Additional Biospecimen Informatiion

Population Assessment of Tobacco and Health (PATH) Study (NIDA)

Attachment 6
PATH Study
Additional Information on Biospecimens
June 26, 2014

Population Assessment of Tobacco and Health (PATH) Study (NIDA)

PATH Biospecimen Analytes by Specimen and Biomarker, Wave 2
Analyte
Preferred Matrix
Tobacco Biomarkers in Tobacco Users and Non-tobacco Users

Biomarker

Nicotine and nicotine metabolites
Tobacco user: cotinine and trans-3'hydroxycotinine
Non-tobacco user: cotinine

Serum

Exposure

Nicotine and nicotine metabolites
Tobacco user: cotinine, trans-3'-hydroxycotinine,
cotinine N-oxide, nicotine N-oxide, nornicotine,
norcotinine; analogues: anabasine, anatabine
Non-tobacco user: cotinine and trans-3'hydroxycotinine

Urine

Exposure

Tobacco specific nitrosamines (TSNAs): NNAL,
NNN, NNA, NAT, NAB, NNK

Urine

Exposure

Polycyclic aromatic hydrocarbons (pyrene,
naphthalene, phenanthrene, fluorene)

Urine

Combustion

Cadmium, cobalt, uranium, lead, strontium,
beryllium, manganese, and thallium

Urine, prescreened
container

Speciated arsenic (As III, As V, dimethylarsinic
acid (DMA), and monomethylarsonic acid (MMA))
Creatinine
4-ABP hemoglobin

Toxicity

Urine, prescreened
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Toxicity

Urine

For correction

Red blood cells, EDTA

Toxicity

VOCs metabolites

Urine

Combustion

Volatile nitrosamines

Urine

Combustion

Aromatic amines

Urine

Combustion

Cyanide

Urine

Combustion

Other Biomarkers in Tobacco Users and Non-tobacco Users
C-reactive protein

Serum, red top or SST

Fibrinogen
Interleukin 6
sICAM (soluble intercellular adhesion
molecule)
F2-isoprostane / 8-epi-prostaglandin F2a

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Inflammation;
Cardiovascular risk

Plasma, citrate

Cardiovascular risk

Plasma

Inflammation

Plasma, EDTA or
Serum, SST

Cardiovascular risk

Urine

Oxidative stress

Population Assessment of Tobacco and Health (PATH) Study (NIDA)

Note. At Wave 2, blood will be collected and analyzed from the adults who age into the adult
cohort from the youth cohort.
At Wave 2, urine will be collected and analyzed from a sample of 10,000 adults who also
provided urine and blood for analysis at baseline. In addition, urine will be collected and
analyzed from the adults who age into the adult cohort from the youth cohort.

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