Attachment E Work with Arthropods

A new registration

This submission is:

An update to an existing registration

A renewal

Date:

Entity Name:
Laboratory Safety Level:
PI(s):

Attachment E - Work with Arthropods
1. Work is performed with field-collected arthropods in a diagnostic capacity only for identification of
select agents. .............................................................................................................................................................. Yes

No

2. Work is performed to experimentally inoculate or infect arthropods (any stages) with select agents. ........................ Yes

No

If yes, complete questions 3-16.
3. Provide the select agent and species of arthropod used:

Select Agent

Species of Arthropod
Delete
Add Row

4. Arthropod experimental exposure route(s).
a. Injected with select agent. ............................................................................................................................... Yes

No

b. Infected with select agent via blood meal. ...................................................................................................... Yes

No

If yes, indicate the blood meal source.
Animal species

If vertebrate hosts are used, has the IACUC approved the work proposed in this objective
of work? ..................................................................................................................................... Yes

No

If yes, complete Attachment C - Work with Animals.
If no, explain.

Collected blood (describe type/method)

c. Infected with select agent via insect feeding on select agent infected plants. ................................................ Yes
If yes, complete Attachment D - Work with Plants.
d. Other (Describe)

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No

A new registration

This submission is:

An update to an existing registration

A renewal

Date:

Entity Name:
Laboratory Safety Level:
PI(s):

Attachment E - Work with Arthropods
5. Provide a description of the procedures used for primary containment and any transfer(s) of infected arthropods.

6. There is a system in place for recording the number of arthropods infected and the number of arthropods
disposed of, and the records are reviewed frequently.................................................................................................. Yes
If yes, describe.

No

7. Arthropod containment laboratory design and operational procedures are developed and implemented in
accordance with guidance found in the current edition of the Arthropod Containment Guidelines, a project of the
American Committee of Medical Entomology of the American Society of Tropical Medicine and Hygiene. ............... Yes

No

8. An Institutional Biosafety Committee (IBC) reviews and approves arthropod work with select agents at this facility. .. Yes

No

If yes,
a. has the IBC approved the arthropod containment laboratory design and operational procedures? ............... Yes

No

b. has the IBC approved the work described in this objective of work? .............................................................. Yes

No

If no, explain.

9. Are arthropods, including those experimentally infected, housed and manipulated in a suite/room such that accidental
contact and release is prevented?
Yes
10. Do protocols account for accidental escape? ............................................................................................................... Yes

No
No

11. Ventilation filters/barriers are installed to prevent arthropod escape. .......................................................................... Yes

No

12. Floor drains are present in the laboratory. .................................................................................................................. Yes

No

If yes, floor drains are modified to prevent accidental release of arthropods and agents. .................................

Yes

No

13. Suite/room plumbing is suitable to prevent arthropod escape. ................................................................................... Yes

No

14. All stages of arthropods are killed before disposal. .................................................................................................... Yes

No

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This submission is:

A new registration

An update to an existing registration

A renewal

Date:

Entity Name:
Laboratory Safety Level:
PI(s):

Attachment E - Work with Arthropods
15. All wastes from the arthropod containment laboratory are treated for disposal using an approved method. .............. Yes

No

If yes, describe method:
Autoclaved
Chemical (disinfectant, concentration, and time)

Incineration
Other

16. Animals or plants are permitted in the arthropod containment laboratory. .................................................................. Yes

No

If yes,
a. are animals or plants associated with the work being performed? ............................................................... Yes

No

b. are animals or plants accessible to escaped arthropods? ............................................................................ Yes

No

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