VS 21-322 Bison 2022 Study Enteric Microbe Collection Record

According to the Paperwork Reduction Act of 1995, no persons are required to respond to a collection of information unless it displays a valid OMB control number. The valid OMB control number for this information collection is 0579-0420. The time required to complete this information collection is estimated to average 2 hours 30 minutes per response, including the time to review instructions, search existing data resources, gather the data needed, and complete and review the information collected.		OMB Approved 0579-0420 EXP: XX/XXXX
UNITED STATES DEPARTMENT OF AGRICULTURE ANIMAL AND PLANT HEALTH INSPECTION SERVICE VETERINARY SERVICES NATIONAL ANIMAL HEALTH MONITORING SYSTEM 2150 CENTRE AVE, BLDG B FORT COLLINS, CO 80526	NAHMS Bison 2022 Enteric Microbe Collection Record

Collect the enteric microbe fecal sample at the same time as the pre-deworming Kit A samples
(2 fecal samples per bison, but ship the samples in their respective kits; they will be shipped to different labs). Enteric Microbe samples will be shipped to NCSU for testing. You will need to ship these samples within 24 hours of collection. Collect and ship samples Sunday through Wednesday.

NUMBER OF BISON TO SAMPLE

Number of bison on an Operation	Sample Number
1-9 bison	Sample all bison
10-24 bison	Sample up to 15 bison
25-99 bison	Sample up to 25 bison
100 or more bison	Sample up to 30 bison

Select animals that will be representative of the herd. If possible, include pregnant/nursing bison as well as young bison in your sample collection.

Step-by-Step Guide to Sample Collection

Please follow the instructions provided below for collection and submission of samples. Two samples per Animal (duplicate samples) need to be submitted to complete both the fecal parasite testing and the enteric microbe testing. The pre-deworming fecal parasite Kit A samples will be submitted in the box labeled FP Kit A. The Enteric Microbe samples will be submitted in the Enteric Microbe (EM Kit) box.

1. Freeze ice packs 24 hours ahead of time.

2. Turn a Whirl-Pak® bag inside out over your hand.

3. Pick up a small handful (golf ball size) of fresh fecal material (not petrified) from the top of the pile to prevent contamination from the ground. One fecal ball per bag is adequate. Include diarrhea samples.

4. If possible, associate the sample with a specific animal. If you cannot identify specific animal samples, please provide a pasture ID and describe the group of animals that the sample is from in the collection form row for that group. Include information on gender, age range, and breed for the group.

5. Turn the bag right side out with your other hand and squeeze extra air out of the bag. Roll the bag twice and twist closed to prevent leakage.

6. Place a preprinted label on the sample bag. Do not use the label to seal the bag. Write the Bison Unique ID and Farm ID on each label. Write the Bison Unique ID in the corresponding row for Bison sample # on the table in Enteric Microbe Form.

REPEAT STEPS 2-6 for duplicate samples if submitting Pre-Deworming Fecal Parasite samples. Use bags and labels from the Enteric Microbe kit (EM kit) for enteric microbe sampling. Use bags and labels from the Pre-Deworming Fecal Parasite Kit (FP Kit A) for fecal parasite sampling. The Fecal Parasite samples will be submitted in the FP Kit A. DO NOT INCLUDE FECAL PARASITE samples in the ENTERIC MICROBE KIT.

7. Cool samples down as soon as possible. Refrigerate until they are shipped. Do not freeze.

8. The Enteric Microbe Form is printed on 2-part carbonless paper. Please write firmly with a ballpoint pen and make sure the information appears clearly on both copies. The white copy goes with the samples to the lab. You may keep the yellow copy for your records.

Completing the table in Enteric Microbe Form (attached)

1. FARM ID: This ID will be filled out for you. Test results will refer to this unique ID number.

2. Kit ID: This number is printed on Enteric Microbe box and on the labels for the sample bags.

3. Collect the fecal samples at the same time you collect your pre-deworming fecal parasite samples.

4. Complete each column in the Enteric Microbe Form for each bison sampled. If you don’t know the answer, write DK. If you decline to answer, please draw a line through the cell so that we know you didn’t just forget to answer that question.

5. Place the white copy of the collection form on top of the Styrofoam lid and seal the box packing tape.

6. Keep the yellow copy of the collection form for your records.

7. Ship samples with the frozen ice pack no more than 24 hours after collecting the samples. Ship Sunday through Wednesday. Do not collect samples on Thursday-Saturday.

8. Use the enclosed preprinted, prepaid FedEx label addressed to NCSU

Contact FedEx to locate a convenient drop-off location that accepts priority overnight packages.

1–800–gofedex (1–800–463–3339) or www.fedex.com

Sample #	Bison unique ID*	Age (months OR years)	Gender (see codes below)	Breed- (see codes below)	Condition of fecal sample 1=Normal 2=Soft/cowpie-like 3=Watery, 4=bloody 5=Other (describe)	Did this animal receive individual antimicrobial therapy in the last 30 days? (Yes/No) [If No, SKIP next two columns]	Which individual antibiotic(s) were given in the last 30 days? [See reference card and enter code or specify “Other”]	What was the primary reason for the use of antibiotics in the last 30 days? NA=No Antibiotics used 1. Respiratory Disease 2. Pink Eye 3. Digestive 4. Reproductive 5. Lameness or injury 6. Other (specify)
1		___ mo or ___ yr
2		___ mo or ___ yr
3		___ mo or ___ yr
4		___ mo or ___ yr
5		___ mo or ___ yr
6		___ mo or ___ yr
7		___ mo or ___ yr
8		___ mo or ___ yr
9		___ mo or ___ yr
10		___ mo or ___ yr

*Any designation will do (text or numeric) as long as it identifies a specific animal (preferred) or group of animals.

Gender codes:

1 = Intact male 2 = Castrated male 3 = Intact female (nonpregnant) 4 = Pregnant female 5 = Spayed female 6 = Unknown status

Breed codes:

1 = Plains 2 = Wood 3 = Beefalo (hybrid) 4 = Wisent 5 = Zubron 6 = Other breed

Sample #	Bison unique ID*	Age (months OR years)	Gender (see codes below)	Breed- (see codes below)	Condition of fecal sample 1=Normal 2=Soft/cowpie-like 3=Watery, 4=bloody 5=Other (describe)	Did this animal receive individual antimicrobial therapy in the last 30 days? (Yes/No) [If No, SKIP next two columns]	Which individual antibiotic(s) were given in the last 30 days? [See reference card and enter code or specify “Other”]	What was the primary reason for the use of antibiotics in the last 30 days? NA=No Antibiotics used 1. Respiratory Disease 2. Pink Eye 3. Digestive 4. Reproductive 5. Lameness or injury 6. Other (specify)
11		___ mo or ___ yr
12		___ mo or ___ yr
13		___ mo or ___ yr
14		___ mo or ___ yr
15		___ mo or ___ yr
16		___ mo or ___ yr
17		___ mo or ___ yr
18		___ mo or ___ yr
19		___ mo or ___ yr
20		___ mo or ___ yr

*Any designation will do (text or numeric) as long as it identifies a specific animal (preferred) or group of animals.

Gender codes:

1 = Intact male 2 = Castrated male 3 = Intact female (nonpregnant) 4 = Pregnant female 5 = Spayed female 6 = Unknown status

Breed codes:

1 = Plains 2 = Wood 3 = Beefalo (hybrid) 4 = Wisent 5 = Zubron 6 = Other breed

Sample #	Bison unique ID*	Age (months OR years)	Gender (see codes below)	Breed- (see codes below)	Condition of fecal sample 1=Normal 2=Soft/cowpie-like 3=Watery, 4=bloody 5=Other (describe)	Did this animal receive individual antimicrobial therapy in the last 30 days? (Yes/No) [If No, SKIP next two columns]	Which individual antibiotic(s) were given in the last 30 days? [See reference card and enter code or specify “Other”]	What was the primary reason for the use of antibiotics in the last 30 days? NA=No Antibiotics used 1. Respiratory Disease 2. Pink Eye 3. Digestive 4. Reproductive 5. Lameness or injury 6. Other (specify)
21		___ mo or ___ yr
22		___ mo or ___ yr
23		___ mo or ___ yr
24		___ mo or ___ yr
25		___ mo or ___ yr
26		___ mo or ___ yr
27		___ mo or ___ yr
28		___ mo or ___ yr
29		___ mo or ___ yr
30		___ mo or ___ yr

*Any designation will do (text or numeric) as long as it identifies a specific animal (preferred) or group of animals.

Gender codes:

1 = Intact male 2 = Castrated male 3 = Intact female (nonpregnant) 4 = Pregnant female 5 = Spayed female 6 = Unknown status

Breed codes:

1 = Plains 2 = Wood 3 = Beefalo (hybrid) 4 = Wisent 5 = Zubron 6 = Other breed

VS Form 21-322 3

July 2022