Form 1 Survey

Generic Clearance for the Collection of Qualitative Feedback on Agency Service Delivery (NIH)

BLAST Survey Instrument 5_8_2023

BLAST 2023 Survey (NLM)

OMB: 0925-0648

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BLAST 2023 Survey (NDB-406)
Start of Block: Default Question Block
OMB Control Number: 0925-0648
Expiration Date: 06/30/2024
Public reporting burden for this collection of information is estimated to average 8 minutes per
response, including the time for reviewing instructions, searching existing data sources,
gathering and maintaining the data needed, and completing and reviewing the collection of
information. An agency may not conduct or sponsor, and a person is not required to respond to,
a collection of information unless it displays a current valid OMB control number. Send
comments regarding this burden estimate or any other aspect of this collection of information,
including suggestions for reducing this burden, to NIH, Project Clearance Branch, 6705
Rockledge Drive, MSC 7974, Bethesda, MD 20892-7974, ATTN: PRA (0925-0648). Do not
return the completed form to this address.
All questions are optional, and you may exit the survey at any time.

Page 1 of 11

Which professional categories best describe you? Select all that apply.

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Bioinformatics professional
Educator
Healthcare professional
Librarian / Information Specialist
Life Science Researcher
Student
Technician
Computer Scientist / Software Developer

Other (please specify)
__________________________________________________

Page 2 of 11

Which of the following categories best describe your organization? Select all that apply.

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College or University
Commercial / Industry
Non-profit Organization
Research Institute
Government
Hospital/health organization

Other (please specify)
__________________________________________________

Page 3 of 11

How frequently do you use each of the following BLAST services on the web
(https://blast.ncbi.nlm.nih.gov/)?
Daily

Weekly

Monthly

Less than
monthly

Never

Nucleotide
BLAST
(blastn)

o

o

o

o

o

Protein
BLAST
(blastp)

o

o

o

o

o

o

o

o

o

o

o

o

o

o

o

o

o

o

o

o

PrimerBLAST

o

o

o

o

o

COBALT
(Constrainedbased
Multiple
Alignment
tool)

o

o

o

o

o

o
o

o
o

o
o

o
o

o
o

Translated
BLAST
(blastx,
tblastn,
tblastx)
SmartBLAST
Global Align
(align two or
more
sequence)

IgBLAST
MOLEBLAST

Page 4 of 11

Why do you use BLAST? Select all that apply.

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Searching unknown mRNA transcripts for functional identification
Searching unknown mRNA transcripts for taxonomic identification
Searching unknown proteins for functional identification
Searching unknown proteins for taxonomic identification
Annotating genes on genomic sequence
Search with known sequences to find homologs in other organisms
Identify organisms (taxa) in environmental/metagenomic samples
Screening sequences for contamination
Designing/Screening Primers
Assembling genome fragments
Education/Instruction

Other (please specify)
__________________________________________________

Page 5 of 11

How frequently do you search with multiple of sequences? Select all that apply.

o Daily
o Weekly
o Monthly
o Less than monthly
o Never
Which BLAST services do you use? Select all that apply.

▢

Use the BLAST+ applications on the command line remotely to submit BLAST
searches to NCBI

▢

Use the BLAST+ applications on the command line with my own/custom

databases

▢

Use the BLAST+ applications on the command line with BLAST
databases/FASTA provided by NCBI

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Submit BLAST searches using scripts that access the BLAST URL API
Use NCBI BLAST+ docker image from docker hub
Use BLAST on the cloud with a vendor (e.g AWS, GCP, etc).

Use BLAST through third-party software (please specify software used)
__________________________________________________

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N/A (I do not use any of these services)

Other (please specify)
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Page 6 of 11

How frequently do you use the standalone BLAST+ (command line) applications? Select all that
apply.

o Daily
o Weekly
o Monthly
o Less than monthly
o Never
Page Break

Why do you use the BLAST+ instead the BLAST web application?
________________________________________________________________
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Page 7 of 11

Please indicate how strongly you agree or disagree with the following statements about using
BLAST+.
Strongly
Disagree
I am satisfied
with the
performance
of BLAST+.
I frequently
look at the
databases
available to
see if there is
a better one
for me to use.
Available
formatting
options work
well for me.
I have an
automated
task that
downloads
the latest
databases.

Somewhat
disagree

Neither
agree nor
disagree

Somewhat
agree

Strongly
agree

o

o

o

o

o

o

o

o

o

o

o

o

o

o

o

o

o

o

o

o

What is one thing you would like to change in BLAST+?
________________________________________________________________
________________________________________________________________
________________________________________________________________
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Page 8 of 11

Please indicate how strongly you agree or disagree with the following statements about the
BLAST web application.
Strongly
Disagree
Knowing which
BLAST
databases to
choose is easy.

Somewhat
disagree

Neither
agree nor
disagree

Somewhat
agree

Strongly
agree

o

o

o

o

o

Finding BLAST
databases for a
specific
organism is
easy.

o

o

o

o

o

Finding BLAST
databases for a
specific
genome is
easy.

o

o

o

o

o

BLAST results
are returned
quickly.

o

o

o

o

o

BLAST results
are easy to
read and
understand.

o

o

o

o

o

Downloading
my results is
easy and
provides what I
need.

o

o

o

o

o

The
Descriptions
table has a
clear and
understandable
layout.

o

o

o

o

o

The
Descriptions
table has all
the fields I
need.

o

o

o

o

o
Page 9 of 11

What is one thing you would like to change about the BLAST web application?
________________________________________________________________
________________________________________________________________
________________________________________________________________
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What is the biggest challenge for you when you use BLAST?
________________________________________________________________
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Please enter your email below if you would like us to contact you for a BLAST user interview.
________________________________________________________________

If you would like to provide more feedback, please contact us at [email protected]

Page 10 of 11

How likely are you to recommend BLAST to a friend or colleague?

o0
o1
o2
o3
o4
o5
o6
o7
o8
o9
o 10
Page Break
End of Block: Default Question Block

Page 11 of 11


File Typeapplication/pdf
File TitleBLAST 2023 Survey (NDB-406)
AuthorQualtrics
File Modified2023-05-08
File Created2023-05-08

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