NCBI Crosswalk Data Dictionary

Attachment-06_Component-1_ref-NCBI_DCIPHER_Crosswalk_DataDictionary.xlsx

[NCEZID] National Wastewater Surveillance System for COVID-19 and other Infectious Disease Targets of Public Health Concern

NCBI Crosswalk Data Dictionary

OMB: 0920-1422

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Overview

Data crosswalk
NCBI sequencing data
NWSS wastewater data
NWSS wastewater data valuesets


Sheet 1: Data crosswalk

NCBI field NCBI description NWSS field
sample_name Sample Name is a name that you choose for the sample. It can have any format, but we suggest that you make it concise, unique and consistent within your lab, and as informative as possible. Every Sample Name from a single Submitter must be unique.
sample_title Title of the sample.
bioproject_accession The accession number of the BioProject(s) to which the BioSample belongs. If the BioSample belongs to more than one BioProject, enter multiple bioproject_accession columns. A valid BioProject accession has prefix PRJN, PRJE or PRJD, e.g., PRJNA12345.
organism The most descriptive organism name for this sample (to the species, if possible). It is OK to submit an organism name that is not in our database. In the case of a new species, provide the desired organism name, and our taxonomists may assign a provisional taxID. In the case of unidentified species, choose the appropriate Genus and include 'sp.', e.g., "Escherichia sp.". When sequencing a genome from a non-metagenomic source, include a strain or isolate name too, e.g., "Pseudomonas sp. UK4". For more information about providing a valid organism, including new species, metagenomes (microbiomes) and metagenome-assembled genomes, see https://www.ncbi.nlm.nih.gov/biosample/docs/organism/.
collection_date The date on which the sample was collected; date/time ranges are supported by providing two dates from among the supported value formats, delimited by a forward-slash character; collection times are supported by adding "T", then the hour and minute after the date, and must be in Coordinated Universal Time (UTC), otherwise known as "Zulu Time" (Z); supported formats include "DD-Mmm-YYYY", "Mmm-YYYY", "YYYY" or ISO 8601 standard "YYYY-mm-dd", "YYYY-mm", "YYYY-mm-ddThh:mm:ss"; e.g., 30-Oct-1990, Oct-1990, 1990, 1990-10-30, 1990-10, 21-Oct-1952/15-Feb-1953, 2015-10-11T17:53:03Z; valid non-ISO dates will be automatically transformed to ISO format sample_collect_date
geo_loc_name Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg "Canada: Vancouver" or "Germany: halfway down Zugspitze, Alps" wwtp_jurisdiction;
county_names;
zipcode
isolation_source Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived. sample_location
ww_population Number of persons contributing wastewater to this sample collection site; if unknown, estimate to the nearest order of magnitude, e.g., 10000. If no estimate is available, input NA. population_served
ww_sample_duration Duration of composite sample collected, in units of hours, e.g., 24. Specify integer values. If the sample is not a composite sample, use 0. composite_freq
ww_sample_matrix The wastewater matrix that was sampled sample_matrix
ww_sample_type Type of wastewater sample collected sample_type
ww_surv_target_1 Taxonomic name of the surveillance target. For the COVID-19 response, use 'SARS-CoV-2'. pcr_target
ww_surv_target_1_known_present Is genetic material of the surveillance target(s) known to the submitter to be present in this wastewater sample? Presence defined as microbiological evidence of the target organism in the wastewater sample, such as genetic- or culture-based detection.
collected_by Name of persons or institute who collected the sample
purpose_of_ww_sampling The reason the sample was collected
purpose_of_ww_sequencing The reason the sample was sequenced, e.g., identification of mutations within a specific region, presence of clinically known mutations, or diversity of mutations across entire genome
sequenced_by The name of the agency that generated the sequence, e.g., Centers for Disease Control and Prevention
ww_endog_control_1 The name of an organism, gene, or compound used as an endogenous wastewater control, e.g., pepper mild mottle virus hum_frac_target_mic;
hum_frac_target_chem;
other_norm_name
ww_endog_control_1_conc The concentration of the endogenous control specified in 'ww_endog_control_1' on a per wastewater unit basis, e.g., 700000000 hum_frac_mic_conc;
hum_frac_chem_conc;
other_norm_conc
ww_endog_control_1_protocol The protocol used to quantify 'ww_endog_control_1'. Specify a reference, website, or brief description. hum_frac_target_mic_ref;
hum_frac_target_chem_ref;
other_norm_ref
ww_endog_control_1_units The units of the value specified in 'ww_endog_control_1_conc', e.g., copies/L wastewater hum_frac_mic_unit;
hum_frac_chem_unit;
other_norm_unit
ww_endog_control_2 The name of an organism, gene, or compound used as an endogenous wastewater control, e.g., crassphage hum_frac_target_mic;
hum_frac_target_chem;
other_norm_name
ww_endog_control_2_conc The concentration of the endogenous control specified in 'ww_endog_control_2' on a per wastewater unit basis, e.g., 140000000 hum_frac_mic_conc;
hum_frac_chem_conc;
other_norm_conc
ww_endog_control_2_protocol The protocol used to quantify 'ww_endog_control_2'. Specify a reference, website, or brief description. hum_frac_target_mic_ref;
hum_frac_target_chem_ref;
other_norm_ref
ww_endog_control_2_units The units of the value specified in 'ww_endog_control_2_conc', e.g., copies/L wastewater hum_frac_mic_unit;
hum_frac_chem_unit;
other_norm_unit
ww_flow Daily volumetric flow through collection site, in units of liters per day, e.g., 110000000. flow_rate
ww_industrial_effluent_percent Percentage of industrial effluents received by wastewater treatment plant, e.g., 10 industrial_input
ww_ph pH measurement of the sample, or liquid portion of sample, or aqueous phase of the fluid, e.g., 7.2 ph
ww_population_source Source of value specified in 'ww_population', e.g., wastewater utility billing records, population of jurisdiction encompassing the wastewater service area, census blocks clipped to wastewater service area polygon
ww_pre_treatment Describe any process of pre-treatment that removes materials that can be easily collected from the raw wastewater, e.g., flow equilibration basin promotes settling of some solids if pretreatment == 'yes' then pretreatment_specify
ww_primary_sludge_retention_time The time primary sludge remains in tank, in hours, e.g., 4.
ww_processing_protocol The protocol used to process the wastewater sample. Processing includes laboratory procedures prior to and including nucleic acid purification (e.g., pasteurization, concentration, extraction, etc). Specify a reference, website, or brief description. solids_separation;
concentration_method;
extraction_method;
pasteurized
ww_sample_salinity Salinity is the total concentration of all dissolved salts in a liquid or solid (in the form of an extract obtained by centrifugation) sample or derived from the conductivity measurement (practical salinity) in milligrams per liter, e.g., 100. derived from conductivity
ww_sample_site The type of site where the wastewater sample was collected
Allowed values: correctional facility, long-term care facility, hospital, child day care, school, college or university, social services shelter, other residential building, ship, airplane, septic tank, other holding tank, wastewater treatment plant, wastewater lagoon, sewer pipeline, other, restricted access, not applicable, not collected, not provided, missing
institution_type
ww_surv_jurisdiction A jurisdiction identifer that can be used to support linking the sample to a public health surveillance system, e.g., va wwtp_jurisdiction
ww_surv_system_sample_id The sample ID used for submission to a public health surveillance system (e.g., CDC's National Wastewater Surveillance System), e.g., s123456 sample_id
ww_surv_target_1_conc The concentration of the wastewater surveillance target specified in 'ww_surv_target_1' on a per wastewater unit basis, e.g., 200000 pcr_target_avg_conc
ww_surv_target_1_conc_unit The units of the value specified in 'ww_surv_target_1_conc', e.g., copies/L wastewater pcr_target_units
ww_surv_target_1_extract Measured amount of surveillance target in the nucleic acid extract that was sequenced; on a per extract unit basis, rather than on a per wastewater sample unit basis, e.g., 100000
ww_surv_target_1_extract_unit The units of the value specified in 'ww_surv_target_1_extract', e.g., copies/microliter extract
ww_surv_target_1_gene The name of the gene quantified for the the surveillance target specified in 'ww_surv_target_1', e.g., N gene pcr_gene_target
ww_surv_target_1_protocol The protocol used to quantify 'ww_surv_target_1'. Specify a reference, website, or brief description. quant_stan_type;
stan_ref;
inhibition_method;
num_no_target_control;
pcr_gene_target_ref;
pcr_type
ww_surv_target_2 Taxonomic name of the surveillance target, eg, Norovirus pcr_target
ww_surv_target_2_conc The concentration of the wastewater surveillance target specified in 'ww_surv_target_2' on a per wastewater unit basis, e.g., 24000 pcr_target_avg_conc
ww_surv_target_2_conc_unit The units of the value specified in 'ww_surv_target_2_conc', e.g., copies/L wastewater pcr_target_units
ww_surv_target_2_extract Measured amount of surveillance target in the nucleic acid extract that was sequenced; on a per extract unit basis, rather than on a per wastewater sample unit basis, e.g., 12000
ww_surv_target_2_extract_unit The units of the value specified in 'ww_surv_target_2_extract', e.g., copies/microliter extract
ww_surv_target_2_gene The name of the gene quantified for the the surveillance target specified in 'ww_surv_target_2', e.g., ORF1-ORF2 junction pcr_gene_target
ww_surv_target_2_known_present Is genetic material of the surveillance target(s) known to the submitter to be present in this wastewater sample? Presence defined as microbiological evidence of the target organism in the wastewater sample, such as genetic- or culture-based detection.
ww_surv_target_2_protocol The protocol used to quantify 'ww_surv_target_2'. Specify a reference, website, or brief description. quant_stan_type;
stan_ref;
inhibition_method;
num_no_target_control;
pcr_gene_target_ref;
pcr_type
ww_temperature Temperature of the wastewater sample at the time of sampling in Celsius, e.g., 25. collection_water_temp
ww_total_suspended_solids Total concentration of solids in raw wastewater influent sample including a wide variety of material, such as silt, decaying plant and animal matter in milligrams per liter, e.g., 500. tss
description Description of the sample.

Sheet 2: NCBI sequencing data

NCBI field NCBI description NCBI required Notes
sample_name Sample Name is a name that you choose for the sample. It can have any format, but we suggest that you make it concise, unique and consistent within your lab, and as informative as possible. Every Sample Name from a single Submitter must be unique. Yes If information is unavailable for any mandatory field, please enter 'not collected', 'not applicable' or 'missing' as appropriate.
sample_title Title of the sample. No
bioproject_accession The accession number of the BioProject(s) to which the BioSample belongs. If the BioSample belongs to more than one BioProject, enter multiple bioproject_accession columns. A valid BioProject accession has prefix PRJN, PRJE or PRJD, e.g., PRJNA12345. No
organism The most descriptive organism name for this sample (to the species, if possible). It is OK to submit an organism name that is not in our database. In the case of a new species, provide the desired organism name, and our taxonomists may assign a provisional taxID. In the case of unidentified species, choose the appropriate Genus and include 'sp.', e.g., "Escherichia sp.". When sequencing a genome from a non-metagenomic source, include a strain or isolate name too, e.g., "Pseudomonas sp. UK4". For more information about providing a valid organism, including new species, metagenomes (microbiomes) and metagenome-assembled genomes, see https://www.ncbi.nlm.nih.gov/biosample/docs/organism/. Yes If information is unavailable for any mandatory field, please enter 'not collected', 'not applicable' or 'missing' as appropriate.
collection_date The date on which the sample was collected; date/time ranges are supported by providing two dates from among the supported value formats, delimited by a forward-slash character; collection times are supported by adding "T", then the hour and minute after the date, and must be in Coordinated Universal Time (UTC), otherwise known as "Zulu Time" (Z); supported formats include "DD-Mmm-YYYY", "Mmm-YYYY", "YYYY" or ISO 8601 standard "YYYY-mm-dd", "YYYY-mm", "YYYY-mm-ddThh:mm:ss"; e.g., 30-Oct-1990, Oct-1990, 1990, 1990-10-30, 1990-10, 21-Oct-1952/15-Feb-1953, 2015-10-11T17:53:03Z; valid non-ISO dates will be automatically transformed to ISO format Yes If information is unavailable for any mandatory field, please enter 'not collected', 'not applicable' or 'missing' as appropriate.
geo_loc_name Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg "Canada: Vancouver" or "Germany: halfway down Zugspitze, Alps" Yes If information is unavailable for any mandatory field, please enter 'not collected', 'not applicable' or 'missing' as appropriate.
isolation_source Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived. Yes If information is unavailable for any mandatory field, please enter 'not collected', 'not applicable' or 'missing' as appropriate.
ww_population Number of persons contributing wastewater to this sample collection site; if unknown, estimate to the nearest order of magnitude, e.g., 10000. If no estimate is available, input NA. Yes If information is unavailable for any mandatory field, please enter 'not collected', 'not applicable' or 'missing' as appropriate.
ww_sample_duration Duration of composite sample collected, in units of hours, e.g., 24. Specify integer values. If the sample is not a composite sample, use 0. Yes If information is unavailable for any mandatory field, please enter 'not collected', 'not applicable' or 'missing' as appropriate.
ww_sample_matrix The wastewater matrix that was sampled Yes If information is unavailable for any mandatory field, please enter 'not collected', 'not applicable' or 'missing' as appropriate.
ww_sample_type Type of wastewater sample collected Yes If information is unavailable for any mandatory field, please enter 'not collected', 'not applicable' or 'missing' as appropriate.
ww_surv_target_1 Taxonomic name of the surveillance target. For the COVID-19 response, use 'SARS-CoV-2'. Yes If information is unavailable for any mandatory field, please enter 'not collected', 'not applicable' or 'missing' as appropriate.
ww_surv_target_1_known_present Is genetic material of the surveillance target(s) known to the submitter to be present in this wastewater sample? Presence defined as microbiological evidence of the target organism in the wastewater sample, such as genetic- or culture-based detection. Yes If information is unavailable for any mandatory field, please enter 'not collected', 'not applicable' or 'missing' as appropriate.
collected_by Name of persons or institute who collected the sample No
purpose_of_ww_sampling The reason the sample was collected No
purpose_of_ww_sequencing The reason the sample was sequenced, e.g., identification of mutations within a specific region, presence of clinically known mutations, or diversity of mutations across entire genome No
sequenced_by The name of the agency that generated the sequence, e.g., Centers for Disease Control and Prevention No
ww_endog_control_1 The name of an organism, gene, or compound used as an endogenous wastewater control, e.g., pepper mild mottle virus No
ww_endog_control_1_conc The concentration of the endogenous control specified in 'ww_endog_control_1' on a per wastewater unit basis, e.g., 700000000 No
ww_endog_control_1_protocol The protocol used to quantify 'ww_endog_control_1'. Specify a reference, website, or brief description. No
ww_endog_control_1_units The units of the value specified in 'ww_endog_control_1_conc', e.g., copies/L wastewater No
ww_endog_control_2 The name of an organism, gene, or compound used as an endogenous wastewater control, e.g., crassphage No
ww_endog_control_2_conc The concentration of the endogenous control specified in 'ww_endog_control_2' on a per wastewater unit basis, e.g., 140000000 No
ww_endog_control_2_protocol The protocol used to quantify 'ww_endog_control_2'. Specify a reference, website, or brief description. No
ww_endog_control_2_units The units of the value specified in 'ww_endog_control_2_conc', e.g., copies/L wastewater No
ww_flow Daily volumetric flow through collection site, in units of liters per day, e.g., 110000000. No
ww_industrial_effluent_percent Percentage of industrial effluents received by wastewater treatment plant, e.g., 10 No
ww_ph pH measurement of the sample, or liquid portion of sample, or aqueous phase of the fluid, e.g., 7.2 No
ww_population_source Source of value specified in 'ww_population', e.g., wastewater utility billing records, population of jurisdiction encompassing the wastewater service area, census blocks clipped to wastewater service area polygon No
ww_pre_treatment Describe any process of pre-treatment that removes materials that can be easily collected from the raw wastewater, e.g., flow equilibration basin promotes settling of some solids No
ww_primary_sludge_retention_time The time primary sludge remains in tank, in hours, e.g., 4. No
ww_processing_protocol The protocol used to process the wastewater sample. Processing includes laboratory procedures prior to and including nucleic acid purification (e.g., pasteurization, concentration, extraction, etc). Specify a reference, website, or brief description. No
ww_sample_salinity Salinity is the total concentration of all dissolved salts in a liquid or solid (in the form of an extract obtained by centrifugation) sample or derived from the conductivity measurement (practical salinity) in milligrams per liter, e.g., 100. No
ww_sample_site The type of site where the wastewater sample was collected
Allowed values: correctional facility, long-term care facility, hospital, child day care, school, college or university, social services shelter, other residential building, ship, airplane, septic tank, other holding tank, wastewater treatment plant, wastewater lagoon, sewer pipeline, other, restricted access, not applicable, not collected, not provided, missing
No
ww_surv_jurisdiction A jurisdiction identifer that can be used to support linking the sample to a public health surveillance system, e.g., va No
ww_surv_system_sample_id The sample ID used for submission to a public health surveillance system (e.g., CDC's National Wastewater Surveillance System), e.g., s123456 No
ww_surv_target_1_conc The concentration of the wastewater surveillance target specified in 'ww_surv_target_1' on a per wastewater unit basis, e.g., 200000 No
ww_surv_target_1_conc_unit The units of the value specified in 'ww_surv_target_1_conc', e.g., copies/L wastewater No
ww_surv_target_1_extract Measured amount of surveillance target in the nucleic acid extract that was sequenced; on a per extract unit basis, rather than on a per wastewater sample unit basis, e.g., 100000 No
ww_surv_target_1_extract_unit The units of the value specified in 'ww_surv_target_1_extract', e.g., copies/microliter extract No
ww_surv_target_1_gene The name of the gene quantified for the the surveillance target specified in 'ww_surv_target_1', e.g., N gene No
ww_surv_target_1_protocol The protocol used to quantify 'ww_surv_target_1'. Specify a reference, website, or brief description. No
ww_surv_target_2 Taxonomic name of the surveillance target, eg, Norovirus No
ww_surv_target_2_conc The concentration of the wastewater surveillance target specified in 'ww_surv_target_2' on a per wastewater unit basis, e.g., 24000 No
ww_surv_target_2_conc_unit The units of the value specified in 'ww_surv_target_2_conc', e.g., copies/L wastewater No
ww_surv_target_2_extract Measured amount of surveillance target in the nucleic acid extract that was sequenced; on a per extract unit basis, rather than on a per wastewater sample unit basis, e.g., 12000 No
ww_surv_target_2_extract_unit The units of the value specified in 'ww_surv_target_2_extract', e.g., copies/microliter extract No
ww_surv_target_2_gene The name of the gene quantified for the the surveillance target specified in 'ww_surv_target_2', e.g., ORF1-ORF2 junction No
ww_surv_target_2_known_present Is genetic material of the surveillance target(s) known to the submitter to be present in this wastewater sample? Presence defined as microbiological evidence of the target organism in the wastewater sample, such as genetic- or culture-based detection. No
ww_surv_target_2_protocol The protocol used to quantify 'ww_surv_target_2'. Specify a reference, website, or brief description. No
ww_temperature Temperature of the wastewater sample at the time of sampling in Celsius, e.g., 25. No
ww_total_suspended_solids Total concentration of solids in raw wastewater influent sample including a wide variety of material, such as silt, decaying plant and animal matter in milligrams per liter, e.g., 500. No
description Description of the sample. No

Sheet 3: NWSS wastewater data

NWSS field NWSS description NWSS required Notes
reporting_jurisdiction The CDC Epidemiology and Laboratory Capacity (ELC) jurisdiction, most frequently a state, reporting these data (2-letter abbreviation) Yes
county_names 5-digit numeric FIPS codes of all counties and county equivalents served by this sampling site (i.e., served by this wastewater treatment plant or, if 'sample_location' is "upstream", then by this upstream location). Note that most jurisdictions are covered by counties, but some are covered by county equivalents, such as independent cities, parishes, or census areas. Yes
other_jurisdiction This field has been deprecated. Specify FIPS codes for counties and county equivalents in 'county_names'. No
zipcode ZIP code in which this sampling site is located Yes
population_served Estimated number of persons served by this sampling site (i.e., served by this wastewater treatment plant or, if 'sample_location' is "upstream", then by this upstream location) Yes
sewage_travel_time What is the approximate sewage travel time, on average, from sewage source to this sampling site (i.e., this wastewater treatment plant or, if 'sample_location' is "upstream", then this upstream location)? This should be specified as a duration in hours, not a time of day. No
sample_location Sample collection location in the wastewater system, whether at a wastewater treatment plant (or other community level treatment infrastructure such as community-scale septic) or upstream in the wastewater system Yes If 'sample_location' is "upstream", specify in 'sample_location_specify'
sample_location_specify If 'sample_location' is "upstream", specify the collection location in the wastewater system; an arbitrary name may be used if you do not wish to disclose the real name. Yes If sample_location is "upstream", then this must have a non-empty value
institution_type If this sample represents wastewater from a single institution, facility, or building, specify the institution type; otherwise, specify "not institution specific" Yes
epaid NPDES permit number for the wastewater treatment plant specified in 'wwtp_name' No
wwtp_name The name of the Wastewater Treatment Plant (WWTP) to which this wastewater flows. If this wastewater does not flow to a WWTP, specify an identifiable name for the septic or other treatment system to which this wastewater flows. An arbitrary name may be used if you do not wish to disclose the real name. Yes
wwtp_jurisdiction State, DC, US territory, or Freely Associated State jurisdiction name (2-letter abbreviation) in which the wastewater treatment plant provided in 'wwtp_name' is located Yes
capacity_mgd Wastewater treatment plant design capacity. This should be the capacity for which the plant is permitted. Yes
industrial_input Approximate average percentage of wastewater from industrial sources that is received by the wastewater treatment plant specified in 'wwtp_name' No
stormwater_input Does the wastewater treatment plant specified in 'wwtp_name' treat water from a combined sewer system (i.e., a sewer system that collects both sewage and stormwater)? No
influent_equilibrated Is influent to the wastewater treatment plant specified in 'wwtp_name' ever stored prior to treatment to equilibrate or modulate the influent flow rate? No
sample_type Type of sample collected, whether grab or composite. If composite, also provide the duration of sampling and type of composite, as listed in the Value Set (e.g., "24-hr flow-weighted composite"). A grab sample is defined as an individual sample collected without compositing or adding other samples, regardless of whether the sample matrix is liquid wastewater or sludge. Yes
composite_freq Frequency of sub-sample collection (for composite samples only): for flow-weighted, the number of sub-samples collected per million gallons of flow; for time-weighted, the number of sub-samples per hour. Flow-weighted example: a value of 5 would indicate 5 sub-samples per million gallons, or 1 sub-sample per 200,000 gallons No
sample_matrix Wastewater matrix from which the sample was collected Yes
collection_storage_time Duration of time the sample was stored after collection and prior to reaching the lab No
collection_storage_temp Temperature at which the sample was stored after collection and prior to reaching the lab No
pretreatment Was the sample treated with any chemicals prior to reaching the lab? These could include chemicals, such as stabilizers, added to the sample or chemicals, such as chlorine, added to the wastewater treatment train upstream of the sample collection point. Pasteurization should be specified in the 'pasteurized' field. No if 'pretreatment' is "yes", then specify in 'pretreatment_specify'
pretreatment_specify If 'pretreatment' is "yes", then specify the chemicals used No if 'pretreatment' is "yes", then specify in 'pretreatment_specify'
solids_separation Process used to separate solid and liquid phases of the sample, either prior to or in the absence of the concentration method specified in 'concentration_method' No
concentration_method Method used to concentrate the sample prior to analysis of the concentrate Yes
extraction_method Method used for nucleic acid extraction from the sample Yes
pre_conc_storage_time The approximate average duration of time between when samples reach the lab and when they are concentrated (if concentrated) No
pre_conc_storage_temp The storage temperature of samples after reaching the lab and prior to concentration (if concentrated) No
pre_ext_storage_time The approximate average duration of time between when samples are concentrated (if concentrated) and when they are extracted No
pre_ext_storage_temp The storage temperature of samples after concentration (if concentrated) and prior to extraction No
tot_conc_vol Total volume of sample concentrated (if concentrated); this total volume is not necessarily assayed and is not necessarily equal to the value specified in 'equiv_sewage_amt' No
ext_blank Are extraction blanks included in the extraction process? No
rec_eff_target_name Name of the recovery efficiency control target that is spiked in Yes If 'rec_eff_percent' is equal to a value other than "-1", then this must have a non-empty value
rec_eff_spike_matrix Matrix into which the recovery efficiency control target is spiked Yes If 'rec_eff_target_name' has a non-empty value, then this must have a non-empty value
rec_eff_spike_conc Spike concentration, on average, of the recovery control on a per sample volume basis Yes If 'rec_eff_target_name' has a non-empty value, then this must have a non-empty value
pasteurized Was the sample pasteurized? No
pcr_target The target of the PCR quantification Yes
pcr_gene_target The PCR gene used to quantify PCR target Yes The value selected for pcr_gene_target should align with the value selected for pcr_target
pcr_gene_target_ref A publication, website, or brief description of the PCR gene target used Yes
pcr_type The type of PCR used to quantify the PCR target Yes
lod_ref A publication, website, or brief description of the method used to calculate the limit of detection Yes
hum_frac_target_mic Name of microbial target used to estimate human fecal content No If 'hum_frac_mic_conc' has a non-empty value, then this must have a non-empty value
hum_frac_target_mic_ref A publication, website, or brief description of the microbial target specified in 'hum_frac_target_mic' No If 'hum_frac_mic_conc' has a non-empty value, then this must have a non-empty value
hum_frac_target_chem Name of chemical compound used to estimate human fecal content No If 'hum_frac_chem_conc' has a non-empty value, then this must have a non-empty value
hum_frac_target_chem_ref A publication, website, or brief description of the chemical compound specified in 'hum_frac_target_chem' No If 'hum_frac_chem_conc' has a non-empty value, then this must have a non-empty value
other_norm_name Name of a target or compound not specified in 'hum_frac_target_mic' or 'hum_frac_target_chem' used to estimate human fecal content No If 'other_norm_conc' has a non-empty value, then this must have a non-empty value
other_norm_ref A publication, website, or brief description of the target or compound specified in 'other_norm_name' No If 'other_norm_conc' has a non-empty value, then this must have a non-empty value
quant_stan_type The type of nucleic acid used as a standard for the PCR target quantification Yes
stan_ref A publication, website, or brief description of the quantitative standard material used Yes
inhibition_method A publication, website, or brief description of the method used to evaluate molecular inhibition Yes
num_no_target_control Number of no-template controls (NTC) per instrument run Yes
sample_collect_date The date of sample collection; for composite samples, specify the date on which sample collection began Yes
sample_collect_time The local time of sample collection; for composite samples, specify the time at which sample collection began Yes
time_zone Current local time zone corresponding to the time specified in 'sample_collect_time', represented as a UTC time offset (e.g., UTC-06:00) No
flow_rate Wastewater volumetric flow rate at the sample collection location over the 24-hr period during which the sample was collected. If only an instantaneous flow measurement is available, it may be reported in units of million gallons per day. Yes If 'sars_cov2_units' is on a per volume wastewater basis (e.g., copies/l wastewater), then this must have a non-empty value (i.e., measurements of wastewater solids are permitted empty values for 'flow_rate')
ph pH of wastewater sample (if sludge, pH of influent at time of collection) No
conductivity Specific conductivity of wastewater sample (if sludge, conductivity of influent at time of collection) No
tss Total suspended solids of raw (or, if unavailable, post-grit removal) wastewater No
collection_water_temp Sample temperature at time of collection No
equiv_sewage_amt Equivalent unconcentrated volume of wastewater or mass of sludge in PCR reaction No
sample_id An ID assigned to a wastewater sample. It must be unique for this NWSS reporting jurisdiction. Wastewater samples that are split and measured by different labs should have the same sample ID but different lab IDs. Wastewater samples for which multiple PCR targets are measured should also have the same sample ID. Note: do not include PII in this field. Yes
lab_id An ID assigned to a testing lab. It must be unique across labs used for this NWSS reporting jurisdiction's testing. If the same lab is used across multiple NWSS reporting jurisdictions, each NWSS reporting jurisdiction may assign that lab a different lab ID. Note: including PII in this field is discouraged. Yes
qc_ignore Should the DCIPHER QC report ignore this sample? The purpose of this field is to allow for removal of samples from the QC report so that samples with unresolvable QC issues do not clutter the report. This field only affects whether samples are shown in the QC report, not how they are otherwise processed or analyzed. If the value is "yes", then this sample will be excluded from the QC report. If the value is "no" or [empty], then this sample will be included in the QC report for up to 90 days. Samples will only be included in the QC report for up to 90 days, regardless of the value specified in ‘qc_ignore’. No
dashboard_ignore Should the DCIPHER dashboard ignore this sample? If the value is "yes", then this sample will be excluded from the dashboard. If the value is "no" or [empty], then this sample will be included in the dashboard.
In addition to excluding the sample from the DCIPHER dashboard, this field will also prevent the sample from public data release.
No
analysis_ignore Should DCIPHER data analysis ignore this sample? If the value is "yes", then this sample will be excluded from DCIPHER data analysis, which will also cause it to be excluded from the dashboard. If the value is "no" or [empty], then this sample will be included in DCIPHER data analysis. No
test_result_date The date on which this PCR target measurement was made Yes
pcr_target_units Units of PCR target sample concentration Yes The units should relate to the PCR target indicated in pcr_target
pcr_target_avg_conc Concentration of the PCR target back-calculated to unconcentrated sample basis; enter "0" if no amplification occurred, using the definition of amplification described in 'ntc_amplify'; otherwise, enter the estimated concentration; do not adjust for matrix recovery efficiency Yes The concentration should relate to the PCR target indicated in pcr_target
pcr_target_std_error Standard error (SE) of the PCR target in wastewater sample, or best estimate that is consistently available. If sample replicates are always performed, use SE of sample replicates; else, if processing replicates are always performed, use SE of processing replicates; else, if qPCR is performed, use SE of PCR replicates; else, if digital PCR is performed, use error from multiple replicates if available, and Poisson error if not No The standard error should relate to the PCR target indicated in pcr_target
pcr_target_cl_95_lo Lower bound of 95% confidence interval of the PCR target in wastewater sample, or best estimate that is consistently available. Follow the same hierarchy as described for standard error. (Note: 'cl' stands for confidence limit) No The lower bound of the 95% confidence interval should relate to the PCR target indicated in pcr_target
pcr_target_cl_95_up Upper bound of 95% confidence interval of the PCR target in wastewater sample, or best estimate that is consistently available. Follow the same hierarchy as described for standard error. (Note: 'cl' stands for confidence limit) No The upper bound of the 95% confidence interval should relate to the PCR target indicated in pcr_target
pcr_target_below_lod Was the concentration of the PCR target below the limit of detection? No Whether the concentration was below the LOD should be based on the LOD of the PCR target indicated in lod_sewage
lod_sewage PCR target limit of detection back-calculated to unconcentrated sample basis Yes The LOD should relate to the PCR target indicated in pcr_target
ntc_amplify For qPCR, did any no-template controls on this instrument run have a Ct value less than 40? For ddPCR, did any no-template controls on this instrument run have 3 or more positive droplets? Yes
rec_eff_percent Percent of spiked recovery control, specified in 'rec_eff_target_name', that was recovered Yes
inhibition_detect Was molecular inhibition detected? Yes
inhibition_adjust Was inhibition incorporated into the PCR target concentration calculation? Yes If 'inhibition_detect' is "yes", then this must have a non-empty value
hum_frac_mic_conc Concentration of microbial target specified in 'hum_frac_target_mic'; follow the same guidelines outlined for 'pcr_target_avg_conc' No If 'hum_frac_mic_unit' has a non-empty value, then this must have a non-empty value
hum_frac_mic_unit Concentration units of microbial target specified in 'hum_frac_target_mic' No If 'hum_frac_mic_conc' has a non-empty value, then this must have a non-empty value
hum_frac_chem_conc Concentration of chemical target specified in 'hum_frac_target_chem' No If 'hum_frac_chem_unit' has a non-empty value, then this must have a non-empty value
hum_frac_chem_unit Concentration units of chemical target specified in 'hum_frac_target_chem' No If 'hum_frac_chem_conc' has a non-empty value, then this must have a non-empty value
other_norm_conc Concentration of target spcified in 'other_norm_name' No If 'other_norm_unit' has a non-empty value, then this must have a non-empty value
other_norm_unit Concentration units of target spcified in 'other_norm_name' No If 'other_norm_conc' has a non-empty value, then this must have a non-empty value
quality_flag Does this observation have quality control issues? No
major_lab_method A number used to distinguish major lab methods at the reporting jurisdiction level. Differences in lab methods may limit the comparability of PCR target concentrations. Reporting jurisdictions should use this field to indicate that lab methods are sufficiently different that caution should be used when comparing PCR target concentrations across them. These differences may result from any lab processing or quantification steps, including different PCR gene targets. Note that 'major_lab_method' may be the same for different laboratories if the reporting jurisdiction believes concentrations measured by those laboratories are comparable; conversely, 'major_lab_method' may be different for the same laboratory if changes made to the laboratory protocol rendered concentrations not comparable. No
major_lab_method_desc Brief description of the basis for assigning a ‘major_lab_method’, e.g., "this lab uses a lab method distinct from other labs in this reporting jurisdiction", "this lab changed lab methods - new concentration method" or "lab method is same as other labs in this reporting jurisdiction but performance differs" No

Sheet 4: NWSS wastewater data valuesets

Field Value Set Name

Value Set Name:
vs_concentration_method

vs_extraction_method

vs_hum_frac_target_chem

vs_hum_frac_target_mic

vs_institution_type

vs_mic_chem_units

vs_mic_chem_units_e

vs_num_no_target_control

vs_other_norm_name

vs_pcr_target

vs_pcr_gene_target

vs_pcr_type

vs_quant_stan_type

vs_rec_eff_spike_matrix

vs_rec_eff_target_name

vs_reporting_jurisdiction

vs_sample_location

vs_sample_matrix

vs_sample_type

vs_solids_separation

vs_wwtp_jurisdiction

vs_yn

vs_yne

vs_ynn






Value Set Description
Value Set Description
Value Set Description
Value Set Description
Value Set Description
Value Set Description
Value Set Description
Value Set Description
Value Set Description
Value Set Description
Value Set Description
Value Set Description
Value Set Description
Value Set Description
Value Set Description
Value Set Description
Value Set Description
Value Set Description
Value Set Description
Value Set Description
Value Set Description
Value Set Description
Value Set Description
Value Set Description
concentration_method vs_concentration_method



membrane filtration with addition of mgcl2

qiagen allprep powerviral dna/rna kit

caffeine

pepper mild mottle virus

not institution specific This sample does not represent wastewater from a single institution, facility, or building
copies/L wastewater

copies/L wastewater

0
pepper mild mottle virus
sars-cov-2

n1 sars-cov-2 gene target
qpcr Real-time PCR, also called 'quantitative' PCR
dna

raw sample

bcov vaccine
AL Alabama
wwtp A sampling location at a wastewater treatment plant or other community-scale treatment infrastructure specified in 'wwtp_name'
raw wastewater Wastewater without any form of treatment applied to it
grab An individual sample collected without compositing or adding other samples
filtration Filtration to remove solids from a wastewater sample prior to further concentration or analysis of the liquid filtrate
AL Alabama
yes

yes

yes
extraction_method vs_extraction_method



membrane filtration with sample acidification

qiagen allprep powerfecal dna/rna kit

creatinine

crassphage

correctional A prison, penitentiary, penal facility, jail, detention unit, or other facility in which persons are incarcerated by government officials
log10 copies/L wastewater
log10 copies/L wastewater
1
crassphage
delta

n2 sars-cov-2 gene target
ddpcr Used to refer specifically to BioRad digital droplet emulsification technology
rna

raw sample post pasteurization

bcov culture
AK Alaska
upstream A sampling location other than "wwtp"
post grit removal Wastewater after removal of large solids at a treatment plant but prior to a primary clarifier
48-hr flow-weighted composite

centrifugation Centrifugation prior to or in the absence of a concentration step; centrifugation as part of a flocculation or precipitation process, or part of a filtration-based concentration process, is considered part of a concentration method; centrifugation to dewater sludge samples is considered a solids separation process, not a concentration process
AK Alaska
no

no

no
hum_frac_target_chem vs_hum_frac_target_chem



membrane filtration with acidification and mgcl2

qiagen allprep dna/rna kit

sucralose

hf183

long term care - nursing home A residential healthcare facility that provides 24-medical care. These are also called skilled nursing facilities. Generally licensed by CMS.
copies/g wet sludge Wet sludge mass is based on the mass of sludge without drying the solids in a drying oven
copies/g wet sludge Wet sludge mass is based on the mass of sludge without drying the solids in a drying oven
2
hf183

omicron

n3 sars-cov-2 gene target
qiagen dpcr




clarified sample

brsv vaccine
AS American Samoa



primary sludge Sludge from the primary clarifier
47-hr flow-weighted composite

gravity settling Solids separated from liquid by allowing solids to settle by gravity
AS American Samoa



[empty]

not tested
hum_frac_target_mic vs_hum_frac_target_mic



membrane filtration with no amendment

qiagen rneasy powermicrobiome kit

ibuprofen

f+ rna coliphage

long term care - assisted living A residential facility that provides assistance with daily care but generally does not provide skilled nursing care. May be licensed as healthcare facility based on state regulations.
log10 copies/g wet sludge Wet sludge mass is based on the mass of sludge without drying the solids in a drying oven
log10 copies/g wet sludge Wet sludge mass is based on the mass of sludge without drying the solids in a drying oven
3
f+ rna coliphage



e_sarbeco sars-cov-2 gene target
fluidigm dpcr




sample concentrate
brsv culture
AZ Arizona



primary effluent Effluent from the primary clarifier
46-hr flow-weighted composite

none

AZ Arizona








institution_type vs_institution_type



membrane filtration with addition of mgcl2, membrane recombined with separated solids

qiagen powerwater kit

[empty]

f+ dna coliphage

other long term care Other residential facilities that provide daily and/or medical care, but are not defined as nursing home/skilled nursing facilities or assisted living facilities. An example of this is a state-licensed intermediate care facility.
copies/g dry sludge Dry sludge mass is based on the mass of solids after drying in a drying oven
copies/g dry sludge Dry sludge mass is based on the mass of solids after drying in a drying oven
more than 3

f+ dna coliphage



n_sarbeco sars-cov-2 gene target
life technologies dpcr




lysis buffer

murine coronavirus

AR Arkansas



secondary sludge Sludge from the secondary clarifier
45-hr flow-weighted composite

[empty]

AR Arkansas








sars_cov2_units vs_mic_chem_units



membrane filtration with sample acidification, membrane recombined with separated solids

qiagen rneasy kit




[empty]

short stay acute care hospital

log10 copies/g dry sludge Dry sludge mass is based on the mass of solids after drying in a drying oven
log10 copies/g dry sludge Dry sludge mass is based on the mass of solids after drying in a drying oven



caffeine



rdrp_sarsr sars-cov-2 gene target
raindance dpcr




dewatered solids

oc43

CA California



secondary effluent Efluent from the secondary clarifier
44-hr flow-weighted composite




CA California








hum_frac_chem_unit vs_mic_chem_units_e



membrane filtration with acidification and mgcl2, membrane recombined with separated solids

qiagen ez1 virus mini kit v2.0







long term acute care hospital Acute care hospitals that provide care for patients with average length of stay longer than 25 days. LTACH patients are often transferred from intensive care units of ACH.
micrograms/L wastewater
micrograms/L wastewater




creatinine




niid_2019-ncov_n sars-cov-2 gene target






[empty]

phi6

CI Chicago, IL



septage Wastewater sampled from within a septic tank
43-hr flow-weighted composite




CO Colorado








other_norm_units vs_mic_chem_units_e



membrane filtration with no amendment, membrane recombined with separated solids

promega ht tna kit







child day care

log10 micrograms/L wastewater

log10 micrograms/L wastewater




sucralose




rdrp gene / ncov_ip2 sars-cov-2 gene target









puro

CO Colorado



holding tank Wastewater sampled from a holding tank, such as from an airplane or ship
42-hr flow-weighted composite




MP Commonwealth of Northern Mariana Islands








hum_frac_mic_unit vs_mic_chem_units_e



peg precipitation

promega automated tna kit







k12 A school serving students in the kindergarten to 12th grade range
micrograms/g wet sludge Wet sludge mass is based on the mass of sludge without drying the solids in a drying oven
micrograms/g wet sludge Wet sludge mass is based on the mass of sludge without drying the solids in a drying oven



ibuprofen




rdrp gene / ncov_ip4 sars-cov-2 gene target









ms2 coliphage

MP Commonwealth of Northern Mariana Islands






41-hr flow-weighted composite




CT Connecticut








num_no_target_control vs_num_no_target_control



aloh3 precipitation

promega manual tna kit







higher ed dorm Solely higher education dormitory buildings
log10 micrograms/g wet sludge Wet sludge mass is based on the mass of sludge without drying the solids in a drying oven
log10 micrograms/g wet sludge Wet sludge mass is based on the mass of sludge without drying the solids in a drying oven



rnase p




taqpath n sars-cov-2 gene target









hep g armored rna

CT Connecticut






40-hr flow-weighted composite




DE Delaware








other_norm_name vs_other_norm_name



ultracentrifugation

promega wastewater large volume tna capture kit







higher ed other Higher education buildings or facilities that do not include dorms or that include dorms and other buildings
micrograms/g dry sludge Dry sludge mass is based on the mass of solids after drying in a drying oven
micrograms/g dry sludge Dry sludge mass is based on the mass of solids after drying in a drying oven



[empty]




taqpath s sars-cov-2 gene target









heat inactivated sars-cov-2 virus

DE Delaware






39-hr flow-weighted composite




DC District of Columbia








pcr_target vs_pcr_target



skimmed milk flocculation

nuclisens automated magnetic bead extraction kit







social services shelter Other type of social services shelter
log10 micrograms/g dry sludge Dry sludge mass is based on the mass of solids after drying in a drying oven
log10 micrograms/g dry sludge Dry sludge mass is based on the mass of solids after drying in a drying oven









orf1b sars-cov-2 gene target









[empty]

DC District of Columbia






38-hr flow-weighted composite




FM Federated States of Micronesia








pcr_gene_target vs_pcr_gene_target



beef extract flocculation

nuclisens manual magnetic bead extraction kit







other residential building Individual residential buildings or institutions not captured in other categories



[empty]










orf1ab sars-cov-2 gene target












FM Federated States of Micronesia






37-hr flow-weighted composite




FL Florida








pcr_type vs_pcr_type



promega wastewater large volume tna capture kit

phenol chloroform







ship
















n1 and n2 combined sars-cov-2 gene target; Detection of N1 and N2 targets was performed on a single fluorescence channel, such that distinct measurements for N1 and N2 do not exist












FL Florida






36-hr flow-weighted composite




GA Georgia








quant_stan_type vs_quant_stan_type



centricon ultrafiltration

chemagic viral dna/rna 300 kit







airplane
















n sars-cov-2 gene target












GA Georgia






35-hr flow-weighted composite




GU Guam








rec_eff_spike_matrix vs_rec_eff_spike_matrix



amicon ultrafiltration

trizol, zymo mag beads w/ zymo clean and concentrator







other worksite Any commercial facility not captured in other commercial facility categories















s sars-cov-2 gene target












GU Guam






34-hr flow-weighted composite




HI Hawaii








rec_eff_target_name vs_rec_eff_target_name



hollow fiber dead end ultrafiltration

4s method (https://www.protocols.io/view/v-4-direct-wastewater-rna-capture-and-purification-bpdfmi3n)

























orf1a sars-cov-2 gene target












HI Hawaii






33-hr flow-weighted composite




ID Idaho








reporting_jurisdiction vs_reporting_jurisdiction



innovaprep ultrafiltration

qiagen qiaamp buffers with epoch columns

























ddcov_n  sars-cov-2 gene target












HO Houston, TX






32-hr flow-weighted composite




IL Illinois








sample_location vs_sample_location



no liquid concentration, liquid recombined with separated solids

zymo quick-rna fungal/bacterial miniprep #r2014

























ddcov_e sars-cov-2 gene target












ID Idaho






31-hr flow-weighted composite




IN Indiana








sample_matrix vs_sample_matrix



ceres nanotrap

thermo magmax microbiome ultra nucleic acid isolation kit
























ip2 and ip4 combined sars-cov-2 gene target












IL Illinois






30-hr flow-weighted composite




IA Iowa








sample_type vs_sample_type



zymo environ water rna kit/ zymo environ water rna kit (cat. r2042)

zymo environ water rna kit/ zymo environ water rna kit (cat. r2042)
























a1306s delta gene target












IN Indiana






29-hr flow-weighted composite




KS Kansas








solids_separation vs_solids_separation



none




























p2046l delta gene target












IA Iowa






28-hr flow-weighted composite




KY Kentucky








wwtp_jurisdiction vs_wwtp_jurisdiction

































p2287s delta gene target












KS Kansas






27-hr flow-weighted composite




LA Louisiana








sars_cov2_below_lod vs_yn

































t3646a delta gene target












KY Kentucky






26-hr flow-weighted composite




ME Maine








ntc_amplify vs_yn

































v2930l delta gene target












LC Los Angeles County, CA






25-hr flow-weighted composite




MD Maryland








stormwater_input vs_yne

































g662s delta gene target












LA Louisiana






24-hr flow-weighted composite




MA Massachusetts








influent_equilibrated vs_yne

































p100l delta gene target












ME Maine






23-hr flow-weighted composite




MI Michigan








pretreatment vs_yne

































a1918v delta gene target












MD Maryland






22-hr flow-weighted composite




MN Minnesota








ext_blank vs_yne

































t19r delta gene target












MA Massachusetts






21-hr flow-weighted composite




MS Mississippi








pasteurized vs_yne

































e156g delta gene target












MI Michigan






20-hr flow-weighted composite




MO Missouri








inhibition_adjust vs_yne

































del 157/158 delta gene target












MN Minnesota






19-hr flow-weighted composite




MT Montana








quality_flag vs_yne

































l452r delta gene target












MS Mississippi






18-hr flow-weighted composite




NE Nebraska








inhibition_detect vs_ynn

































p681r delta gene target












MO Missouri






17-hr flow-weighted composite




NV Nevada












































d950n delta gene target












MT Montana






16-hr flow-weighted composite




NH New Hampshire












































i82t delta gene target












NE Nebraska






15-hr flow-weighted composite




NJ New Jersey












































d63g delta gene target












NV Nevada






14-hr flow-weighted composite




NM New Mexico












































r203m delta gene target












NH New Hampshire






13-hr flow-weighted composite




NY New York












































g215c delta gene target












NJ New Jersey






12-hr flow-weighted composite




NC North Carolina











































d377y delta gene target












NM New Mexico






11-hr flow-weighted composite




ND North Dakota











































k856r  omicron gene target












NY New York






10-hr flow-weighted composite




OH Ohio












































s2083i  omicron gene target












NZ New York City, NY






9-hr flow-weighted composite




OK Oklahoma












































del2084/2084  omicron gene target












NC North Carolina






8-hr flow-weighted composite




OR Oregon












































a2710t  omicron gene target












ND North Dakota






7-hr flow-weighted composite




PA Pennsylvania












































p3395h  omicron gene target












OH Ohio






6-hr flow-weighted composite




PR Puerto Rico












































del3674/3676  omicron gene target












OK Oklahoma






5-hr flow-weighted composite




MH Republic of the Marshall Islands












































i3758v  omicron gene target












OR Oregon






4-hr flow-weighted composite




PW Republic of Palau












































i1566v  omicron gene target












PA Pennsylvania






3-hr flow-weighted composite




RI Rhode Island












































a67v  omicron gene target












PH Philadelphia, PA






2-hr flow-weighted composite




SC South Carolina












































del69/70  omicron gene target












PR Puerto Rico






1-hr flow-weighted composite




SD South Dakota












































del143/145  omicron gene target












MH Republic of the Marshall Islands






48-hr time-weighted composite




TN Tennessee












































n211i  omicron gene target












PW Republic of Palau






47-hr time-weighted composite




TX Texas












































del212/212  omicron gene target












RI Rhode Island






46-hr time-weighted composite




VI U.S. Virgin Islands












































g339d  omicron gene target












SC South Carolina






45-hr time-weighted composite




UT Utah












































s371l  omicron gene target












SD South Dakota






44-hr time-weighted composite




VT Vermont












































s373p  omicron gene target












TN Tennessee






43-hr time-weighted composite




VA Virginia












































s375f  omicron gene target












TX Texas






42-hr time-weighted composite




WA Washington












































s477n  omicron gene target












VI U.S. Virgin Islands






41-hr time-weighted composite




WV West Virginia












































e484a  omicron gene target












UT Utah






40-hr time-weighted composite




WI Wisconsin












































q493r  omicron gene target












VT Vermont






39-hr time-weighted composite




WY Wyoming












































g496s  omicron gene target












VA Virginia






38-hr time-weighted composite



















































q498r  omicron gene target












WA Washington






37-hr time-weighted composite



















































y505h  omicron gene target












WV West Virginia






36-hr time-weighted composite



















































t547k  omicron gene target












WI Wisconsin






35-hr time-weighted composite



















































n679k  omicron gene target












WY Wyoming






34-hr time-weighted composite



















































d796y  omicron gene target





















33-hr time-weighted composite



















































n856k  omicron gene target





















32-hr time-weighted composite



















































q954h  omicron gene target





















31-hr time-weighted composite



















































n969k  omicron gene target





















30-hr time-weighted composite



















































l981f  omicron gene target





















29-hr time-weighted composite



















































t9i  omicron gene target





















28-hr time-weighted composite



















































d3g  omicron gene target





















27-hr time-weighted composite



















































q19e  omicron gene target





















26-hr time-weighted composite



















































a63t  omicron gene target





















25-hr time-weighted composite











































































24-hr time-weighted composite











































































23-hr time-weighted composite











































































22-hr time-weighted composite











































































21-hr time-weighted composite











































































20-hr time-weighted composite











































































19-hr time-weighted composite











































































18-hr time-weighted composite











































































17-hr time-weighted composite











































































16-hr time-weighted composite











































































15-hr time-weighted composite











































































14-hr time-weighted composite











































































13-hr time-weighted composite











































































12-hr time-weighted composite











































































11-hr time-weighted composite











































































10-hr time-weighted composite











































































9-hr time-weighted composite











































































8-hr time-weighted composite











































































7-hr time-weighted composite











































































6-hr time-weighted composite











































































5-hr time-weighted composite











































































4-hr time-weighted composite











































































3-hr time-weighted composite











































































2-hr time-weighted composite











































































1-hr time-weighted composite











































































48-hr manual composite











































































47-hr manual composite











































































46-hr manual composite











































































45-hr manual composite











































































44-hr manual composite











































































43-hr manual composite











































































42-hr manual composite











































































41-hr manual composite











































































40-hr manual composite











































































39-hr manual composite











































































38-hr manual composite











































































37-hr manual composite











































































36-hr manual composite











































































35-hr manual composite











































































34-hr manual composite











































































33-hr manual composite











































































32-hr manual composite











































































31-hr manual composite











































































30-hr manual composite











































































29-hr manual composite











































































28-hr manual composite











































































27-hr manual composite











































































26-hr manual composite











































































25-hr manual composite











































































24-hr manual composite











































































23-hr manual composite











































































22-hr manual composite











































































21-hr manual composite











































































20-hr manual composite











































































19-hr manual composite











































































18-hr manual composite











































































17-hr manual composite











































































16-hr manual composite











































































15-hr manual composite











































































14-hr manual composite











































































13-hr manual composite











































































12-hr manual composite











































































11-hr manual composite











































































10-hr manual composite











































































9-hr manual composite











































































8-hr manual composite











































































7-hr manual composite











































































6-hr manual composite











































































5-hr manual composite











































































4-hr manual composite











































































3-hr manual composite











































































2-hr manual composite











































































1-hr manual composite















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