Attachment C Work with Animals

This submission is:

A new registration

An update to an existing registration

A renewal

Date:

Entity Name:
Laboratory Safety Level:
PI(s):

Attachment C - Work with Animals
1. Provide the select agent/toxin and species of animal to be used:

Select Agent/Toxin

Species of Animal

Route(s) of Administration
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2. Are animals exposed to select agents or toxins by the aerosol route? ........................................................................ Yes

No

If yes, is the aerosol exposure equipment used within a primary containment device? ....................................... Yes

No

3. Is the entity waste stream treated prior to disposal? .......................................................................................... Yes

No

If no, explain ____________________________________________________________
If yes,
a. What is the method of treating carcasses?
Incineration
Tissue Digester
Autoclaved
Describe validation procedures that account for variables such as time and temperature of autoclave run cycles,
as well as temperature and weight of carcass at initiation of autoclave cycle.

Chemical (disinfectant, concentration, and time)

Other

b. What is the method of treating infectious waste (e.g., cell cultures, infected arthropods, other biohazardous waste)?

Incineration
Tissue Digester
Autoclaved
Describe validation procedures that account for variables such as time and temperature of autoclave run cycles,
as well as temperature and weight of carcass at initiation of autoclave cycle.

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Chemical (disinfectant, concentration, and time)

Other

c. What is the method of treating disposable material (e.g. PPE)?
Incineration
Tissue Digester
Autoclaved
Describe validation procedures that account for variables such as time and temperature of autoclave run cycles,
as well as temperature and weight of carcass at initiation of autoclave cycle.

Chemical (disinfectant, concentration, and time)

Other

d. What is the method of treating disposable caging (e.g. rodent/mosquito caging)?
Incineration
Tissue Digester
Autoclaved
Describe validation procedures that account for variables such as time and temperature of autoclave run cycles,
as well as temperature and weight of carcass at initiation of autoclave cycle.

Chemical (disinfectant, concentration, and time)

Other

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This submission is:

A new registration

An update to an existing registration

A renewal

Date:

Entity Name:
Laboratory Safety Level:
PI(s):

Attachment C - Work with Animals
4.

Are samples (tissue, blood, nucleic acids, etc.) from exposed or infected animals manipulated at a lower biosafety level?
If answer yes, follow up question:
Describe the inactivation process (e.g. formalin fixation, lysis of cells for nucleic acid extraction, irradiation) for the samples.
Include concentration or dosage and contact/exposure time, as applicable.

5. The entity requires that an Institutional Animal Care and Use Committee (IACUC) review and approve
protocols prior to work with animals at this entity. ........................................................................................................ Yes

No

If yes, the proposed work with the select agents and toxins in animals has been approved by the IACUC. ....... Yes

No

If no, explain.

6. The laboratory is accredited by the Association for Assessment and Accreditation of Laboratory Animal Care
(AAALAC). ................................................................................................................................................................... Yes
If yes, give the most recent (re)accreditation date

No

Date:
7.

There is a system in place for recording the number of animals infected, the number of animals disposed of,
and the records are reviewed frequently. .................................................................................................................... Yes
If yes, describe.

No

8. Are animals restrained for experimental manipulation? ................................................................................................ Yes
If no, explain.

No

9. Are animals intentionally or accidentally infected with select agents monitored (e.g. daily checks)?............................. Yes
If no, explain.

No

10. Describe animal housing for each species, including whether cages provide primary containment and a brief description
(e.g. cage or cage rack is HEPA filtered, active or passive ventilation of the cages, non-containment caging housed within
inward flow ventilated enclosure).

Species

Animal Housing
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This submission is:

A new registration

An update to an existing registration

A renewal

Date:

Entity Name:
Laboratory Safety Level:
PI(s):

Attachment C - Work with Animals
11. Are animals euthanized? ................................................................................................................................................ Yes
If no, explain.

No

12. Will animals be necropsied? .......................................................................................................................................... Yes

No

If yes, describe necropsy procedures.

13. Describe how animal carcasses are secured prior to decontamination.
Locked freezers, coolers
Not secured, immediately decontaminated (e.g.,autoclave, tissue digester, incinerator)
Other

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