VS 21-206 2021 NAHMS Swine Large Enterprise - Saliva Collection Fo

Animal and Plant
Health
Inspection Service
Veterinary
Services

NAHMS Swine 2021
Oral Fluids
Instructions

National Animal Health
Monitoring System
2150 Centre Ave, Bldg B
Fort Collins, CO 80526

Kit Contents
• 32 Feet of 5/8" 3 strand cotton rope
• 10 Quart Ziploc® bags
• 8 pairs of gloves
• 1 Gallon Ziploc® bag
• 8, 50 ml Centrifuge tubes with a box
• Insulated cooler and shipping box
• Rope Cutters

• 3 Ice packs (freeze prior to farm visit)
• 1 Absorbent sheet
• 2 Linear bags (one used as trash bag, one used to line
the cooler)
• Paperwork packet: Oral Fluids Collection Record, 10
centrifuge tubes labels, shipping airbill

Collection Instructions
Collect samples on a Sunday-Wednesday. The site must house 1 or more finishing pigs (20 weeks or older)
housed in a barn or enclosure (i.e. a hoop barn) in pens. Hang ropes in a maximum of 8 pens in a barn(s) or room
within a barn in pens populated by these pigs. If one barn or room contains the site’s oldest finishing pigs, pick that one.
The ropes should be hung in pens that are roughly equidistant from one another.
Ropes are hung by taking one end of the cotton rope (5/8" 3 Strand Cotton) and tying one end to the top bar of each pen
or the gate. Tie the rope knot on the gate and cut the end in the pen so that that this end hangs approximately at shoulder
height for most of the animals in the pen. Unravel the part of the rope that hangs in the pen rope into its strands. Shake
the end of the rope in the pen so that pigs are attracted to it.
Wait 30-45 minutes while they chew on the ropes. While the rope is still hanging, insert the lower, wet portion of each rope
into a quart sized Ziploc® bag and cut the part that’s inside the bag from the remainder of the hanging rope. Manually
squeeze the rope pieces while inside the Ziploc® bag to release the oral fluid. Cut the corner of the Ziploc® bag and let
the fluid drip into a 50 ml centrifuge tube. You only need to collect 25ml of fluid, so only fill the tube half full. Close
each centrifuge tube, checking that the lid is tight. Place the used Ziploc® bags and the rope ends in the trash bag. Untie
and place excess hanging rope in the trash bag. Write the 6-digit Farm ID (operation-site), Facility, and Pen IDs on a
sample label, peel off and place securely on the appropriate centrifuge tubes. Place the centrifuge tubes in a centrifuge
tube box.
Place boxed centrifuge tubes in the gallon sized Ziploc® plastic bag and seal. Place the Ziploc® bag inside the liner bag
with 3 frozen ice packs and the absorbent sheet. Place the liner bag inside the insulated shipment container and close the
shipping container lid.

Collection Record Instructions
Complete the Oral Fluids Collection Record Form (attached below). Skip the first row that is labeled “lab use only.” Place
the original (white) collection record on top of the insulated cooler box lid. Leave the yellow copy of the collection
record with the producer. Secure the shipping box.

Shipping and Results
Keep samples cool in a refrigerator, attach shipping airbill (addressed to ISU) to the shipping box, and ship within 24
hours of collection on Sunday-Thursday. The samples will be tested for the nucleic acid of Seneca Valley virus and
results will be returned to the Producer in approximately 90 days after collection.
According to the Paperwork Reduction Act of 1995, an agency may not conduct or sponsor, and a person is not required to respond
to a collection of information unless it displays a valid OMB control number. The valid OMB control number for this information
collection is 0579-0315. The time required to complete this information collection is estimated to average 2 hour per response,
including the time to review instructions, search existing data resources, gather the data needed, and complete and review the
information collected.

OMB Approved
0579-0315
EXP: 01/2023

Swine 2021: Oral Fluids Collection Record

State Coordinator Name: __________________________

Submitter: NAHMS

Reference: Use Kit Number

(lab use only)

Farm ID

6-digits: Op, Site

Owner: Swine 2021
(lab use only)

1Any

(lab use only)

# of People Involved
Key Collector
Initials
___ Fed VMO ___ Fed AHT
___ Producer ___ State VMO
___ St AHT
___ Priv Vet
___ Others-specify:____________
Enter number for each category

Rope
Number Facility/Barn ID1 Pen ID1
1
2
3
4
5
6
7
8

Site: Use Farm ID

Facility
Type2
T O
T O
T O
T O
T O
T O
T O
T O

(lab use only)

Collection Date

MM/DD/YY

Approximate # of pigs
Where was # of pigs in share air
rope hung?3
pen
space4
B G O
B G O
B G O
B G O
B G O
B G O

Hours to Take
and Prep
Samples

In quarter hours

Average age of
pigs in pen
(weeks)

Animal ID: Use Barn ID
(lab use only)

Hours of Overall
Travel Time

In quarter hours

Kit Number

Must match labels

Vaccination codes6
Gender of pigs in pen5
G B M
G B M
G B M
G B M
G B M
G B M

B G O
B G O

1st

2nd 3rd 4th

G B M
G B M

designation will do whether text or numeric as long as it differentiates between row entries.

2T=Total

Confinement, O=Open building. Total confinement means solid exterior walls and mechanical ventilation. An open building generally has open sides or the potential for
sides to be opened weather permitting.

3B=Bar
4This

(e.g., top bar of pen enclosure), G=Gate, O=Other

would be the number of pigs in the barn or room within a barn usually.
B=Barrows and M=Mixed.

5G=Gilts,
6Up

to 4 different vaccinations may be entered. The codes and what they stand for are listed below. These are vaccinations that may have been given at any time in the pigs’ lives.

Vaccination codes

1=APP (Actinobacillus pleuropneumoniae)
2=Actinobacillus suis (autogenous)
3=Atrophic rhinitis (Bordatella/Pasteurella)
4=Clostridium difficile (autogenous)
5=Clostridium perfringens Type A
6=Clostridium perfringens Types C and D
7=Erysipelas
8=E. coli (K88, K99, 987P, F41)
9=Glasser's disease (Haemophilus parasuis)
10=Ileitis (Lawsonia intracellularis)

11=Influenza
12=Leptospirosis
13=Mycoplasma hyopneumoniae
14=Porcine circovirus 2
15=PRRS
16=Porcine epidemic diarrhea
17=Rotavirus
18=Salmonella
19=Streptococcus suis
20=TGE (transmissible gastroenteritis)