MI, MN, OH, WI)
Kenneth Gunter
U.S. EPA Region V
Water Division WECAB
77 W. Jackson Boulevard (WC15J)
Chicago, IL 60604
(312) 353-9076
[email protected]

EPA Region 8 (CO, MT, ND, SD, UT, WY)
Monia Ben-Khaled
U.S. EPA Region VIII
NPDES Enforcement Unit
Water Technical Enforcement Program
1595 Wynkoop St. (8ENF-W-NP)
Denver, CO 80202
(303) 312-6209
[email protected]

Page 6

EPA Region 9
(AS, AZ, CA, GU, HI, MP, NN, NV)
Liliana Christophe
U.S. EPA Region IX
75 Hawthorne Street - Mail Code WTR 7
San Francisco, CA 94105
(415) 972-3502
[email protected]
EPA Region 10 (AK, ID, OR, WA)
Robert Grandinetti
U.S. EPA Region X
NPDES Compliance Unit
309 Bradley Boulevard, Suite 115
Richland, WA 99352
(509) 376-3748
[email protected]

State DMR-QA Coordinators
Alabama
Sharon Moses
Alabama DEM
1350 Coliseum Boulevard
Montgomery, AL 36110
(334) 394-4355
[email protected]
Alaska
Sharon Morgan
Alaska Department of
Environmental Conservation
410 Willoughby Avenue, Suite 303
Juneau, AK 99801-1800
(907) 465-5530
[email protected]
America Samoa
Liliana Christophe
U.S. EPA Region IX
75 Hawthorne Street Mail Code WTR 7
San Francisco, CA 94105
(415) 972-3502
[email protected]
Arizona
Marnie Greenbie
Arizona Dept of Environmental
Quality Permits Unit, Surface
Water Section
1110 W. Washington St.
Phoenix, AZ 85007
(602) 771-4689
[email protected]
Kathryn Wangsness
Arizona DHS
Environmental Lab Licensure
250 North 17th Avenue
Phoenix, AZ 85007
(602) 364-0724
[email protected]
Arkansas*
Jane Hurley
Arkansas Department of
Environmental Quality
5301 Northshore Drive
North Little Rock, AR 72118
(501) 682-0938
[email protected]
California*
Renee Spears
Office of Information Mgmt & Analysis
State Water Resources Control Board
1001 I Street, 16-39DSacramento, CA 95814
P.O. Box 100- Sacramento, CA 95812
(916) 341-5583
[email protected]

Connecticut
Donald Gonyea
Connecticut DEP
Bureau of Materials Management and
Compliance Assurance
79 Elm Street
Hartford, CT 06106
(860) 424-3827
[email protected]
Delaware
Glenn Davis
Delaware DNREC - Water Resources Branch
89 Kings Hwy R and R Building
Dover, DE 19901
(302) 739-9946
[email protected]
District of Columbia
Adion Chinkuyu
District of Columbia Department of
Environment - Water Quality Division
1200 First Street NE, 6th Floor
Washington, DC 20002
(202) 535-2193
[email protected]
Florida
Mary Mattox
US EPA Region IV MC: 9T25
61 Forsyth Street, S.W.
Atlanta, GA 30303-8960
(404) 562-9733
[email protected]
Georgia
Emily Wingo
Georgia DNR
Environmental Protection Division
Watershed Protection Branch
4220 International Parkway, Suite 101
Atlanta, GA 30354
(404) 362-2680
[email protected]
Guam
Maricar Quezon
Water Pollution Control Program
Guam EPA
P.O. Box 22439
Barrigada, Guam 96921
(671) 475-1601
[email protected]
Hawaii
Scott Miyashiro
Hawaii Department of Health
Clean Water Branch
919 Ala Moana Boulevard, Room 301
Honolulu, HI 96814-4920
(808) 586-4309
[email protected]

Idaho
Robert Grandinetti
U.S. EPA Region X
NPDES Compliance Unit
309 Bradley Boulevard, Suite 115
Richland, WA 99352
(509) 376-3748
[email protected]

Maine
Richard E. Darling
Maine DEP
Division of Water Quality Management
17 State House Station
Augusta, ME 04333-0017
(207) 287-7806
[email protected]

Illinois
Michelle Rousey
Illinois EPA
Bureau of Water
1021 North Grand Avenue East
P.O. Box 19276 (Mail Code 15)
Springfield, IL 62794-9276
(217) 785-3944
[email protected]

Maryland
Ron Wicks
Maryland Dept. of the Environment
Water Management Administration
Compliance Program
1800 Washington Blvd., Suite 420
Baltimore, MD 21230
(410) 537-3607
[email protected]

Indiana
Becky Ruark
Indiana DEM/OWQ
100 North Senate Avenue
MC 65-42 IGCN N1255
Indianapolis, IN 46204-2251
(317) 233-2477
[email protected]

Massachusetts
Ping Yee
Massachusetts DEP/Boston Office
1 Winter Street, 6th Floor
Boston, MA 02108
(617) 292-5769
[email protected]

Iowa
Robert Bryant
U.S. EPA Region VII - Water Enforcement
11201 Renner Blvd
Lenexa, KS 66219
(913) 551-7354
[email protected]
Kansas*
N. Myron Gunsalus, Jr.
Kansas Dept of Health & Environment
Kansas Health and Environmental
Laboratories
Forbes Field Building 740
Topeka, KS 66620-0001
(785) 291-3162
[email protected]

Michigan
Chris Veldkamp
Michigan Dept of Environmental Quality
Water Resources Division
State of Michigan Building
350 Ottawa Ave NW Unit 10
Grand Rapids, MI 49503
(616) 356-0263
[email protected]
Minnesota
Roger Fisher
Minnesota Pollution Control Agency
Environmental Outcomes Division/EMAS
520 Lafayette Road
St. Paul, MN 55155
(651) 757-2360
[email protected]

Kentucky
Abigail Rains
Kentucky DEP
Surface Water Permits Branch
200 Fair Oaks Lane, 4th Floor
Frankfort, KY 40601
(502) 564-3410 x4891
[email protected]

Mississippi
David Singleton
Mississippi DEQ
Office of Pollution Control
1542 Old Whitfield Road
Pearl, MS 39208
(601) 961-5778
[email protected]

Louisiana*
Sandy Bateman
Louisiana DEQ
Office of Environmental Compliance
P.O. Box 4312
Baton Rouge, LA 70821-4312
(225) 219-3614
[email protected]

Missouri
Robert Bryant
U.S. EPA Region VII - Water Enforcement
11201 Renner Blvd
Lenexa, KS 66219
(913) 551-7354
[email protected]

Colorado
Eric Mink
Colorado Dept. of Public Health and
Environment
Water Quality Control Division-CWE-B2
4300 S Cherry Creek Drive South
Denver, CO 80246
303-692-2312
[email protected]

Page 7

Montana
Lisa Tucker
Montana DEQ
Water Protection Bureau
P.O. Box 200901
Helena, MT 59620-0901
(406) 444-5388
[email protected]

Northern Mariana Islands
Clarissa T. Bearden
Laboratory Director
CNMI Division of Environmental Quality
P.O. Box 501304
Saipan, MP 96950
(670) 664-8520
[email protected]

Nebraska
Reuel Anderson
Nebraska DEQ
Water Quality Division
P.O. Box 98922
Lincoln, NE 68509
(402) 471-1367
[email protected]

Liliana Christophe
U.S. EPA Region IX
75 Hawthorne Street
Mail Code WTR 7
San Francisco, CA 94105
(415) 972-3502
[email protected]

New Hampshire
Stephanie Larson
New Hampshire DES - Water Division
P.O. Box 95
Concord, NH 03302-0095
(603) 271-1493
[email protected]
New Mexico
Barbara Cooney
New Mexico Environment Department
Surface Water Quality Bureau
Harold Runnels Bldg, N 2050
1190 South St. Francis Drive (8750S)
P.O. Box 5469
Santa Fe, NM 87505-5469
(505) 827-0212
[email protected]
New York
Towana Joseph
US EPA Region II, DESA/MAB
2890 Woodbridge Avenue (MS 220)
Edison, NJ 08837
(732) 321-6607
[email protected]
North Dakota
Marty Haroldson
North Dakota Dept of Health
Division of Water Quality
Permits Program
918 East Divide Ave., 4th Floor
Bismarck, ND 58501
(701) 328-5234
[email protected]

Ohio
Steve Roberts
Ohio EPA
8955 East Main Street
Reynoldsburg, OH 43068
(614) 644-4225
[email protected]
Oklahoma*
David Caldwell
Oklahoma DEQ
P.O. Box 1677
Oklahoma City, OK 73101
(405) 702-1039
[email protected]
Angela Ratcliff
Oklahoma DEQ
P.O. Box 1677
Oklahoma City, OK 73101
(405) 702-8146
[email protected]
Oregon
Chris Redman
Oregon DEQ
Laboratory& Environmental
Assurance Division
3150 NW 229th Ave Suite 150
Hillsboro, OR 97124
(503) 693-5706
[email protected]

Puerto Rico
Towana Joseph
US EPA Region II, DESA/MAB
2890 Woodbridge Avenue (MS 220)
Edison, NJ 08837
(732) 321-6607
[email protected]

Vermont
Andrew Fish
Vermont ANR - DEC / WWMD
103 S. Main Street, Sewing Building
Waterbury, VT 05671
(802) 241-3739
[email protected]

Rhode Island
Alexandre Pinto
Rhode Island DEM
Office of Water Resources
235 Promenade Street
Providence, RI 02908
(401) 222-4700 x7227
[email protected]

Virgin Islands
Towana Joseph
US EPA Region II, DESA/MAB
2890 Woodbridge Avenue (MS 220)
Edison, NJ 08837
(732) 321-6607
[email protected]

South Dakota
Tim Flor
South Dakota Dept of Environment &
Natural Resources
Surface Water Quality Program
PMB 2020
523 East Capital
Pierre, SD 57501
(605) 773-3351
[email protected]
Tennessee
Dana Waits
Division of Water Resources
Compliance & Enforcement Unit
William R. Snodgrass TN Tower
312 Rosa Parks Blvd, 11th Floor
Nashville, TN 37243
(615) 532-1171
[email protected]
Texas
N.D. Kaku
Texas Commission on Environmental Quality
P.O. Box 13087 (MC 165)
Austin, TX 78711
(512) 239-6684
[email protected]

Utah*
Monia Ben-Khaled
US EPA Region VIII
NPDES Enforcement Unit
Water Technical Enforcement Program
Pennsylvania*
Department of Environmental Protection 1595 Wynkoop Street (8ENF-W-NP)
Denver, CO 80202
Bureau of Laboratories
(303) 312-6209
Attn: Laboratory Accreditation Program
[email protected]
P.O. Box 1467
Harrisburg, PA 17105-1467
[email protected]

Virginia*
DCLS Laboratory Certification Office
600 N. 5th Street
Richmond, VA 23219
(804) 648-4480
[email protected]
Washington
Alan Rue
Washington State Dept of Ecology
Laboratory Accreditation Unit
P.O. Box 488
2350 Colchester Drive
Manchester, WA 98353
(360) 895-6178
[email protected]
West Virginia*
Linda B. Keller
601 57th Street, S.E.
Charleston, WV 26304
(304) 926-0495
[email protected]
Wyoming
Edward Mock
Wyoming DEQ
Water Quality Division
122 W. 25th Street
Cheyenne, WY 82002
(307) 777-7317
[email protected]

*Some or all permittees in these states may not be required to participate in DMR-QA due to a full or partial waiver agreement with EPA.

Your coordinator may change. Please consult: http://www.epa.gov/compliance/contact/monitoring-dmr-state.html
and http://www.epa.gov/compliance/contact/monitoring-dmr.html for the latest list of coordinators.
For additional questions, contact US EPA Headquarters:
Brian Krausz
USEPA/MAMPD (2227A)
1200 Pennsylvania Ave, NW
Washington, DC 20460
(202) 564-3069
[email protected]
Page 8

OMB Control No. 2080-0021 Approval expires 1/31/2014

United States

ENVIRONMENTAL PROTECTION AGENCY
Washington, DC 20460
Laboratory DMR-QA Evaluation Study 34
Laboratory Performance Evaluation
Office of Enforcement and Compliance Assurance
(This data is collected under the authority of Section 308 of the Clean Water Act.)

Paperwork Reduction Act Notice
The public reporting and recordkeeping burden for this collection of information is estimated to average 6.3 hours per response. Send
comments on the Agency's need for this information, the accuracy of the provided burden estimates, and any suggested methods for
minimizing respondent burden, including through the use of automated collection techniques to the Director, Collection Strategies
Division, U.S. Environmental Protection Agency (2822T), 1200 Pennsylvania Ave., NW, Washington, DC 20460. Include the OMB control
number in any correspondence. Do not send the completed form to this address.

Instructions for the NPDES Permittee Data Report Form
1.

This is a two-page form.

2.

Enter your NPDES permit number at the top of pages 10 and 11.

3.

You must fill in the 2-digit permit extension field at the top of page 10 if there is an extension for your
permit code. If you have one, the extension will appear next to your permit code in the address box on
page 11; for example: "NPDES Permittee CA1234567-01." If there is no extension, leave this field blank.

4.

Identify each of your laboratories on page 11, including their U.S. EPA Lab code which is a unique
identifier number assigned by EPA. (Refer to page 3, item 4 in the Study 34 packet.) (NOTE: The U.S.
EPA lab code of the laboratory that produced the data must also appear at the top of the Chemistry/
Microbiology and WET testing checklists on pages 12-13.)

5.

Make copies of pages 10 and 11. Then attach a copy of these pages to the Chemistry/Microbiology and
WET testing checklists. Separate copies of each checklist must be filled out for each laboratory you
used. Also, if a laboratory reports more than one method to you for any single analyte, you must use a
separate checklist for each method reported. These checklists must indicate the graded results for the
analytes tested by the laboratory that are in your permit and required for DMR-QA (Acceptable or Not
Acceptable). New for Study 34, it is optional but encouraged to write in the approved test method used
for DMR-QA Chemistry/Microbiology analytes. If you use a state-certified laboratory to generate your
NPDES data in a state that has been granted a partial exemption from the DMR-QA study by the EPA
Region, check the "Analyte determined by state-certified laboratory" box(es) on the checklists (pages 12
and 13) for all analytes in your permit analyzed by a certified laboratory.

6.

Sign and date the certification statement on page 10.

7.

Make copies of the NPDES Permittee Data Report form and checklists for your records.

8.

Submit the original signed copy of the Permittee Data Packages to the State DMR-QA coordinator no
later than August 29, 2014.

EPA 6400-01 Rev. 1-14 Previous editions are obsolete.

Page 9

OMB Control No. 2080-0021 Approval expires 1/31/2014

United States

ENVIRONMENTAL PROTECTION AGENCY
Washington, DC 20460
Laboratory DMR-QA Evaluation Study 34
Laboratory Performance Evaluation
Office of Enforcement and Compliance Assurance
(This data is collected under the authority of Section 308 of the Clean Water Act.)

NPDES Permittee Data Report Form

Due August
29, 2014

Attention: Follow the instructions on the previous page to complete this form and submit data for evaluation.
NPDES Permit Number

State

Permit Extension

Permittee Name
Current Permittee Mailing Address
City

State

Phone Number

Fax Number

Zip Code

E-mail

Optional: If WP Study was used, list PT Provider name(s):

Optional: WP Study Number(s)

For DMR-QA Study 34, conducted in 2014, the Permittee ensured that their laboratory(s) performing the required analyses:
Received PT Samples
Yes

Submitted Complete and Accurate Data by July 11, 2014

No

Yes

Received a Graded Report by August 1, 2014

No

Yes

No

Certification by Permit Holder or Authorized Representative
(as per 40 C.F.R. Section 122.22 - see instructions.)

I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system
designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons
who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my
knowledge and belief, true, accurate, and complete. Each reported value was produced from a single analytical run using the analytical system that
routinely performs these analyses to produce compliance monitoring data required under our National Pollutant Discharge Elimination System
(NPDES) permit. Neither I nor any of my subordinates compared our results with results from independent analyses conducted by us or any other
laboratory before we reported our results to the U.S.EPA. I am aware that there are significant penalties for submitting false information, including
the possibility of fine and imprisonment for knowing violations.

Name of Certifying Official

Title

Signature

Date

Address, phone number and e-mail of certifying official are required if different from above.

Address
City

EPA 6400-01 Rev. 1-14. Previous editions are obsolete.

Phone Number
State

Zip Code

Page 10

E-mail

OMB Control No. 2080-0021 Approval expires 1/31/2014

United States

ENVIRONMENTAL PROTECTION AGENCY
Washington, DC 20460
Laboratory DMR-QA Evaluation Study 34
Laboratory Performance Evaluation
Office of Enforcement and Compliance Assurance
(This data is collected under the authority of Section 308 of the Clean Water Act.)

Permittee Name

State

NPDES Permit No.

Permit Extension

Identification of all CHEM, MICRO and WET laboratories who performed analyses for this permit
Name of Laboratory

Address of Laboratory

U.S. EPA Lab
Code

Lab Analysis
Check box(es) that apply

Chem Micro

WET

Lab
Type*

Statecertified
Lab**

* Lab Types: C = Commercial F = Federal G = Local Government I = Industrial O = Other S = State
** See Footnote on page 2 (Frequently Asked Questions) for the current list of states with lab accreditation programs
If you need additional space, please make a copy of this page for additional laboratories.
EPA 6400-01 Rev. 1-14. Previous editions are obsolete.

Page 11

Permittee name

State

NPDES Permit No.

EPA Lab Code

Chemistry/Microbiology Analyte Checklist
DMR-QA Study 34

Laboratory's Graded Result

Microbiology

Analyte Test

Test Required

Method Number
Used (optional)

Acceptable

Not Acceptable
(Corrective Action Required)

Analyte determined by
state-certified lab*

E. coli.
Fecal Coliform, MF or MPN
Total Coliform, MF or MPN
Trace Metals

Aluminum
Antimony
Arsenic
Barium
Beryllium
Cadmium
Chromium, total
Chromium, hexavalent
Cobalt
Copper
Iron
Lead
Manganese
Mercury
Mercury (Low Level)
Molybdenum
Nickel
Selenium
Silver
Thallium
Vanadium
Zinc
Demands

5-day BOD
5-day Carbonaceous BOD
COD
TOC
Minerals

Alkalinity, total (CaCO3)
Chloride
Fluoride
Hardness, total (CaCO3)
Specific conductance (25°C)
Sulfate
Total Dissolved Solids (180°C)
Nutrients

Ammonia as N
Nitrate as N
Nitrite as N
Orthophosphate as P
Total Kjeldahl-Nitrogen as N
Total Phosphorus as P
Misc. Analytes

Non-Filterable Residue (TSS)
Oil and Grease
pH
Total Cyanide
Total Phenolics (4-AAP)
Total Residual Chlorine
Total Residual Chlorine (Low Level)
Settleable Solids
Turbidity

Name
** See Footnote on page 2

Signature
Page 12

Date
Use a separate checklist for EACH lab used

Permittee name

State

NPDES Permit No.

EPA Lab Code

WET Organisms/Test Conditions/End Points Checklist
DMR-QA Study 34

Laboratory's Graded Result
Analyte
Number

Organisms / Conditions

End Points

Test
Required

Acceptable

Analyte
determined by
Not Acceptable (Corrective state-certified
Action Required)
Lab*

Test Code 13/EPA Method 2000
754

Fathead minnow (Pimephales promelas) - MHSF 25°C

LC50

Test Code 14/EPA Method 2000
755

Fathead minnow (Pimephales promelas) - 20% DMW

LC50

Test Code 15/EPA Method 1000
808

Fathead minnow (Pimephales promelas) - MHSF

IC25** (ON) GROWTH

810

Fathead minnow (Pimephales promelas) - MHSF

NOEC (ON) GROWTH

Test Code 16/EPA Method 1000
812

Fathead minnow (Pimephales promelas) - 20% DMW

IC25** (ON) GROWTH

814

Fathead minnow (Pimephales promelas) - 20% DMW

NOEC (ON) GROWTH

Test Code 19/EPA Method 2002
764

Ceriodaphnia dubia - MHSF 25°C

LC50

Test Code 20/EPA Method 2002
765

Ceriodaphnia dubia - 20% DMW 25°C

LC50

Test Code 21/EPA Method 1002
767

Ceriodaphnia dubia - MHSF

IC25** REPRODUCTION

768

Ceriodaphnia dubia - MHSF

NOEC REPRODUCTION

Test Code 22/EPA Method 1002
770

Ceriodaphnia dubia - 20% DMW

IC25** REPRODUCTION

771

Ceriodaphnia dubia - 20% DMW

NOEC REPRODUCTION

Test Code 32/EPA Method 2021
788

Daphnia magna - MHSF 25°C

LC50

Test Code 38/EPA Method 2021
794

Daphnia pulex - MHSF 25°C

LC50

Test Code 42/EPA Method 2007
798

Mysid (Mysidopsis bahia) 25°C

LC50

Test Code 43/EPA Method 1007
816

Mysid (Mysidopsis bahia)

IC25** (ON) GROWTH

818

Mysid (Mysidopsis bahia)

NOEC (ON) GROWTH

Test Code 44/EPA Method 2006
803

Inland silverside (Menidia berylina) 25°C

LC50

Test Code 45/EPA Method 1006
825

Inland silverside (Menidia berylina)

IC25** (ON) GROWTH

826

Inland silverside (Menidia berylina)

NOEC (ON) GROWTH

Test Code 46/EPA Method 2004
804

Sheepshead minnow (Cyprinodon variegatus) 25°C

LC50

Test Code 47/EPA Method 1004
820

Sheepshead minnow (Cyprinodon variegatus)

IC25** (ON) GROWTH

822

Sheepshead minnow (Cyprinodon variegatus)

NOEC (ON) GROWTH

Name

Signature

Date

*See Footnote on page 2.
**Preferred endpoint for DMR-QA performance test reporting

Page 13

Use a separate checklist for EACH lab used