Attachment F BSL3Ag Laboratories

This submission is:

 A new registration

 An update to an existing registration

Entity Name:

 A renewal
Date:

PI(s):

Attachment F – BSL3Ag Laboratories
1.

Supplies, material and equipment enter and exit BSL3Ag areas only through an airlock,
fumigation chamber, an interlocked and double-door autoclave, or shower.
For materials which are temperature sensitive, a gas sterilizer, pass-through liquid
dunk tank, or a cold gas decontamination chamber are provided.

Yes

No

Yes

No

2.

Is a shower required when leaving the containment boundary

Yes

No

3.

Disposable materials are decontaminated by a verified method (check all that apply):
Autoclaved
Chemical (disinfectant, concentration, and time) _____________________________
Incineration __________________________________________________________
Other _______________________________________________________________

Yes

No

4.

All containment areas are designed, constructed and verified to function as a primary
containment barrier. All walls are constructed slab-to-slab and walls, floors, and ceilings
are sealed. All penetrations into the laboratory are sealed airtight to prevent escape of
agents and to allow fumigation for biological decontamination.

Yes

No

5.

Differential pressures/directional airflow are monitored and alarmed to indicate system
failure.

Yes

No

6.

There is HEPA filtration of all supply and exhaust air to and from the containment space.
If yes, all HEPA filters are certified annually.

Yes
Yes

No
No

7.

Laboratory procedure and design features include:
Personnel ingress and egress only through a series of rooms which includes a
a.
ventilated vestibule.
A clean change room outside of containment.
b.
Doors that define a containment boundary have compressible or inflatable gaskets
c.
with airtight hinges and latch/knob areas.
A shower room at the non-containment/containment boundary.
d.
A dirty change room within containment.
e.

Yes

No

Yes
Yes

No
No

Yes
Yes

No
No

Yes

No

8.

A second shower is required at the facility access control point before donning street
clothing.

This submission is:

 A new registration

 An update to an existing registration

Entity Name:

 A renewal
Date:

PI(s):

Attachment F – BSL3Ag Laboratories (Continued)
9.

Humane restraining devices are provided in large animal rooms.
If yes, describe. Add additional sheets as needed.

Yes

No

10.

Are necropsy rooms sized and equipped appropriately to accommodate animals?
If yes, describe. Add additional sheets as needed.

Yes

No

2

This submission is:

 A new registration

 An update to an existing registration

Entity Name:

 A renewal
Date:

PI(s):

Attachment G – BSL4/ABSL4 Laboratories
BSL4 LABORATORY
1.

Will work be performed in a BSL4/ABSL4 Cabinet Laboratory?
If yes, complete questions 2 - 8

2.

Describe the type of personal protective equipment that will be used. Add additional sheets as needed.

3.

Describe the decontamination methods for materials/equipment in the Class III cabinet. Add additional sheets
as needed.

4.

Describe what liquid effluents are decontaminated and how they are decontaminated. Add additional sheets
as needed.

5.

Describe the supply and exhaust components of the ventilation system, including how the ventilation system
of the Class III cabinet is manifolded to the room ventilation. Add additional sheets as needed.

6.

In the event of a ventilation failure, describe what measures are used to prevent reversal of airflow. Add
additional sheets as needed.

7.

Describe how differential pressures and directional airflow are monitored and analyzed. Add additional
sheets as needed.

8.

Describe how containment parameters are monitored daily. Add additional sheets as needed.

9.

Will work be performed in a BSL4/ABSL4 Suit Laboratory?
If yes, complete questions 10 - 16

10.

Describe the type of personal protective equipment that will be used. Add additional sheets as needed.

11.

Describe what liquid effluents are decontaminated and what measures are used to do so. Add additional
sheets as needed.

3

Yes

Yes

No

No

This submission is:

 A new registration

 An update to an existing registration

Entity Name:

 A renewal
Date:

PI(s):

Attachment G – BSL4/ABSL4 Laboratories (Continued)
12.

Describe the supply and exhaust components of the ventilation system, including how negative pressure is
maintained and HEPA filtration of supply and exhaust air. Add additional sheets as needed.

13.

In the event of a ventilation failure, describe what measures are used to prevent reversal of airflow. Add
additional sheets as needed.

14.

Describe how differential pressures and directional airflow are monitored and analyzed. Add additional
sheets as needed.

15.

In the event of a breathing air failure, describe what facility redundancies are in place. Add additional sheets
as needed.

16.

Describe how containment parameters are monitored daily. Add additional sheets as needed.

4