Form 0920-0978 2020 Laboratory Testing Practices for Candidemia Questio

2020 LABORATORY TESTING PRACTICES FOR CANDIDEMIA QUESTIONNAIRE

Form approved
OMB No. 0920-0978
Expires xx/xx/xxxx

1) What kind of laboratory is this facility? (select one)
Hospital laboratory
Other (specify) ______________________
Commercial laboratory (Quest, etc.)
Unknown
2) Does this facility ever receive blood cultures from nursing homes or other long term care facilities?
Yes
No
Unknown
3) Does the clinical microbiology laboratory at your institution have a separate Mycology section or laboratory?
Yes
No
Unknown
4) What kind of blood culture system does your laboratory use? (check all that apply)
BacT/Alert
Bactec FX
BacT/Alert 3D
Isolator tubes
VersaTREK
Other (specify) ______________________
Bactec 9240
5) What is the approximate volume of any type of fungal cultures performed annually in your laboratory?
Specify number: ______________
Unknown
6) What is the approximate volume of fungal cultures from blood performed annually in your laboratory?
Specify number: ______________ Unknown
7) How does your lab identify yeast? (check all that apply)
MALDI-TOF Bruker (Biotyper)
MALDI-TOF bioMerieux (VITEK MS)
VITEK 2
API 20C
DNA sequencing
PNA-FISH

BactiCard Candida
BD Phoenix
MicroScan
RapID Plus
Other (specify) ______________________
Unknown

8) Does your laboratory routinely use Chromagar for the identification or differentiation of Candida isolates?
Yes
No
Unknown
9) Species-level identification is performed for Candida spp. isolated from which of the following?
a. Blood isolates
Yes, reflexively

Yes, with clinician order

No

Unknown

b. Other normally sterile body site isolates
Yes, reflexively
Yes, with clinician order

No

Unknown

c. Abdominal isolates
Yes, reflexively

Yes, with clinician order

No

Unknown

d. Respiratory isolates
Yes, reflexively

Yes, with clinician order

No

Unknown

e. Urine isolates
Yes, reflexively

Yes, with clinician order

No

Unknown

Other (specify) ____________________
Yes, reflexively
Yes, with clinician order

No

Unknown

f.

Public reporting burden of this collection of information is estimated to average 10 minutes for the entire survey, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the
data needed, and completing and reviewing the collection of information. An agency may not conduct or sponsor, and a person is not required to respond to a collection of information unless it displays a currently valid OMB
Control Number. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden to CDC/ATSDR Information Collection Request Office,
1600 Clifton Road NE, MS D-74, Atlanta, Georgia 30333; ATTN: PRA (0920-0978).

Lab ID No. [][][][][][]
10) Does your laboratory employ the T2Candida Panel to identify Candida from blood specimens?
Yes (got to 10a)
No (go to 11)
Unknown
a. If Yes, when did this lab first start using T2Candida Panel? Date (mm/dd/yyyy): ____/____/________
b. If Yes, does this lab culture blood if you get a positive result on T2Candida Panel?
Yes, reflexively
No
Yes, with a clinical order
Unknown
11) Does your laboratory employ the BioFire (FilmArray) to identify Candida from blood culture?
Yes (go to 11a)
No (go to 12)
Unknown
a. If Yes, when did this lab first start using BioFire? Date (mm/dd/yyyy): ____/____/________
b. If Yes, does this lab reflexively culture blood if you get a positive result on BioFire?
Yes, reflexively
No
Yes, with a clinical order
Unknown
12) If No for both Question 10 and 11, does your laboratory have plans to employ culture-independent
diagnostics for Candida identification in the near future (e.g. T2Candida Panel, BioFire)?
Yes
Unknown
No
Not applicable (Yes to Q17 or Q18)
13) Does your laboratory offer any antifungal susceptibility testing for Candida?
Yes (Continue onto Page 2)
No (------ If No, QUESTIONNAIRE COMPLETE --------)
Unknown (is there another laboratory staff member who can assist with the questionnaire?)

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Lab ID No. [][][][][][]
ANTIFUNGAL SUSCEPTIBILITY TESTING
14) Where is antifungal susceptibility testing (AFST) done? (check all that apply)
On-site, in the laboratory
Sent to commercial lab
Sent to affiliated hospital lab
Other ______________________________
Unknown
15) Is antifungal susceptibility testing available for any of the following antifungal drugs (check all that apply):
Fluconazole
Caspofungin
Voriconazole
Amphotericin B
Itraconazole
Flucytosine
Posaconazole
Other (specify) __________________
Micafungin
Unknown
Anidulafungin
16) What methods are used for AFST? (check all that apply)
Non-commercial broth microdilution
YeastOne
E test

Vitek
Other ______________________________
Unknown

a. If you use Vitek for AFST, what Candida species do you test with it? (check all that apply)
C. albicans
C. parapsilosis
C. glabrata
Other Candida spp.
17) How are results of AFST reported? (select one)
Categorical interpretation only (susceptible,
resistant, etc.)
MIC only

Both--categorical interpretation PLUS MIC
Unknown

a. If categorical interpretation only, how do you determine the categorical interpretation? (check all that
apply)
CLSI M27 S4
Apply epidemiologic breakpoints
CLSI M27 S3
Other ______________________________
From manufacturer of MIC test
18) For what type of Candida isolates is antifungal susceptibility testing (AFST) performed
automatically/reflexively? (check all that apply)
Blood isolates
No AFST performed automatically (requires
Other normally sterile body site isolates
order from a clinician)
Other (specify) ______________________
Unknown

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Lab ID No. [][][][][][]
19) How is AFST performed for the following Candida spp.?
a. C. albicans
Performed automatically/reflexively (Go to 19ai)
Performed with a clinician’s order
Not performed
i.

Drugs for which AFST is performed automatically/reflexively on C. abicans (check all that apply):
Micafungin
Fluconazole
Other
Anidulafungin
Voriconazole
Unknown
Caspofungin
Amphotericin B

b. C. glabrata
Performed automatically/reflexively (Go to 19bi)
Performed with a clinician’s order
Not performed
i.

Drugs for which AFST is performed automatically/reflexively on C. glabrata (check all that apply):
Micafungin
Fluconazole
Other
Anidulafungin
Voriconazole
Unknown
Caspofungin
Amphotericin B

c. C. parapsilosis
Performed automatically/reflexively (Go to 19ci)
Performed with a clinician’s order
Not performed
i.

Drugs for which AFST is performed automatically/reflexively on C. parapsilosis (check all that
apply):
Micafungin
Fluconazole
Other
Anidulafungin
Voriconazole
Unknown
Caspofungin
Amphotericin B

d. Other Candida spp.
Performed automatically/reflexively (Go to 19di)
Performed with a clinician’s order
Not performed
i.

Drugs for which AFST is performed automatically/reflexively on other Candida spp. (check all that
apply):
Micafungin
Fluconazole
Other
Anidulafungin
Voriconazole
Unknown
Caspofungin
Amphotericin B

------------------------------------------------ END OF QUESTIONNAIRE ------------------------------------------------

2020

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