Form 0920-0978 FluSurv NET Lab Survey

Testing Facility Name:

Testing Facility ID (FluSurv-NET use only):

Name of person completing form:

Date:

U.S. DEPARTMENT OF
HEALTH AND HUMAN SERVICES
CENTERS FOR DISEASE CONTROL
AND PREVENTION
ATLANTA, GA 30329

FluSurv-NET Laboratory Survey 2017–2018 Season
Form Approved
OMB No. 0920-0978

Survey Introduction

Administer this survey to labs that serve FluSurv-NET hospitals. The questions in this survey refer to diagnostic testing ordered by healthcare
providers for routine clinical care of hospitalized and emergency department (ED) patients only. All questions relate to testing performed on-site within
the lab facility unless otherwise specified. If a FluSurv-NET hospital lab sends specimens to one or more labs (other than commercial or state public
health labs) for clinical influenza or RSV testing, please have each lab complete this survey.
• Do NOT administer this survey to commercial labs or to state public health labs
• Do NOT administer this survey to labs that are not affiliated with FluSurv-NET hospitals
• Do NOT include information on testing for the purposes of influenza or RSV surveillance
• Do NOT include information on testing for outpatients
This survey should take 10-15 minutes to complete. Thank you for your time!

Question
1. What is the role of the person completing this survey?
Laboratory staff at testing facility

FluSurv-NET staff

2. Please select the choice which best describes the laboratory type: (select one)
Hospital (private/public/community) laboratory

County public health laboratory

Federal government (military, IHS, Veteran’s Affairs) hospital laboratory

Other (specify):

University/medical school hospital laboratory

Influenza
3. Does the laboratory perform diagnostic testing for influenza on-site?
Yes  Answer question 4

No  Skip to Question 10

4. Does the laboratory perform rapid influenza antigen diagnostic test (rapid test, RIDT)?
Yes, pediatric patients only  Answer questions 4a-4d
Yes, adult patients only  Answer questions 4a-4d

No, we confirm RIDT tests performed elsewhere in the hospital (such as ED)
 Answer questions 4c-4d

Yes, pediatric and adult patients  Answer questions 4a-4d

No  Skip to question 5

4a. Select the kit name(s) (manufacturer) for the rapid influenza antigen diagnostic test(s) performed at the laboratory: (Check all that apply)
(http://www.cdc.gov/flu/professionals/diagnosis/rapidclin.htm)
BD Directigen™ EZ Flu A+B (Becton-Dickinson & Co.)

QuickVue® Influenza A+B Test (Quidel Corp.)

BD Veritor™ System for Rapid Detection of Flu A+B (CLIA-waived), (Becton
Dickinson & Co.)

RAMP Influenza A/B Assay or 3M™ Rapid Detection Flu A+B Test
(Response Biomedical Corp.)

BD Veritor™ System for Rapid Detection of Flu A+B (Moderately Complex),
(Becton Dickinson & Co.)

SAS™ FluAlert A&B Test (SA Scientific, Inc.)

Binax NOW® Influenza A&B Test (Alere Scarborough, Inc.)

SAS™ Influenza B Test (SA Scientific, Inc.)

BioSign® Flu A+B or OraSure QuickFlu Rapid A+B Test or Polymedco Poly stat
Flu A&B Test or LifeSign LLC Status Flu A&B (Princeton BioMedtech Corp.)

Sofia® Analyzer and Influenza A+B FIA (CLIA-waived) (Quidel Corp.)

ClearView Exact II Influenza A&B Test or Alere Influenza A&B Test (Alere
Scarborough, Inc.)

TRU FLU® (Meridian Bioscience, Inc.)

OSOM® Influenza A&B Test (Sekisui Diagnostics)
QuickVue® Influenza A/B Test (Quidel Corp.)

SAS™ Influenza A Test (SA Scientific, Inc.)

Sofia® Analyzer and Influenza A+B FIA (Quidel Corp.)
XPECT™ Influenza A/B (Remel Inc./Thermo Fisher Scientific)
Other, specify:

4b. If more than one kit is selected above, please select the one kit that is (or will be) used most frequently for rapid influenza diagnostic testing at the
laboratory during the current influenza season:
BD Directigen™ EZ Flu A+B (Becton-Dickinson & Co.)

QuickVue® Influenza A+B Test (Quidel Corp.)

BD Veritor™ System for Rapid Detection of Flu A+B (CLIA-waived), (Becton
Dickinson & Co.)

RAMP Influenza A/B Assay or 3M™ Rapid Detection Flu A+B Test
(Response Biomedical Corp.)

BD Veritor™ System for Rapid Detection of Flu A+B (Moderately Complex),
(Becton Dickinson & Co.)

SAS™ FluAlert A&B Test (SA Scientific, Inc.)

Binax NOW® Influenza A&B Test (Alere Scarborough, Inc.)

SAS™ Influenza B Test (SA Scientific, Inc.)

BioSign® Flu A+B or OraSure QuickFlu Rapid A+B Test or Polymedco Poly stat
Flu A&B Test or LifeSign LLC Status Flu A&B (Princeton BioMedtech Corp.)

Sofia® Analyzer and Influenza A+B FIA (CLIA-waived) (Quidel Corp.)

ClearView Exact II Influenza A&B Test or Alere Influenza A&B Test (Alere
Scarborough, Inc.)

TRU FLU® (Meridian Bioscience, Inc.)

OSOM® Influenza A&B Test (Sekisui Diagnostics)
QuickVue® Influenza A/B Test (Quidel Corp.)

SAS™ Influenza A Test (SA Scientific, Inc.)

Sofia® Analyzer and Influenza A+B FIA (Quidel Corp.)
XPECT™ Influenza A/B (Remel Inc./Thermo Fisher Scientific)
Other, specify:

Public reporting burden of this collection of information is estimated to average 10 minutes per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the
collection of information. An agency may not conduct or sponsor, and a person is not required to respond to a collection of information unless it displays a currently valid OMB Control Number. Send comments regarding this burden estimate or any other aspect of this
collection of information, including suggestions for reducing this burden to CDC/ATSDR Information Collection Request Office, 1600 Clifton Road NE, MS D-74, Atlanta, Georgia 30333; ATTN: PRA (0920-0978).

4c. What does the laboratory do if a rapid influenza antigen diagnostic test result is negative for influenza? (Select one) (Consider tests performed in
laboratory or other locations in the hospital, e.g. Emergency Department)
Report the negative result and do nothing else

Report the negative result with a disclaimer asking the physician to submit
a second specimen for testing with a more sensitive assay

Reflex to molecular assay (PCR) for confirmation
Report the negative result and submit specimen to state/regional public
health lab for PCR confirmation

Other, specify:

4d. What does the laboratory do if a rapid influenza antigen diagnostic test result is positive for influenza? (Select one) (Consider tests performed in
laboratory or other locations in the hospital, e.g. Emergency Department)
Report the positive result and submit specimen to state/regional public
health lab for PCR confirmation

Report the positive result and do nothing else
Reflex to another influenza test for confirmation

Other, specify:

Reflex to a confirmatory test only if early in influenza season
or off-season
Report the positive result with a disclaimer asking the physician to submit a
second specimen for testing with a more sensitive assay

5. Does the laboratory perform rapid molecular assays (e.g. Alere-i™, cobas® Liat; results available ≤20 minutes) for influenza?
Yes  Answer questions 5a-5b

No  Skip to question 6

5a. What does the laboratory do if the rapid molecular assay is negative for influenza? (Select one)
Report the negative result and do nothing else

Report the negative result and submit specimen to state/regional public
health lab for PCR confirmation

Reflex to standard molecular assay (PCR) for confirmation
Report the negative result with a disclaimer asking the physician to submit
a second specimen for testing with a more sensitive assay

Other, specify:

5b. What does the laboratory do if the rapid molecular assay is positive for influenza? (Check all that apply)
Report the positive result and do nothing else

Report the positive result and submit specimen to state/regional public
health lab for PCR confirmation

Reflex to standard molecular assay (PCR) for confirmation
Report the positive result with a disclaimer asking the physician to submit a
second specimen for testing with a standard molecular assay

Reflex for subtyping
Other, specify:

6. Does the laboratory perform standard molecular assays (e.g., RT-PCR; with results available > 20 minutes) for influenza?
Yes  Answer questions 6a-6c

No  Answer question 7

6a. Select kit name(s) (manufacturer) for all molecular assays performed at the laboratory: (Check all that apply)
(http://www.cdc.gov/flu/professionals/diagnosis/molecular-assays.htm)
Alere i NAT Flu A/B (CLIA Waived), (Alere)

Prodesse PROFLU™, (GenProbe/Hologic)

Alere i NAT Flu A/B (Moderate), (Alere)

Prodesse ProFAST™, (GenProbe/Hologic)

ARIES® Flu A/B & RSV Assay, (Luminex)

Qiagen Artus Influenza A/B Rotor-gene RT-PCR kit, (Qiagen)

CDC Human Influenza Virus Real-Time RT-PCR Diagnostic Panel (Influenza
A/B Typing Kit4), (CDC Influenza Division)

Quidel Molecular Influenza A+B, (Quidel)

CDC Human Influenza Virus Real-Time RT-PCR Detection and
Characterization Panel, (CDC Influenza Division)

Simplexa™ Flu A/B & RSV Direct, (Focus Diagnostics, 3M)

CDC Influenza A/H5 (Asian Lineage) Virus Real-Time RT-PCR Primer and
Probe Set, (CDC Influenza Division)

U.S. Army JBAIDS Influenza A&B Detection Kit , (Biofire Defense)

Simplexa™ Flu A/B & RSV, (Focus Diagnostics, 3M)
Simplexa™ Influenza A H1N1 (2009), (Focus Diagnostics, 3M)

CDC Influenza 2009 A(H1N1)pdm Real-Time RT-PCR Panel,
(CDC Influenza Division)

U.S. Army JBAIDS Influenza A Subtyping Kit, (Biofire Defense)

Cepheid Xpert Flu Assay, (Cepheid)

Verigene® Respiratory Virus Nucleic Acid Test, (Nanosphere, Inc)

Cepheid Xpert Flu/RSV XC Assay, (Cepheid)

Verigene® Respiratory Virus Plus Nucleic Acid Test (RV+),
(Nanosphere, Inc)

U.S. Army JBAIDS Influenza A/H5 Kit ,(Biofire Defense)

Cepheid Xpert Express Flu Assay, (Cepheid)

Verigene® Respiratory Pathogen Nucleic Acid Test (RP Flex),
(Nanosphere, Inc)

Cepheid Xpert Express Flu/RSV Assay, (Cepheid)
Cobas Liat Influenza A/B, (Roche Diagnostics)

x-TAG® Respiratory Viral Panel (RVP),
(Luminex Molecular Diagnostics Inc)

Cobas Liat Influenza A/B & RSV, (Roche Diagnostics)
eSensor® Respiratory Viral Panel (RVP), (GenMark Diagnostics)
FilmArray Respiratory Panel, (BioFire Diagnostics, LLC)

x-TAG® Respiratory Viral Panel Fast (RVP FAST),
(Luminex Molecular Diagnostics Inc)

Ibis PLEX-ID Flu, (Ibis/Abbott)

In-house developed PCR assay

IMDx Flu A/B and RSV for Abbott m2000, (IMDx)

Other, specify:

Revised 12/2017

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6b. If more than one kit is selected above, please select the one kit that is (or will be) used most frequently for molecular assay at the laboratory during
the current influenza season:
Alere i NAT Flu A/B (CLIA Waived), (Alere)

Prodesse PROFLU™, (GenProbe/Hologic)

Alere i NAT Flu A/B (Moderate), (Alere)

Prodesse ProFAST™, (GenProbe/Hologic)

ARIES® Flu A/B & RSV Assay, (Luminex)

Qiagen Artus Influenza A/B Rotor-gene RT-PCR kit, (Qiagen)

CDC Human Influenza Virus Real-Time RT-PCR Diagnostic Panel (Influenza
A/B Typing Kit4), (CDC Influenza Division)

Quidel Molecular Influenza A+B, (Quidel)

CDC Human Influenza Virus Real-Time RT-PCR Detection and
Characterization Panel, (CDC Influenza Division)

Simplexa™ Flu A/B & RSV Direct, (Focus Diagnostics, 3M)

CDC Influenza A/H5 (Asian Lineage) Virus Real-Time RT-PCR Primer and
Probe Set, (CDC Influenza Division)

U.S. Army JBAIDS Influenza A&B Detection Kit , (Biofire Defense)

Simplexa™ Flu A/B & RSV, (Focus Diagnostics, 3M)
Simplexa™ Influenza A H1N1 (2009), (Focus Diagnostics, 3M)

CDC Influenza 2009 A(H1N1)pdm Real-Time RT-PCR Panel,
(CDC Influenza Division)

U.S. Army JBAIDS Influenza A Subtyping Kit, (Biofire Defense)

Cepheid Xpert Flu Assay, (Cepheid)

Verigene® Respiratory Virus Nucleic Acid Test, (Nanosphere, Inc)

Cepheid Xpert Flu/RSV XC Assay, (Cepheid)

Verigene® Respiratory Virus Plus Nucleic Acid Test (RV+),
(Nanosphere, Inc)

U.S. Army JBAIDS Influenza A/H5 Kit ,(Biofire Defense)

Cepheid Xpert Express Flu Assay, (Cepheid)

Verigene® Respiratory Pathogen Nucleic Acid Test (RP Flex),
(Nanosphere, Inc)

Cepheid Xpert Express Flu/RSV Assay, (Cepheid)
Cobas Liat Influenza A/B, (Roche Diagnostics)

x-TAG® Respiratory Viral Panel (RVP),
(Luminex Molecular Diagnostics Inc)

Cobas Liat Influenza A/B & RSV, (Roche Diagnostics)
eSensor® Respiratory Viral Panel (RVP), (GenMark Diagnostics)
FilmArray Respiratory Panel, (BioFire Diagnostics, LLC)

x-TAG® Respiratory Viral Panel Fast (RVP FAST),
(Luminex Molecular Diagnostics Inc)

Ibis PLEX-ID Flu, (Ibis/Abbott)

In-house developed PCR assay

IMDx Flu A/B and RSV for Abbott m2000, (IMDx)

Other, specify:

6c. Does the laboratory perform influenza A virus subtyping?
Yes  Answer question 6d

No  Skip to question 7

6d. What testing kit does the testing facility use (or will it use) most often to perform influenza A sub-typing during the current influenza season?
(Select one)
Alere i NAT Flu A/B (CLIA Waived), (Alere)

Prodesse PROFLU™, (GenProbe/Hologic)

Alere i NAT Flu A/B (Moderate), (Alere)

Prodesse ProFAST™, (GenProbe/Hologic)

ARIES® Flu A/B & RSV Assay, (Luminex)

Qiagen Artus Influenza A/B Rotor-gene RT-PCR kit, (Qiagen)

CDC Human Influenza Virus Real-Time RT-PCR Diagnostic Panel (Influenza
A/B Typing Kit4), (CDC Influenza Division)

Quidel Molecular Influenza A+B, (Quidel)

CDC Human Influenza Virus Real-Time RT-PCR Detection and
Characterization Panel, (CDC Influenza Division)

Simplexa™ Flu A/B & RSV Direct, (Focus Diagnostics, 3M)

CDC Influenza A/H5 (Asian Lineage) Virus Real-Time RT-PCR Primer
and Probe Set, (CDC Influenza Division)

U.S. Army JBAIDS Influenza A&B Detection Kit , (Biofire Defense)

Simplexa™ Flu A/B & RSV, (Focus Diagnostics, 3M)
Simplexa™ Influenza A H1N1 (2009), (Focus Diagnostics, 3M)

CDC Influenza 2009 A(H1N1)pdm Real-Time RT-PCR Panel,
(CDC Influenza Division)

U.S. Army JBAIDS Influenza A Subtyping Kit, (Biofire Defense)

Cepheid Xpert Flu Assay, (Cepheid)

Verigene® Respiratory Virus Nucleic Acid Test, (Nanosphere, Inc)

Cepheid Xpert Flu/RSV XC Assay, (Cepheid)

Verigene® Respiratory Virus Plus Nucleic Acid Test (RV+),
(Nanosphere, Inc)

U.S. Army JBAIDS Influenza A/H5 Kit ,(Biofire Defense)

Cepheid Xpert Express Flu Assay, (Cepheid)

Verigene® Respiratory Pathogen Nucleic Acid Test (RP Flex),
(Nanosphere, Inc)

Cepheid Xpert Express Flu/RSV Assay, (Cepheid)
Cobas Liat Influenza A/B, (Roche Diagnostics)

x-TAG® Respiratory Viral Panel (RVP),
(Luminex Molecular Diagnostics Inc)

Cobas Liat Influenza A/B & RSV, (Roche Diagnostics)
eSensor® Respiratory Viral Panel (RVP), (GenMark Diagnostics)
FilmArray Respiratory Panel, (BioFire Diagnostics, LLC)

x-TAG® Respiratory Viral Panel Fast (RVP FAST),
(Luminex Molecular Diagnostics Inc)

Ibis PLEX-ID Flu, (Ibis/Abbott)

In-house developed PCR assay

IMDx Flu A/B and RSV for Abbott m2000, (IMDx)

Other, specify:

7. Does the laboratory perform any of the following additional tests to detect influenza (other than PCR or RIDT)? (Check all that apply)
Viral culture

Serology (IgG or IgM)

Indirect fluorescent antibody (IFA) stain

No

Direct fluorescent antibody (DFA) stain
8a. Which influenza test method does the laboratory perform most frequently for pediatric patients (aged 0-17 years)? (Select one)
Viral culture

Standard Molecular assay (e.g. RT-PCR, NAAT) – singleplex (influenza only)

Indirect fluorescent antibody (IFA)/direct fluorescent antibody stain (DFA)

Standard Molecular assay (e.g. RT-PCR, NAAT) – dualplex (influenza/RSV)

Rapid influenza antigen diagnostic test (rapid test, RIDT)

Standard Molecular assay (e.g. RT-PCR, NAAT) – multiplex/respiratory
viral panel (RVP)

Rapid Molecular assay (e.g. RT-PCR, NAAT) – singleplex (influenza only)
Rapid Molecular assay (e.g. RT-PCR, NAAT) – dualplex (influenza/RSV)

FluSurv-NET Laboratory Survey 2017–2018 Season

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8b. Which influenza test method does the laboratory perform most frequently for adult patients (aged ≥18 years)? (Select one)
Viral culture

Standard Molecular assay (e.g. RT-PCR, NAAT) – singleplex (influenza only)

Indirect fluorescent antibody (IFA)/direct fluorescent antibody stain (DFA)

Standard Molecular assay (e.g. RT-PCR, NAAT) – dualplex (influenza/RSV)

Rapid influenza antigen diagnostic test (rapid test, RIDT)

Standard Molecular assay (e.g. RT-PCR, NAAT) – multiplex/respiratory viral
panel (RVP)

Rapid Molecular assay (e.g. RT-PCR, NAAT) – singleplex (influenza only)
Rapid Molecular assay (e.g. RT-PCR, NAAT) – dualplex (influenza/RSV)

9. Based on tests that were performed during the 2016-2017 influenza season, approximately what percent of the time are each of these test types used
to test for flu overall? (Answers should add to 100%)
% Viral culture
% Indirect fluorescent antibody stain (IFA)/direct fluorescent antibody stain (DFA)
% Rapid influenza antigen diagnostic test (rapid test, RIDT)
% Rapid Molecular assay (e.g. RT-PCR, NAAT) – singleplex (influenza only)
% Rapid Molecular assay (e.g. RT-PCR, NAAT) – dualplex (influenza/RSV)
% Standard Molecular assay (e.g. RT-PCR, NAAT) – singleplex (influenza only)
% Standard Molecular assay (e.g. RT-PCR, NAAT) – dualplex (influenza/RSV)
% Standard Molecular assay (e.g. RT-PCR, NAAT) – multiplex/respiratory viral panel (RVP)
10. Does the lab send specimens to other labs for clinical testing of influenza (select all that apply)?
Yes, Commercial lab(s): List names of all labs:

Yes, Public Health lab(s): List names of all labs:

Yes, Other lab(s): List names of all labs:

No
11. Laboratory comments:

RSV
Question
12. Does the laboratory perform diagnostic testing for RSV on-site?
No  Answer question 12a

Yes  Answer question 13

12a. What are the reasons that the laboratory does not perform testing for RSV? (Check all that apply) (Then skip to Question 21)
Cost prohibitive

Inadequate staffing (not enough staff or lack of staff training)

Results not available in a timely manner

Other (specify):

13. Does the laboratory perform rapid antigen detection tests (RADT) for RSV?
Yes, pediatric patients only  Answer questions 13a-13b

Yes, pediatric and adult patients  Answer questions 13a-13b

Yes, adult patients only  Answer questions 13a-13b

No  Skip to question 14

13a. Select the kit name(s) (manufacturer) for the RSV rapid antigen detection test(s) performed at the laboratory: (Check all that apply)
BinaxNOW® RSV Card (Alere Scarborough, Inc.)

RAMP™ Rapid Detection RSV Test Kit (Response Biomedical Corp.)

Clearview® RSV (Alere Scarborough, Inc.)

SAS™ RSVAlert (SA Scientific, Inc.)

QuickVue RSV Test (Quidel Corp.)

Xpect™ RSV Test (Remel Inc./Thermo Fisher Scientific)

Sofia RSV FIA (Quidel Corp.)

BD Veritor System for Rapid Detection of RSV (Becton-Dickinson & Co.)

Directigen™ EZ RSV Kit (Becton-Dickinson & Co.)

Other, specify:

TRU RSV® Kit (Meridian Bioscience, Inc.)
13b. If more than one kit is selected above, please select the one kit that is (or will be) used most frequently for RSV rapid antigen detection testing at the
laboratory during the current RSV season: (Select one)
BinaxNOW® RSV Card (Alere Scarborough, Inc.)

RAMP™ Rapid Detection RSV Test Kit (Response Biomedical Corp.)

Clearview® RSV (Alere Scarborough, Inc.)

SAS™ RSVAlert (SA Scientific, Inc.)

QuickVue RSV Test (Quidel Corp.)

Xpect™ RSV Test (Remel Inc./Thermo F+isher Scientific)

Sofia RSV FIA (Quidel Corp.)

BD Veritor System for Rapid Detection of RSV (Becton-Dickinson & Co.)

Directigen™ EZ RSV Kit (Becton-Dickinson & Co.)

Other, specify:

TRU RSV® Kit (Meridian Bioscience, Inc.)
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14. Does the laboratory perform molecular assays (e.g., RT-PCR) for RSV?
Yes, pediatric patients only  Answer questions 14a-14b

Yes, pediatric and adult patients  Answer questions 14a-14b

Yes, adult patients only  Answer questions 14a-14b

No  Skip to question 15

14a. Select kit name(s) (manufacturer) for all molecular assays used at the laboratory: (Check all that apply)
ARIES® Flu A/B & RSV Assay (Luminex)

Verigene® Respiratory Virus Plus Nucleic Acid Test (RV+) (Nanosphere, Inc)

Alere™ i RSV (Alere)
Cepheid Xpert Flu/RSV XC Assay (Cepheid)

Verigene® Respiratory Pathogen Nucleic Acid Test (RP Flex)
(Nanosphere, Inc)

eSensor® Respiratory Viral Panel (RVP) (GenMark Diagnostics)

x-TAG® Respiratory Viral Panel (RVP) (Luminex Molecular Diagnostics Inc)

FilmArray Respiratory Panel (BioFire Diagnostics LLC)

x-TAG® Respiratory Viral Panel Fast (RVP FAST) (Luminex Molecular
Diagnostics Inc)

IMDx Flu A/B and RSV for Abbott m2000 (IMDx)

In-house developed PCR assay

Prodesse PROFLU™+ (GenProbe/Hologic)

CDC Respiratory Syncytial Virus Real-Time RT-PCR Assay

Simplexa™ Flu A/B & RSV (Focus Diagnostics, 3M)

Other, specify:

Simplexa™ Flu A/B & RSV Direct (Focus Diagnostics, 3M)
Verigene® Respiratory Virus Nucleic Acid Test (Nanosphere, Inc)

14b. If more than one kit is selected above, please select the one kit that is (or will be) used most frequently for molecular assays at the laboratory during
the current RSV season: (Select one)
ARIES® Flu A/B & RSV Assay (Luminex)

Verigene® Respiratory Virus Plus Nucleic Acid Test (RV+) (Nanosphere, Inc)

Alere™ i RSV (Alere)
Cepheid Xpert Flu/RSV XC Assay (Cepheid)

Verigene® Respiratory Pathogen Nucleic Acid Test (RP Flex)
(Nanosphere, Inc)

eSensor® Respiratory Viral Panel (RVP) (GenMark Diagnostics)

x-TAG® Respiratory Viral Panel (RVP) (Luminex Molecular Diagnostics Inc)

FilmArray Respiratory Panel (BioFire Diagnostics, LLC)

x-TAG® Respiratory Viral Panel Fast (RVP FAST) (Luminex Molecular
Diagnostics Inc)

IMDx Flu A/B and RSV for Abbott m2000 (IMDx)

In-house developed PCR assay

Prodesse PROFLU™+ (GenProbe/Hologic)

CDC Respiratory Syncytial Virus Real-Time RT-PCR Assay

Simplexa™ Flu A/B & RSV (Focus Diagnostics, 3M)

Other, specify:

Simplexa™ Flu A/B & RSV Direct (Focus Diagnostics, 3M)
Verigene® Respiratory Virus Nucleic Acid Test (Nanosphere, Inc)

15. Does the laboratory perform any of these additional tests to detect RSV (apart from rapid antigen detection tests and molecular assays) for pediatric
patients (aged 0–17 years)? (Select all that apply)
Viral culture

Serology (IgG or IgM)

Indirect fluorescent antibody (IFA) stain

None of the above

Direct fluorescent antibody (DFA) stain

Not applicable (only do testing in adult patients)

16. Does the laboratory perform any of these additional tests to detect RSV (apart from rapid antigen detection tests and molecular assays) for adult
patients (aged ≥ 18 years)? (Select all that apply)
Viral culture

Serology (IgG or IgM)

Indirect fluorescent antibody (IFA) stain

None of the above

Direct fluorescent antibody (DFA) stain

Not applicable (only do testing in pediatric patients)

17. Which RSV test method does the laboratory perform most frequently for pediatric patients (aged 0–17 years)? (Select one)
Viral culture

Molecular assay (e.g. RT-PCR, NAAT) – singleplex (RSV only)

Indirect fluorescent antibody stain (IFA)/direct fluorescent antibody stain (DFA)

Molecular assay (e.g. RT-PCR, NAAT) – dualplex (RSV/influenza)

Serology (IgG or IgM)

Molecular assay (e.g. RT-PCR, NAAT) – multiplex/respiratory viral panel (RVP)

Rapid antigen detection test (rapid test, RADT)

Not applicable (only do testing in adult patients)

18. Which RSV test method does the laboratory perform most frequently for adult patients (aged ≥ 18 years)? (Select one)
Viral culture

Molecular assay (e.g. RT-PCR, NAAT) – singleplex (RSV only)

Indirect fluorescent antibody stain (IFA)/direct fluorescent antibody stain (DFA)

Molecular assay (e.g. RT-PCR, NAAT) – dualplex (RSV/influenza)

Serology (IgG or IgM)

Molecular assay (e.g. RT-PCR, NAAT) – multiplex/respiratory viral panel (RVP)

Rapid antigen detection test (rapid test, RADT)

Not applicable (only do testing in pediatric patients)

19. Based on tests that were performed during the 2016-2017 RSV season, approximately what percent of the time are each of these test types used to
test for RSV in pediatric patients (aged 0–17) years? (Answers should add to 100%)
% Viral culture
% Indirect fluorescent antibody stain (IFA)/direct fluorescent antibody stain (DFA)
% Serology (IgG or IgM)
% Rapid antigen detection test (rapid test, RADT)
% Molecular assay (e.g. RT-PCR, NAAT) – singleplex (RSV only)
% Molecular assay (e.g. RT-PCR, NAAT) – dualplex (RSV/influenza)
% Molecular assay (e.g. RT-PCR, NAAT) – multiplex/respiratory viral panel (RVP)
Not applicable (only do testing in adults patients)
FluSurv-NET Laboratory Survey 2017–2018 Season

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20. Based on tests that were performed during the 2016-2017 RSV season, approximately what percent of the time are each of these test types used to
test for RSV in adult patients (aged ≥ 18 years)? (Answers should add to 100%)
% Viral culture
% Indirect fluorescent antibody stain (IFA)/direct fluorescent antibody stain (DFA)
% Serology (IgG or IgM)
% Rapid antigen detection test (rapid test, RADT)
% Molecular assay (e.g. RT-PCR, NAAT) – singleplex (RSV only)
% Molecular assay (e.g. RT-PCR, NAAT) – dualplex (RSV/influenza)
% Molecular assay (e.g. RT-PCR, NAAT) – multiplex/respiratory viral panel (RVP)
Not applicable (only do testing in pediatric patients)
21. Does the lab send specimens to other labs for clinical testing of RSV (select all that apply)?
Yes, Commercial lab(s): List names of all labs:

Yes, Public Health lab(s): List names of all labs:

Yes, Other lab(s): List names of all labs:

No
22. Laboratory comments:

FluSurv-NET site use only
23. List all FluSurv-NET hospital IDs (hosp_TX) associated with this testing facility.
Hospital ID 1 (Required):

Hospital ID 6:

Hospital ID 2:

Hospital ID 7:

Hospital ID 3:

Hospital ID 8:

Hospital ID 4:

Hospital ID 9:

Hospital ID 5:

Hospital ID 10:

End of survey. Thank you for participating in this survey!

Revised 12/2017

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