NIST 2nd Interlaboratory Comparison Excercise for Lipidomics and Metabolomics Collection

OMB Control #0693-0033
Expiration Date: 06/30/2019
The purpose of this survey is to collect information, from the community, regarding potential
reference grade tissue materials (e.g., tissues offered, what species, and how much tissue should
we provide per vial). We want to make sure to provide materials that have value to the community
and will be useful.
In addition, using the developed reference grade materials, we also aim to conduct an
interlaboratory comparison exercise for both lipids and metabolites. The core interlaboratory
exercise will be to analyze, in triplicate, two prototype tissue-based reference grade materials (brain
and liver) for lipids, metabolites, and/or both (whatever your laboratory routinely measures).
Several questions below will let us know your interest in these activities, as well as provide
feedback regarding how the exercises are designed.
1. PI name (none of the responses will be made public, this is just so we can follow-up with interested
laboratories)

2. For the healthy brain tissue reference grade material, what species do you find ideal?
Mouse
Rat
Pig
Human
All listed are suitable
Other (please specify)

3. How much brain tissue (in mg) do you use (or would anticipate using) per single lipidomic/metabolomic
analysis? We ask this question to help us determine the amount of tissue we should provide per vial.

4. Would you find it useful to have a diseased brain tissue material as well?
Tumor
Dementia or Alzheimer's
No Interest
Other (please specify)

5. For healthy liver tissue reference grade material, what species do you find ideal?
Mouse

Bovine

Rat

Human

Pig

All are suitable

Other (please specify)

6. How much liver tissue (in mg) do you use (or would anticipate using) per single lipidomic/metabolomic
analysis?

7. Would you find it useful to have a diseased liver tissue as well? If so, which condition?
Yes
No
Other (please specify)

8. Is there another tissue you think we should also offer?

9. Do you have a preference as to how you would like the reference grade tissue materials offered?
Frozen powdered tissue
Lyophilized tissue
Both
Other (please specify)

10. Do you find any value in NIST offering pre-extracted tissue lipid/metabolite materials as well?
Yes
No
Other (please specify)

* 11. Are you interested in participating in an interlaboratory exercise focused on evaluating these tissue
materials (quantitatively)? Note that while your answer is not binding, by selecting yes, you agree to receive
updates regarding the initiation of this exercise.
Yes
No

12. If yes, what will your laboratory measure?
Lipids
Metabolites
Both

13. How long would you need to make triplicate measurements of these tissue reference materials? Note
that we are anticipating/projecting to have the materials ready to distribute by the beginning of 2018.

14. Would you also be interested in participating in a longitudinal study using these tissue materials? To
evaluate the effectiveness of these reference grade materials over time, we are asking for a select number
of laboratories to be willing to make triplicate measurements of these materials once per year for 3 years.
Yes
No
Maybe

15. Other activities are planned for the upcoming year and beyond. Please let us know your interest, if any.
If interested, we will put you on our list and you will receive updates on the progress of these potential
activities. Select those that you are interested in (more than one option may apply)
Create reference materials for emerging matrices (saliva, sputum, feces, bronchoalveolar lavage, bile, cerebrospinal fluid).
Create a reference material for dried blood spot cards and perform a follow-up interlaboratory exercise.
Evaluate a NIST plasma SRM using a set/specific internal standard mixture and/or lipidomic/metabolomic workflow.
Interlaboratory exercise focused on evaluating lipidomic/metabolomic data

16. For the laboratories that perform metabolomics, would you be interested in quantitatively analyzing the
metabolome of SRM 1950 (in triplicate)? Or if you have already analyzed SRM 1950 in this manner, would
you be willing to provide us your data (and/or concentration values) and methods? The intention of this
request, is to calculate consensus means with associated uncertainties for the metabolome in SRM 1950,
similar to the lipidomic quantitative analysis of this material. The community-derived consensus means
(using submitted data) will be publicly accessible and could serve as benchmarks to promote harmonization
within the community.
Yes, I would be willing to analyze the metabolome of SRM 1950 (providing concentration values in triplicate and method
information)
Yes, I have quantitative metabolomic data for SRM 1950 that I would be willing to send (concentration values from a triplicate
analysis) along with method information
Yes, I have metabolomic QC data for SRM 1950 that I would be willing to send with methods
No, I do not want to participate

This collection of information contains Paperwork Reduction Act (PRA) requirements approved by the Office of Management and
Budget (OMB). Notwithstanding any other provisions of the law, no person is required to respond to, nor shall any person be subject to
a penalty for failure to comply with, a collection of information subject to the requirements of the PRA unless that collection of
information displays a currently valid OMB control number. Public reporting burden for this collection is estimated to be 7 minutes total,
including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed and
completing and reviewing the collection of information. Send comments regarding this burden estimate or any aspect of this collection
of information, including suggestions for reducing this burden, to the National Institute of Standards and Technology, Attn: John A.
Bowden ([email protected]).
OMB Control No. 0693-0033
Expiration Date: 06/30/2019