BQASP Data Submission Form Phthalates

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Overview

Method Report
Results

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Sheet 1: Method Report

	Biomonitoring Quality Assurance Support Program
	Analytical Method Report
	Phthalates
	Laboratory Information
1	State Report:	Select State
2	Results for Method:	Select Method
	Sample Preparation Information:
3	Does your method use automation:	Yes or No
4	Does your sample preparation method include:	Select SPE
5	Solid phase extraction platform:	N/A
	Additional information on sample prep procedure:	Type description here
6	What is the method sample volume size:
7	Name of enzyme:
	Enzyme Vendor
	Enzyme Concentration		(please include units)
	Amount
	How long do you incubate the samples
	Temperature during incubations		Degrees Celsius
	HPLC Configuration:
8	Instrument manufacturer	Select	Type Other Here
9	What is the flow rate:
10	What is the method run time:
11	What is the sample injection volume:
12	Column name and Manufacture
13	Column dimensions
14	Elution Type:	Select
15	Mobile Phase A Composition		Describe composition for Mobile Phase A
16	Mobile Phase B Composition		Describe composition for Mobile Phase B
	Mass Spectrometer Configuration
17	Have you optimized the MS Parameters for your method? (Analytes, Precursor and Product Ions, Collision Energy)	Yes or No
18	What is the ionization mode:	Select Mode
20	Please complete the table for each analytes LOD, precursor and product ion transitions:	Analyte	LOD	Calibration Range	Precursor Ion (mass)	Product Ion (mass)
		Example: MCPP	0.4 ng/ml	0.035 - 350 ng/ml	251	103
		13C4-MCPP			225	103
	Analytical and Internal Standards
	Please complete the table for metabolite standards:	Analytical and Internal Standard	Vendor	Purity
		Phthalate Metabolites	Cambridge Isotope Laboratory
25	How do you prepare your standards?	Select
27	How many points are in the calibration curve?
28	Is the calibration curve weighted?		weighted curve: No weighting, 1/X, 1/X^2,Other ?
29	What integration software do you use?
	Additional Method Questions
30	Which proficiency testing programs do you participate in?	Select	Please type other programs here
31	what is the average number of samples analyzed per month for this method?
32	Have you checked the accuracy of the method using NIST SRMs?
33	What volume of sample is required for BQASP Analysis?
	Please provide a screenshot of your results chromatography:

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Sheet 2: Results

Phthalates
PT Event ID:	201902PHTU
Participant:
Analyst:
Reviewer:
Units of Result:
Sample ID	Analyte	Reported Value	Standard Deviation
201902001PHTU	mono-n-butyl phthalate
201902001PHTU	mono-3-carboxypropyl phthalate
201902001PHTU	monoethyl phthalate
201902001PHTU	mono-2-ethyl-5-carboxypentyl phthalate
201902001PHTU	monobenzyl phthalate
201902001PHTU	mono-2-ethylhexyl phthalate
201902001PHTU	mono-2-ethyl-5-hydroxyhexyl phthalate
201902001PHTU	mono-2-ethyl-5-oxohexyl phthalate
201902001PHTU	mono-isobutyl phthalate
201902001PHTU	mono-carboxyisooctyl phthalate
201902001PHTU	mono-carboxyisononyl phthalate
Sample ID	Analyte	Reported Value	Standard Deviation
201902002PHTU	mono-n-butyl phthalate
201902002PHTU	mono-3-carboxypropyl phthalate
201902002PHTU	monoethyl phthalate
201902002PHTU	mono-2-ethyl-5-carboxypentyl phthalate
201902002PHTU	monobenzyl phthalate
201902002PHTU	mono-2-ethylhexyl phthalate
201902002PHTU	mono-2-ethyl-5-hydroxyhexyl phthalate
201902002PHTU	mono-2-ethyl-5-oxohexyl phthalate
201902002PHTU	mono-isobutyl phthalate
201902002PHTU	mono-carboxyisooctyl phthalate
201902002PHTU	mono-carboxyisononyl phthalate
By submitting this form, we attest that the results reported were produced in this laboratory from the analysis of proficiency testing samples that were introduced into the routine workflow of the laboratory and analyzed using protocols and procedures with the same frequency routinely applied to patient specimens. We further attest that the laboratory did not discuss or engage in any communications with anyone outside of our laboratory regarding the proficiency test or the results obtained.