Form 0920-21BA US NAC Webinar Polling Questions

^{NAC Webinar Survey}/Polling Questions

1. My facility changed operations due to COVID-19 as follows: (select all that apply)

• Laboratory stopped work

• Limited staff onsite

• Changes to cleaning/decontamination practices

• Changes to PPE

• Other

• None

2. My facility has: (select all that apply)

• PV infectious materials

• PV potentially infectious materials (PIM) [e.g., fecal, respiratory, environmental specimens]

• Nucleic acids extracted from the materials listed above

• nOPV2

• S19

• No PV relevant materials right now but future work is planned

• Unsure
  
  

3. Has your facility submitted any of the following types of surveys? (Select all that apply)

• Institution-level (single survey for all labs, facility wide)

• Campus-level (individual surveys for each/all of the facility’s sites)

• Campus-level (individual surveys for only some of the facility’s sites)

• Department level (all labs within my department)

• Laboratory-level (each lab submitted their own survey)

• Unsure

4. How likely is it that your facility can coordinate a full institution-level survey? (Select one)

• Very likely - Not challenging at all

• Somewhat likely - It would take some time, but we could do it

• Unlikely - Too difficult to achieve/We don’t have the resources

• Unsure

5. How do you use your collection of PIM samples? (check all that apply)

• Retrospective studies

• Discovery of new viruses

• Assay development

• Reference collection

• Environmental surveillance

• Storage only

• Other

• We do not have PIM

6. The WHO risk level for our work with PIM is: (if have multiple PIM types/procedures, select highest level)

• High (WPV/VDPV PIM)

• Moderate (e.g., OPV/Sabin PIM - fecal sample inoculation in PV-permissive cells)

• Low (e.g., OPV/Sabin PIM - respiratory sample inoculation in PV-permissive cells)

• Lowest (e.g., OPV/Sabin PIM - extracted nucleic acids used in PCR)

• Storage only

• Unsure

• We do not have PIM

7. My facility considers the risks associated with our PIM work as: (if have multiple PIM types/procedures, select highest level)

• High

• Moderate

• Low

• Lowest

• Storage only

• Unsure

• We do not have PIM

8. I feel the WHO risk level classification for PIM is appropriate.

• Strongly agree

• Agree

• Neither agree or disagree

• Disagree

• Strongly disagree

9. The appropriate laboratory containment practices for wild or vaccine derived PIM should be: (select best answer)

• GAPIII containment

• Same as WHO OPV/Sabin PIM guidance

• BSL-3 standards (BMBL)

• BSL-2 standards (BMBL)

• Combination of practices

• Unsure

10. My PIM collection has important scientific value and serves a critical national or international function.

• Strongly agree

• Agree

• Neither agree or disagree

• Disagree

• Strongly disagree

• We do not have PIM

11. Destroying or extracting nucleic acids from PIM will not permit our facility to perform critical studies including:

Text box – 200 words maximum

12. The recommended modifications to nucleic acid extraction methods: (select all that apply)

• Will allow us to inactivate our entire PIM collection

• Will allow us to inactivate some PIM samples

• Require internal validation for our testing protocols

• Require coordination with collaborators if we make these changes

• Not applicable to our work

• Unsure

13. Which of the following proposed policies is the highest priority for your facility?

• Incident response

• Occupational health (immunizations)

• PIM Guidance for U.S. facilities

• Unsure

14. How do you share PV materials with collaborators? (select all that apply)

• Intra-facility with other labs

• Domestic collaborators

• International collaborators

• We do not share our collection

15. Once containment is required, my facility plans to:

• Complete work

• Continue work (seek ICC/CC)

• Undecided

16. My facility has the following enhanced physical lab features for PV materials: (select all that apply)

• Anteroom/Airlock

• Inward directional airflow

• Exit shower

• Pass-through autoclave

• Effluent decontamination system

• Sealable for gaseous decontamination

• None of the above

17. Occupational health requirements for working with poliovirus materials at my facility include: (select all that apply)

• Verification of childhood PV immunization records

• Adult IPV booster

• IPV booster every 3 years

• Baseline PV serum neutralizing antibody titers ≥ 1:8

• Annual PV serum neutralizing antibody titers ≥ 1:8

• None of the above

• Unsure

18. Security controls for poliovirus materials at my facility include: (select all that apply)

• Locked freezers

• Limited access to laboratory or storage areas

• Biosecurity training

• Personnel reliability program

• None of the above

• Unsure

19. What does 10% bleach mean in free chlorine parts per million (ppm)?

• 52,500 ppm

• 25,000 ppm

• 10,000 ppm

• 5,250 ppm

• 2,500 ppm

• Unsure

20. NAC recommends using at least _______ free chlorine parts per million (ppm) for PV decontamination.

• 52,500 ppm

• 25,000 ppm

• 10,000 ppm

• 5,250 ppm

• 2,500 ppm

• Unsure

21. My facility conducts studies with PV materials in: (select all that apply)

• PVR transgenic mice

• Non-human primates

• Other animal model

• We do not use animals with PV materials

22. Standard PPE practices used in my facility for PV materials include: (select all that apply)

• Safety glasses

• Face shield

• Double gloves

• Face or surgical mask

• Respirator/N95

• Flat front gown

• Coverall

• Shoe covers/dedicated shoes

• Clothing change

• Showering out

23. For PPE selected above, identify the manufacturer and product/catalog number.

Text box – 100 words maximum

24. I prefer alternative options rather than a mandatory exit shower (e.g., more PPE, add decon steps during doffing).

• Strongly agree

• Agree

• Neither agree or disagree

• Disagree

• Strongly disagree

25. For PV materials, my facility uses surgical/isolation gowns or coveralls meeting standards for:

• Fluid resistance (e.g., level 1, 2, or 3)

• Viral penetration resistance (e.g., level 4)

• Testing standards are not currently considered for PPE

• Unsure

• Not applicable – gowns/coveralls are not worn

26. For PV materials, my facility uses tape to seal/secure gloves to sleeves of PPE.

• Yes

• No

27. For PV materials, my facility disinfects disposable PPE (e.g., gowns, coveralls) prior to reuse with:

• Chlorine (e.g., bleach)

• Alcohol (e.g., ethanol)

• Oxidizing agents (e.g., Oxivir, Virkon-S)

• Quaternary ammonium (e.g.,Roccal, Clorox or Lysol wipes)

• Other

• Unsure

• We do not reuse disposable PPE