VS 21-206 2020 NAHMS Swine Large Enterprise - Saliva Collection Fo

According to the Paperwork Reduction Act of 1995, no persons are required to respond to a collection of information
unless it displays a valid OMB control number. The valid OMB control number for this information collection is 05790315. The time required to complete this information collection is estimated to average 2 hours per response,
including the time to review instructions, search existing data resources, gather the data needed, and complete and
review the information collected.
UNITED STATES DEPARTMENT OF AGRICULTURE
ANIMAL AND PLANT HEALTH INSPECTION SERVICE
VETERINARY SERVICES
NATIONAL ANIMAL HEALTH MONITORING SYSTEM
2150 CENTRE AVE, BLDG B
FORT COLLINS, CO 80526

OMB Approved
0579-0315
EXP: XX/20XX

Swine 2020 Large Enterprise
Saliva Collection Record

Kit Contents
•
•
•
•
•

30 feet of 5/8" 3 strand cotton rope
10 quart Ziploc® bags
1 gallon Ziploc® bag
10, 50 ml centrifuge tubes
Insulated cooler and shipping box

•
•
•
•

3 ice packs (freeze prior to farm visit)
1 absorbent sheet
2 linear bags (one used as trash bag, one used to
line the cooler)
Paperwork packet: Oral Fluids Collection Record,
10 centrifuge tubes labels, shipping airbill

Collection Instructions
Collect samples on a Sunday-Thursday. It is important that the site house 20 weeks and older finishing pigs in a
barn or enclosure (i.e., a hoop barn) in pens. Hang ropes in a maximum of 8 pens in one barn or room within a
barn in pens populated by these pigs. If one barn or room contains the site’s oldest finishing pigs, pick that one.
Otherwise take any barn that is available with the right age and type of pigs. The ropes should be hung in pens that are
roughly equidistant from one another.
Ropes are hung by taking one end of the cotton rope (5/8" 3 Strand Cotton) and tying one end to the top bar of each
pen or the gate. Tie the rope knot on the gate and cut the end in the pen so that that this end hangs approximately at
shoulder height for most of the animals in the pen. Unravel the part of the rope that hangs in the pen rope into its
strands. Shake the end of the rope in the pen so that pigs are attracted to it.
Wait 30-45 minutes while they chew on the ropes. While the rope is still hanging, insert the lower, wet portion of each
rope into a quart sized Ziploc® bag and cut the part that’s inside the bag from the remainder of the hanging rope.
Manually squeeze the rope pieces while inside the Ziploc® bag to release the oral fluid. Cut the corner of the Ziploc®
bag and let the fluid drip into a 50 ml centrifuge tube. Close each centrifuge tube, checking that the lid is tight. Place the
used Ziploc® bags and the rope ends in the trash bag. Untie and place excess hanging rope in the trash bag. Write the
8-digit Farm ID (State/operation/site), Facility, and Pen IDs on a sample label, peel off and place securely on the
appropriate centrifuge tubes.
Place centrifuge tubes in the gallon sized Ziploc® plastic bag and seal. Place the Ziploc® bag inside the liner bag with 3
frozen ice packs and the absorbent sheet. Place the liner bag inside the insulated shipment container and close the
shipping container lid.

Collection Record Instructions
Complete the Oral Fluids Collection Record Form (attached below). Place the original (white) collection record on
top of the insulated cooler box lid. Leave the yellow copy of the collection record with the producer. Secure the
shipping box.

Shipping and Results
Keep samples cool in a refrigerator, attach shipping airbill (addressed to ISU) to the shipping box, and ship via Fed Ex
within 24 hours of collection on Monday-Thursday. The samples will be tested for PRRS, Flu, SVA and three
types of Coronaviruses and results will be returned to the Producer in approximately 45-60 days after collection.

VS Form 21-206
June 2020

1

Swine 2020: Oral Fluids Collection Record
Farm ID

8-digits: St, Op, Site

# of People Involved
Key Collector
Initials
___ Fed VMO ___ Fed AHT
___ Producer ___ State VMO
___ St AHT
___ Priv Vet
___ Others-specify:____________
Enter number for each category

Rope
Number Facility/Barn ID1 Pen ID1
1
2
3
4
5
6
7
8
1Any

Facility
Type2
T O
T O
T O
T O
T O
T O
T O
T O

Collection Date

MM/DD/YY

Approximate # of pigs
Where was # of pigs in share air
rope hung?3
pen
space4
B G O
B G O
B G O
B G O
B G O
B G O
B G O

Hours to Take
and Prep
Samples

In quarter hours

Average age of
pigs in pen
(weeks)

Hours of Overall
Travel Time

In quarter hours

Kit Number

Must match labels

Vaccination codes6
Gender of pigs in pen5
G B M
G B M
G B M
G B M
G B M
G B M
G B M

B G O

1st

2nd 3rd 4th

G B M

designation will do whether text or numeric as long as it differentiates between row entries.

2T=Total

Confinement, O=Open building. Total confinement means solid exterior walls and mechanical ventilation. An open building generally has open sides or the potential for
sides to be opened weather permitting.

3B=Bar
4This

(e.g., top bar of pen enclosure), G=Gate, O=Other

would be the number of pigs in the barn or room within a barn usually.
B=Barrows and M=Mixed.

5G=Gilts,
6Up

to 4 different vaccinations may be entered. The codes and what they stand for are listed below. These are vaccinations that may have been given at any time in the pigs’ lives.

1=APP (Actinobacillus pleuropneumoniae)
2=Actinobacillus suis (autogenous)
3=Atrophic rhinitis (Bordatella/Pasteurella)
4=Clostridium difficile (autogenous)
5=Clostridium perfringens Type A
6=Clostridium perfringens Types C and D
7=Erysipelas
8=E. coli (K88, K99, 987P, F41)
9=Glasser's disease (Haemophilus parasuis)
10=Ileitis (Lawsonia intracellularis)
VS Form 21-206
June 2020

Vaccination codes

11=Influenza
12=Leptospirosis
13=Mycoplasma hyopneumoniae
14=Porcine circovirus 2
15=PRRS
16=Porcine epidemic diarrhea
17=Rotavirus
18=Salmonella
19=Streptococcus suis
20=TGE (transmissible gastroenteritis)
2