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OMB Control #0693-0033
Expiration Date: 06/30/2019
1. Name of participant (optional):
* 2. Title/Position
* 3. Number of people in laboratory
5 or under
5 to 10
10 to 15
over 15
* 4. Type of institution (select those that apply)
Academia
Core Facility
Industry
Government
5. Laboratory PI name (if same as above, fill in see above)
* 6. Name of Institution
* 7. Location
Other
About the Laboratory
OMB Control #0693-0033
Expiration Date: 06/30/2019
* 8. How long has your laboratory been performing lipidomics?
< 1 year
1 to 5 years
5 to 10 years
> 10 years
* 9. Approximately how many lipid samples does your laboratory analyze in a month?
< 50
50 to 100
100 to 500
> 500
* 10. Approximately how many lipidomics manuscripts does your laboratory publish per year?
0
1 to 3
3 to 5
>5
* 11. What kind of lipid applications do you typically work on in your laboratory (select those that apply)?
Clinical and medical science
Toxicology
Environmental science
Biomarker discovery
Food science
Plant science
Drug development/discovery
Forensics
Natural products
Other (please specify)
* 12. What kind of sample matrices does your laboratory analyze for lipidomics (select those that apply)?
plasma
saliva/sweat/tears
serum
dried blood spots
urine
breast milk
tissues
food
cells
plant materials
feces
Other (please specify)
* 13. What strategies (if any) does your laboratory employ for enhancing/monitoring lipid stability (select
those that apply)?
antioxidant addition
sample preparation performed on ice
use of inhibitors
use of heat treatment
use of internal/recovery standards
derivatization
flash freezing
no specific strategies employed
Other (please specify)
* 14. What kind of separation technique does your laboratory use in tandem with mass spectrometry for
lipidomics (select those that apply)?
ion mobility
high performance liquid
chromatography
shotgun/direct infusion
direct analysis (ex., DART, DESI)
gas chromatography
ultra-high performance liquid
chromatography
Other (please specify)
15. If chromatography, what type of column(s) does your laboratory use for lipidomics?
* 16. What kind of instruments does your laboratory use for the above mentioned methods (select those that
apply)?
Orbitrap
Fourier Transform Ion Cyclotron
Resonance
Flame Ionization Detector
Quadrupole Time-of-Flight
Ion Trap
Triple Quadrupole
Nuclear Magnetic Resonance
Other (please specify)
* 17. What data acquisition methods does your laboratory incorporate for targeted studies (select those that
apply)?
neutral loss scans
single/selected reaction monitoring
parent ion scans
we only apply untargeted approaches
product ion scans
Other (please specify)
* 18. What data acquisition methods does your laboratory incorporate for untargeted studies (select those
that apply)?
accurate mass
data independent MS/MS
data dependent low-resolution MS/MS
we only employ targeted approaches
data dependent high-resolution MS/MS
Other (please specify)
* 19. If you incorporate a high-resolution mass spectrometer, at what mass resolving power do you analyze
your lipid extracts (answer N/A if you only use a low-resolution mass spectrometer)?
* 20. What lipid categories do you routinely measure in your laboratory (select those that apply)?
Fatty Acyl Lipids
Sphingolipids
Saccharolipids
Glycerolipids
Sterol Lipids
Polyketides
Glycerophospholipids
Prenol Lipids
Other (please specify)
* 21. For untargeted lipidomics experiments, what lipid extraction does your laboratory employ (select those
that apply)?
Bligh-Dyer
MTBE (Matyash)
Solid Phase Extraction
Folch
Supercritical Fluid Extraction
We do not perform untargeted
lipidomics experiments
Other (please specify)
* 22. If you use LC-MS, what software does your laboratory employ for peak picking/processing (select all
that apply)?
Manual (Xcalibur, MassHunter,
MassLynx, other)
MS-DIAL
Progenesis QI
Lipidyzer
Compound Discoverer
SimLipid
We do not use LC-MS
MZmine
XCMS
Sieve
LipidSearch
Other (please specify)
* 23. What software does your laboratory employ for lipid identification (select all that apply)?
Manual (visual inspection)
MS-LAMP
Greazy
LipidSearch
LIMSA
LipidBlast
Lipidyzer
LOBSTAHS
LipidPioneer
SimLipid
Lipid Data Analyzer
mzCloud
Alex
LipidQA
LipidMatch
LipidXplorer
Lipid-Pro
Other (please specify)
* 24. What lipid databases do you use (select those that apply)?
Japan’s LipidBank
LipidHome
LIPID MAPS
Cyberlipid
LipidBlast
SphinGOMAP
European Lipidomics Initiative
mzCloud
SwissLipids
NIST Mass Spectrometry Database
Other (please specify)
* 25. What software does your laboratory employ for lipid quantification (select those that apply)?
Manual
Sieve
LipidSearch
TraceFinder
Lipidyzer
Progenesis QI
SimLipid
Other (please specify)
* 26. What software does your laboratory employ for lipid quality control and statistics (select those that
apply)?
MetaboAnalyst
Orange
S-PLUS
SPSS
JMP
NCSS
Excel
Tableau
GraphPad Prism
R-tools
TraceFinder
Statistica
PLS_Toolbox
MATLAB
PSPP
Origin
Stata
Analyze-it
Galaxy toolbox
Minitab
SYSTAT
Other (please specify)
Lipid Quantitation
OMB Control #0693-0033
Expiration Date: 06/30/2019
* 27. What type of quantitation do you perform in your laboratory?
absolute
relative
semi-quant
not interested in quantitation of lipids
* 28. Is absolute quantitation something that could be important to your laboratory?
Yes
No
* 29. On average, how many lipid internal standards do you use per lipid class?
1
2
3 or more
* 30. What type of internal standards does your laboratory most often employ (select those that apply)?
odd-chain
deuterated
low fatty acyl carbon chain (12:0 or
less)
carbon-13 labeled
Isotopic Ratio Outlier Analysis
Other (please specify)
* 31. Do you make your own lipid internal standard mix or buy pre-made mixtures?
We make our own internal standard mixtures
We buy pre-made internal standard mixtures
We do both
* 32. What lipids do you find most challenging to quantitate (select those that apply)?
free/total fatty acids
sphingomyelins
phosphatidylethanolamines
cholesterol
eicosanoids
phosphatidylglycerols
cholesteryl esters
bile acids
phosphatidylinositols
triacylglycerols
lysophosphatidylcholines
phosphatidylserines
diacylglycerols
lysophosphatidylethanolamines
phosphatidic acids
ceramides
phosphatidylcholines
Other (please specify)
* 33. Does your laboratory employ relative response factors (RRFs) for these lipid categories (select those
that apply)?
Fatty acyl
lipids
Glycerolipids
Glycerophos
pholipids
Sphingolipids
Sterols
All of the
above
We do not
employ RRFs
Other (please specify)
* 34. How does your laboratory treat multiple adducts per lipid (select those that apply)?
sum them
use the most intense for each ionization
mode
average them
report individual adducts (no further
processing)
Other (please specify)
* 35. When processing lipid data, does your laboratory use peak height or peak area for quantitation?
peak height
peak area
* 36. How does your laboratory normalize your quantitative lipid values (select those that apply)?
total protein
dry weight
normalize by sum of feature values
DNA
wet weight
probabilistic quotient normalization
cell count
TIC
no normalization
Other (please specify)
Reference Material/Quality Control
OMB Control #0693-0033
Expiration Date: 06/30/2019
* 37. Do you have written standard operating procedures (SOPs) in your laboratory, and if so, what aspects
do the SOPs cover (select those that apply)?
instrument calibration/maintenance
data processing
sample collection
monitoring lipid stability
sample extraction
assessment of data quality/quality control
sample storage
we do not have SOPs in our laboratory
instrument operation
Other (please specify)
* 38. Has your laboratory adopted the proposed shorthand annotation style for lipid structures at the fatty
acyl level (doi:10.1194/jlr.M033506), when the sn1 and sn2 position of the fatty acyl chains is unknown
(e.g., PC 16:0_18:1)?
Yes
No
* 39. What types of quality control (QC) samples does your laboratory use in analytical measurements for
lipidomics?
no QC materials
pooled samples (matrix-matched)
solvent blanks
pooled samples (not matrix-matched)
extraction blanks
NIST Standard Reference Materials
(SRMs)
Certified Reference Materials (CRMs)
Other (please specify)
40. For question 35, state whether the QC material you employ is commercially available or made in-house.
For commercially available answers, please specify the material name.
* 41. What does your laboratory use QCs, SRMs, or CRMs for (select those that apply)?
Establishing metrological traceability
Method validation (method variance)
Technical variance
Calibration
Establish trueness of result
We don't use QCs, SRMs, or CRMs
Value assignment of secondary reference material
Other (please specify)
* 42. If your laboratory uses commercially available QC materials (ex. NIST SRMs), please indicate below;
however, if your laboratory does not use commercially available reference materials, indicate why below?
we use commercially
available QCs
don't know about
them
don’t see value
too expensive
correct matrix not
available
Other (please specify)
* 43. What type of reference material would be of most interest to your laboratory?
Complex biological matrix
Lipid internal standard mixture
Lipid standard mixture
Spiked standards in a complex biological matrix
* 44. What types of complex biological reference materials would you like to see provided?
plasma
cells
breast milk
serum
feces
food
urine
saliva/sweat/tears
plant materials
tissues
dried blood spots
not interested in reference materials
Other (please specify)
* 45. Do you validate your project sample measurements with:
repeated extractions of a sample (with analysis)
reviewing measurements of a previously described quality
control sample run in the same batch
repeated instrument analysis of a sample
test set
sent to outside laboratory
no validation process employed
use a complimentary approach to confirm
Other (please specify)
* 46. About how long does your laboratory store extracted lipidomics samples before you discard?
Less than a day
One month to less than 6 months
One day to less than a week
6 months to a year
One week to less than a month
Greater than a year
47. What temperature(s) does your laboratory store lipid extracts at (select those that apply)?
room temperature
freezer (-80 C)
refrigerated (2-4 C)
liquid nitrogen
freezer (-20 C)
Other (please specify)
* 48. Does your laboratory store your lipid data in a repository? If yes, where?
Other Questions
OMB Control #0693-0033
Expiration Date: 06/30/2019
* 49. What do you perceive as the biggest challenge in the lipidomics community (select those that apply)?
lack of standardization of methods/protocols within the
community
quantitation
over reporting/false positives
lack of standards
lack of lipid centric training/workshops
software/data handling
lipid annotation
Other (please specify)
* 50. Has your laboratory ever participated in an interlaboratory comparison study or ring trial?
Yes
No
* 51. Would your laboratory be interested in participating in a future NIST interlaboratory study?
Yes
No
* 52. Do you feel there are enough opportunities and/or lipidomics conferences per year to present lipidomics
studies?
Yes
No
* 53. Would you be interested in attending or presenting at a Gordon Research Conference focused on the
measurement science of lipidomics and metabolomics?
Attending
Presenting
No interest
54. Additional comments?
Notwithstanding Statement
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person is required to respond to, nor shall any person be subject to a penalty for failure to comply
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National Institute of Standards and Technology, Attn: John A. Bowden ([email protected]).
OMB Control No. 0693-0033
Expiration Date: 06/30/2019
| File Type | application/pdf |
| File Title | View Survey |
| File Modified | 2017-03-21 |
| File Created | 2017-03-13 |