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eLearning

BIOTHREAT PREPAREDNESS TRAINING FOR
SENTINEL LABORATORIES - Brucella spp.
Course Number
P.A.C.E.® Course Number: 288-012-17
FL Course Number: 20-515740

Description

This module has been developed to provide clinical laboratory scientists with information about the laboratory
identification of Brucella species.
The goal is to assist clinical laboratory scientists in better recognizing potential biothreat agents they might
encounter during routine laboratory work-ups of sputum, blood and aspirate/biopsy specimens.
Laboratory managers may consider using this course:
To assess staff competency in performing laboratory procedures accurately, and
To determine if staff are referring specimens for test confirmations correctly.

Objectives
At the conclusion of this program, the participant will be able to:
 Describe laboratory tests for identification of Brucella spp.
 Describe how to rule out or refer isolates for Brucella spp. using standardized laboratory protocols.
 Discuss the role of the sentinel laboratory in bioterrorism response.

Registration - Free Registration
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Locate the course online at www.cdc.gov/labtraining
Follow the link to register for the course in TRAIN
If you have difficulty with the online registration process, please email [email protected]
For additional program information, email [email protected] or call (404) 498-6022

Continuing Education
The Centers for Disease Control and Prevention Laboratory Training Team is approved as a provider of
continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E.® Program. This
course is approved for 1.5 contact hour.
This course has been approved for 1.5 contact hour in the category Microbiology/Mycology/Parasitology,
for Florida Laboratory Licensees.

Special Needs
Course content is closed captioned where applicable and optimized for a screen reader.

For a complete list of courses, visit www.cdc.gov/labtraining
Division of Laboratory Systems
Laboratory Training Team

eLearning

BIOTHREAT PREPAREDNESS TRAINING FOR
SENTINEL LABORATORIES - Burkholderia spp.
Course Number
P.A.C.E.® Course Number: 288-013-17
FL Course Number: 20-515742

Description

This module has been developed to provide clinical laboratory scientists with information about the laboratory
identification of Burkholderia spp.
The goal is to assist clinical laboratory scientists in better recognizing potential biothreat agents they might
encounter during routine laboratory work-ups of sputum, blood and aspirate/biopsy specimens.
Laboratory managers may consider using this course:
To assess staff competency in performing laboratory procedures accurately, and
To determine if staff are referring specimens for test confirmations correctly.

Objectives
At the conclusion of this program, the participant will be able to:
 Summarize the basic epidemiology and ecology of Burkholderia spp.
 List important aspects of biosafety in working with Burkholderia spp. in the laboratory.
 Outline the role of the LRN sentinel laboratory in the detection of Burkholderia spp.
 Apply various laboratory tests that are used to presumptively identify Burkholderia spp.
 Use sentinel laboratory protocols to Rule Out or Refer isolates for the identification of Burkholderia
spp.

Registration - Free Registration
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Locate the course online at www.cdc.gov/labtraining
Follow the link to register for the course in TRAIN
If you have difficulty with the online registration process, please email [email protected]
For additional program information, email [email protected] or call (404) 498-6022

Continuing Education
The Centers for Disease Control and Prevention Laboratory Training Team is approved as a provider of
continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E.® Program. This
course is approved for 1.0 contact hour.
This course has been approved for 1.0 contact hour in the category Microbiology/Mycology/Parasitology,
for Florida Laboratory Licensees.

Special Needs
Course content is closed captioned where applicable and optimized for a screen reader.

For a complete list of courses, visit www.cdc.gov/labtraining
Division of Laboratory Systems
Laboratory Training Team

eLearning

BIOTHREAT PREPAREDNESS TRAINING FOR
SENTINEL LABORATORIES - Bacillus anthracis
Course Number

P.A.C.E.® Course Number: 288-014-17
FL Course Number: 20-515744

Description

This module has been developed to provide clinical laboratory scientists with information about the laboratory
identification of Bacillus anthracis.
The goal is to assist clinical laboratory scientists in better recognizing potential biothreat agents they might
encounter during routine laboratory work-ups of sputum, blood and aspirate/biopsy specimens.
Laboratory managers may consider using this course:
To assess staff competency in performing laboratory procedures accurately, and
To determine if staff are referring specimens for test confirmations correctly.

Objectives
At the conclusion of this program, the participant will be able to:
 Describe laboratory tests for presumptive identification of Bacillus anthracis.
 Describe how to rule out or refer presumptive organisms for B. anthracis.
 Discuss the role of the LRN sentinel laboratory in detection of B. anthracis.

Registration - Free Registration
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Locate the course online at www.cdc.gov/labtraining
Follow the link to register for the course in TRAIN
If you have difficulty with the online registration process, please email [email protected]
For additional program information, email [email protected] or call (404) 498-6022

Continuing Education
The Centers for Disease Control and Prevention Laboratory Training Team is approved as a provider of
continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E.® Program. This
course is approved for 1.5 contact hour.
This course has been approved for 1.5 contact hour in the category Microbiology/Mycology/Parasitology,
for Florida Laboratory Licensees.

Special Needs
Course content is closed captioned where applicable and optimized for a screen reader.

For a complete list of courses, visit www.cdc.gov/labtraining
Division of Laboratory Systems
Laboratory Training Team

eLearning

BIOTHREAT PREPAREDNESS TRAINING FOR
SENTINEL LABORATORIES - Francisella tularensis
Course Number
P.A.C.E.® Course Number: 288-016-17
FL Course Number: 20-515746

Description

This module has been developed to provide clinical laboratory scientists with information about the laboratory
identification of Francisella tularensis.
The goal is to assist clinical laboratory scientists in better recognizing potential biothreat agents they might
encounter during routine laboratory work-ups of sputum, blood and aspirate/biopsy specimens.
Laboratory managers may consider using this course:
To assess staff competency in performing laboratory procedures accurately, and
To determine if staff are referring specimens for test confirmations correctly.

Objectives
At the conclusion of this program, the participant will be able to:
 Describe laboratory tests for presumptive identification of Francisella tularensis.
 Describe how to rule out or refer presumptive organisms for F. tularensis.
 Discuss the role of the sentinel laboratory in detection of F. tularensis.
 Describe the basic epidemiology and ecology of F. tularensis in the U.S. including animal reservoirs,
vectors, and transmission cycles.

Registration - Free Registration
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Locate the course online at www.cdc.gov/labtraining
Follow the link to register for the course in TRAIN
If you have difficulty with the online registration process, please email [email protected]
For additional program information, email [email protected] or call (404) 498-6022

Continuing Education
The Centers for Disease Control and Prevention Laboratory Training Team is approved as a provider of
continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E.® Program. This
course is approved for 1.5 contact hour.
This course has been approved for 1.5 contact hour in the category Microbiology/Mycology/Parasitology,
for Florida Laboratory Licensees.

Special Needs
Course content is closed captioned where applicable and optimized for a screen reader.

For a complete list of courses, visit www.cdc.gov/labtraining
Division of Laboratory Systems
Laboratory Training Team

eLearning

BIOTHREAT PREPAREDNESS TRAINING FOR
SENTINEL LABORATORIES - Yersinia pestis
Course Number
P.A.C.E.® Course Number: 288-015-17
FL Course Number: 20-515736

Description

This module has been developed to provide clinical laboratory scientists with information about the laboratory
identification of Yersinia pestis.
The goal is to assist clinical laboratory scientists in better recognizing potential biothreat agents they might
encounter during routine laboratory work-ups of sputum, blood and aspirate/biopsy specimens.
Laboratory managers may consider using this course:
To assess staff competency in performing laboratory procedures accurately, and
To determine if staff are referring specimens for test confirmations correctly.

Objectives
At the conclusion of this program, the participant will be able to:
 Summarize the basic epidemiology and ecology of Yersinia pestis.
 List important aspects of biosafety in working with Y. pestis in the laboratory.
 Outline the role of the LRN sentinel laboratory in the detection of Y. pestis.
 Apply various laboratory tests that are used to presumptively identify Y. pestis.
 Use sentinel laboratory protocols to Rule Out or Refer isolates for the identification of Y. pestis.

Registration - Free Registration
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Locate the course online at www.cdc.gov/labtraining
Follow the link to register for the course in TRAIN
If you have difficulty with the online registration process, please email [email protected]
For additional program information, email [email protected] or call (404) 498-6022

Continuing Education
The Centers for Disease Control and Prevention Laboratory Training Team is approved as a provider of
continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E.® Program. This
course is approved for 1.0 contact hour.
This course has been approved for 1.0 contact hour in the category Microbiology/Mycology/Parasitology,
for Florida Laboratory Licensees.

Special Needs
Course content is closed captioned where applicable and optimized for a screen reader.
For a complete list of courses, visit www.cdc.gov/labtraining
Division of Laboratory Systems
Laboratory Training Team

Basic Culture Media

Online Course

Basic Microbiology Curriculum

Sponsored by the Centers for Disease Control and Prevention Laboratory Training Team

| DESCRIPTION |

| REGISTRATION |

This eLearning course is designed to familiarize
laboratorians with basic culture media used in the
microbiology laboratory. Laboratorians will review and
contrast the various culture medias, describe the process
of streaking a plate, identify types of colonial morphology
and explain commonly encountered problems that occur
with culture media.

FREE REGISTRATION
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

Register online at www.cdc.gov/labtraining
If you have difficulty with the online registration
process, please email [email protected]

| CONTINUING EDUCATION |

| AUDIENCE |
New or existing public health laboratorians, who have a
science background, are entering or reentering the
microbiology field and who need training in routine basic
culture media essential for performing job requirements.

The Centers for Disease Control and Prevention
Laboratory Training Branch is approved as a provider of
continuing education programs in the clinical laboratory
sciences by the ASCLS P.A.C.E.® Program. This course is
approved for 1.0 contact hours.

| OBJECTIVES |

This course has been approved for 1.0 contact hours in
the category of Microbiology/Mycology/Parasitology for
Florida Laboratory Licensees.

At the conclusion of this program, the participant will be
able to:
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Distinguish enrichment, differential, and selective
media.
Review the principles of the most commonly used
media.
Contrast various culture media.
Describe the steps of streaking a plate.
Interpret results on various culture media.
Recognize the different types of colonial morphology.
Identify commonly encountered problems with basic
culture media.

| SPECIAL NEEDS |
Course content is closed captioned where applicable
and optimized for a screen reader.

P.A.C.E. Course Number: 288-008-18
FL Course Number: 20-435308

For a complete list of courses, visit www.cdc.gov/labtraining.

BASIC MICROSCOPY
BASIC MICROBIOLOGY CURRICULUM
PROVIDED BY: CDC LABORATORY TRAINING AND SERVICES

AN ONLINE LEARNING/ELEARNING COURSE
AVAILABLE AT WWW.CDC.TRAIN.ORG

DESCRIPTION

CONTINUING EDUCATION

To function effectively in a microbiology laboratory,
laboratorians must have a basic knowledge and
understanding of the components, setup, procedures, and
care and maintenance of a bright field (compound)
microscope. This eLearning course will instruct participants
on the importance of a correctly setup bright field
microscope for the identification of microorganisms.

The Centers for Disease Control and Prevention
Laboratory Training and Services Branch is approved as
a provider of continuing education programs in the
clinical laboratory sciences by the ASCLS P.A.C.E.®
Program. Each course module is approved for 1.0
contact hours.

AUDIENCE
This basic level course is designed for new or existing
public health laboratorians, who have a science
background, are entering or reentering the microbiology
field and who need training in basic microscopy principles
and techniques essential for performing job requirements.

This course has been approved for 1.0 contact hours in
the category of Microbiology/Mycology/Parasitology
for Florida Laboratory Licensees.

OBJECTIVES
At the conclusion of this program, the participant will
be able to:
• Identify the major components of the microscope and
their function.

SPECIAL NEEDS

• Identify how to maintain a microscope.

Course content is closed captioned where applicable and
optimized for a screen reader.

• Discuss the role of Kohler illumination in microscopy.

FREE REGISTRATION
·
·
·

Register online at www.cdc.gov/labtraining
If you have difficulty with the online registration
process, please email [email protected]
For additional program information, email
[email protected] or call (404) 498-6022

• Describe the process to correctly focus on the
appropriate field of view.
• Use the ocular micrometer to measure an object
under the microscope.
• Demonstrate the ability to troubleshoot encountered
problems with the microscope.

CE INFORMATION
P.A.C.E.™ Course Number: 288-019-17
FL Course Number: 20-434651

For a complete list of courses, visit www.cdc.gov/labtraining.

Routine Microscopy Procedures
Basic Microbiology Curriculum
SPONSORED BY THE CENTERS FOR DISEASE CONTROL AND
PREVENTION LABORATORY TRAINING BRANCH

AN ONLINE LEARNING/ELEARNING COURSE
AVAILABLE AT WWW.CDC.TRAIN.ORG

DESCRIPTION

CONTINUING EDUCATION

This eLearning course is designed to familiarize
laboratorians with routine microscopy procedures used in
the microbiology laboratory. Laboratorians will explore the
required steps for a smear preparation as well as the
requirements for preparing and interpreting the results of
Gram stains, wet mounts, potassium hydroxide (KOH), and
India Ink procedures.

The Centers for Disease Control and Prevention
Laboratory Training Branch is approved as a provider of
continuing education programs in the clinical laboratory
sciences by the ASCLS P.A.C.E.® Program. This course is
approved for 1.5 contact hours.

AUDIENCE
New or existing public health laboratorians, who have a
science background, are entering or reentering the
microbiology field and who need training in routine
microscopy techniques essential for performing job
requirements.

SPECIAL NEEDS
Course content is closed captioned where applicable and
optimized for a screen reader.

This course has been approved for 1.5 contact hours in
the category of Microbiology/Mycology/Parasitology
for Florida Laboratory Licensees.

OBJECTIVES
At the conclusion of this program, the participant will
be able to:
At the conclusion of this program, the participant will
be able to:
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FREE REGISTRATION
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·
·

Register online at www.cdc.gov/labtraining
If you have difficulty with the online registration
process, please email [email protected]
For additional program information, email
[email protected] or call (404) 498-6022

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CE INFORMATION
P.A.C.E.™ Course Number: 288-020-17
FL Course Number: 20-434648



Outline the steps of preparing a smear.
Express the purpose of the Gram stain
procedure.
Identify the types of reagents used in the Gram
stain procedure.
Sequence the steps in the Gram stain
procedure.
Interpret the results seen in the bacterial cells,
with the effects of the various reagents during
the Gram stain procedure.
Outline the potassium hydroxide (KOH)
procedure and its uses.
Identify how to prepare and interpret a wet
mount.
Identify the steps and results obtained in the
India ink procedure.
Identify and resolve commonly encountered
problems during routine microscopy
procedures.

For a complete list of courses, visit www.cdc.gov/labtraining.

Online Course

Biochemicals and Gram Positive Organism ID
Basic Microbiology Curriculum
Sponsored by the Centers for Disease Control and Prevention, Division of Laboratory
Systems, Laboratory Training and Services Branch

| DESCRIPTION |

| REGISTRATION |

This eLearning course is designed to familiarize
laboratorians with how to read a Gram stain, the
colonial characteristics and biochemical tests used to
identify Gram positive microorganisms as well as
commonly used testing algorithms. The course will only
cover aerobic microorganisms, those that grow in the
presence of oxygen.

FREE REGISTRATION
 Locate the course online at www.cdc.gov/labtraining
 Follow the link to register for the course in TRAIN
 If you have difficulty with the online registration process,

please email [email protected]
 For additional program information, email
[email protected] or call (404) 498-6022

| CONTINUING EDUCATION |

| AUDIENCE |
New or existing public health and clinical laboratorians
who have a science background, are entering or
reentering the microbiology field and who need training
in biochemical test methods and Gram positive
microorganism identification essential for performing
job requirements.

The Centers for Disease Control and Prevention Laboratory
Training Branch is approved as a provider of continuing
education programs in the clinical laboratory sciences by
the ASCLS P.A.C.E.® Program. This course is approved for
1.5 contact hours.
This course has been approved for 1.5 contact hours in the
category of Microbiology/Mycology/Parasitology for Florida
Laboratory Licensees.

| OBJECTIVES |
At the conclusion of this program, the participant will be
able to:
 Identify different types of bacterial morphology seen
on a Gram stain
 Identify different types of colonial characteristics
 Use flowcharts and identification charts to identify
some common aerobic Gram positive microorganisms
 Associate various biochemical tests with their correct
applications
 Interpret the results of biochemical methods

| SPECIAL NEEDS |
Course content is closed captioned where applicable and
optimized for a screen reader.

P.A.C.E.® Course Number: 288-020-18
FL Course Number: 20-541331

For a complete list of courses, visit www.cdc.gov/labtraining.

Online Course 

Biochemicals and Gram Negative Organism ID
Basic Microbiology Curriculum
Sponsored by Centers for Disease Control and Prevention Laboratory Training and Services 
Branch, Division of Laboratory Systems 

|  DESCRIPTION  | 

|  REGISTRATION  | 

This eLearning course is designed to familiarize 
laboratorians with how to read a Gram stain, colonial 
characteristics, and biochemical tests used to identify 
Gram negative microorganisms as well as commonly 
used testing algorithms. The course will only cover 
aerobic microorganisms, those that grow in the 
presence of oxygen. 

FREE REGISTRATION 
 Locate the course online at www.cdc.gov/labtraining
 Follow the link to register for the course in TRAIN
 If you have difficulty with the online registration process,

please email  [email protected]
 For additional program information, email
[email protected]  or call (404) 498‐6022 

|  CONTINUING EDUCATION  |

|  AUDIENCE  | 
New or existing public health and clinical laboratorians, 
who have a science background, are entering or 
reentering the microbiology field and who need training 
in biochemical test methods and Gram negative 
microorganism identification essential for performing 
job requirements.  

The Centers for Disease Control and Prevention Laboratory 
Training Branch is approved as a provider of continuing 
education programs in the clinical laboratory sciences by 
the ASCLS P.A.C.E.® Program. This course is approved for 
1.0 contact hours. 
This course has been approved for 1.0 contact hours in the 
category of Microbiology/Mycology/Parasitology for Florida 
Laboratory Licensees.  

|  OBJECTIVES  |
At the conclusion of this program, the participant will be 
able to: 
 Identify different types of bacterial morphology
seen on a Gram stain
 Identify different types of colonial characteristics
 Use flowcharts and identification charts to
identify some common aerobic Gram negative
microorganisms
 Associate various biochemical tests with their
correct applications
 Interpret the results of biochemical methods

|  SPECIAL NEEDS  |
Course content is closed captioned where applicable and 
optimized for a screen reader. 

P.A.C.E.® Course Number: 288‐021‐18 
FL Course Number:  20‐446817' 

For a complete list of courses, visit www.cdc.gov/labtraining. 

MASTER: Antimicrobial Susceptibility Testing
Methods
SPONSORED BY THE CENTERS FOR DISEASE CONTROL AND
PREVENTION OFFICE OF ANTIMICROBIAL RESISTANCE

AN ONLINE LEARNING/ELEARNING COURSE
AVAILABLE AT WWW.CDC.TRAIN.ORG

DESCRIPTION

CONTINUING EDUCATION

This course provides a detailed overview of antimicrobial
susceptibility testing methods including reference
methods such as those described by the Clinical and
Laboratory Standards Institute (CLSI) and commercial test
systems. It focuses on technical and regulatory
considerations for any method of medical laboratory
antimicrobial susceptibility testing. The course provides
essential information for those new to antimicrobial
susceptibility testing and an important refresher for
laboratory professionals with more experience.

The Centers for Disease Control and Prevention
Laboratory Training Branch is approved as a provider of
continuing education programs in the clinical laboratory
sciences by the ASCLS P.A.C.E.® Program. This course is
approved for 1.5 contact hours.
This course has been approved for 1.5 contact hours in
the category of Microbiology/Mycology/Parasitology
for Florida Laboratory Licensees.

OBJECTIVES
AUDIENCE
Laboratory professionals who have knowledge of basic
medical microbiology and who perform antimicrobial
susceptibility testing or are considering adding it to the
laboratory's testing menu.

At the conclusion of this program, the participant will
be able to:




SPECIAL NEEDS
Course content is closed captioned where applicable and
optimized for a screen reader.




Identify the steps required to perform the
clinical and Laboratory Standard Institute (CLSI)
disk diffusion and Minimal Inhibitory
Concentration (MIC) reference procedures.
Identify the key steps that must be modified
when performing disk diffusion or MIC
procedures for fastidious organisms.
Recognize CLSI's standards on commercial
antimicrobial susceptibility test systems.
Review the importance and limitations of
commercial antimicrobial susceptibility test
systems.

FREE REGISTRATION
·
·
·

Register online at www.cdc.gov/labtraining
If you have difficulty with the online registration
process, please email [email protected]
For additional program information, email
[email protected] or call (404) 498-6022

CE INFORMATION
P.A.C.E.™ Course Number: 288-023-17
FL Course Number: 20-413202

For a complete list of courses, visit www.cdc.gov/labtraining.

Online Course

MASTER: Antimicrobial Susceptibility CLSI
Standards
Sponsored by the Centers for Disease Control and Prevention Office of Antimicrobial Resistance

| DESCRIPTION |

| REGISTRATION |

This course provides a detailed overview of the Clinical
and Laboratory Standards Institute (CLSI) standards for
antimicrobial susceptibility testing of bacteria isolated
from clinical specimens. It focuses on the supplemental
tables that correlate with the standards and the
interpretive criteria for antimicrobials recommended for
testing a variety of bacteria. The course provides
essential information for those new to antimicrobial
susceptibility testing and serves as an important
refresher for experienced laboratory professionals.

FREE REGISTRATION

| AUDIENCE |
Laboratory professionals who have knowledge of basic
medical microbiology and who perform antimicrobial
susceptibility testing or are considering adding it to the
laboratory’s testing menu.

| OBJECTIVES |
At the conclusion of this program, the participant will be
able to:
 Recognize the role of CLSI in developing standards for

 Register online at www.cdc.gov/labtraining
 If you have difficulty with the online registration process,

please email [email protected]
 For additional program information, email
[email protected] or call (404) 498-6022

| CONTINUING EDUCATION |
The Centers for Disease Control and Prevention Laboratory
Training Branch is approved as a provider of continuing
education programs in the clinical laboratory sciences by
the ASCLS P.A.C.E.® Program. This course is approved for
1.0 contact hours.
This course has been approved for 1.0 contact hours in the
category of Microbiology/Mycology/Parasitology for Florida
Laboratory Licensees.

| SPECIAL NEEDS |
Course content is closed captioned where applicable and
optimized for a screen reader.

antimicrobial susceptibility testing of bacteria
isolated from clinical specimens.
 Identify CLSI standards for antimicrobial susceptibility

testing of bacteria isolated from clinical specimens.
 Given a particular organism, select antimicrobial

agents appropriate for testing and reporting from
the correct CLSI table.
 Outline a strategy for selective reporting of

antimicrobial agents using CLSI tables.
P.A.C.E.® Course Number: 288-025-17
FL Course Number: 20-454342

For a complete list of courses, visit www.cdc.gov/labtraining.