Download:
pdf |
pdfLab ID No. [][][][][][]
2025 LABORATORY TESTING PRACTICES FOR CANDIDEMIA QUESTIONNAIRE
1) What kind of laboratory is this? (select one)
Hospital laboratory
Commercial laboratory (Quest, etc.)
Other (specify) ______________________
Unknown
2) Does this laboratory ever receive blood cultures from nursing homes or other long term care facilities?
Yes
No
Unknown
3) Does the clinical microbiology laboratory at your institution have a separate mycology section or laboratory?
Yes
No
Unknown
4) What kind of blood culture system does your laboratory use? (check all that apply)
BacT/Alert
Bactec 9240
BacT/Alert 3D
Bactec FX
VersaTREK
Other (specify) ______________________
5) What is the approximate volume of fungal cultures ordered and performed annually in your laboratory for
any specimen type?
Specify number: ______________
Unknown
6) What is the approximate volume of fungal blood cultures ordered and performed annually in your laboratory?
Specify number: ______________ Unknown
YEAST IDENTIFICATION
7) Does this laboratory offer yeast identification (either onsite or sent to another laboratory)?
Yes
No (-------- If No, SKIP TO QUESTION 15 --------)
Unknown (is there another laboratory staff member who can assist with the questionnaire?)
8) Where is yeast identification done? (check the most applicable)
On-site, in the laboratory
Sent to commercial lab
Sent to affiliated hospital lab
Sent to other local/regional, non-affiliated reference or public health laboratory
Other ______________________________
Unknown
Answer the following questions for the lab selected in question 8.
9) How does this lab identify yeast? (check all that apply)
MALDI-TOF Bruker (Biotyper)
MALDI-TOF bioMerieux (VITEK MS)
VITEK 2
API 20C
DNA sequencing
PNA-FISH
BactiCard Candida
BD Phoenix
MicroScan
RapID Plus
Other (specify) ______________________
Unknown
10) Does this laboratory routinely use chromogenic agar for the identification or differentiation of Candida
isolates?
Yes
No
Unknown
11) Species-level identification is performed for Candida spp. isolated from which of the following?
a. Blood isolates
Yes, always
2025
Yes, with clinician order
No
Unknown
Page 1 of 4
Lab ID No. [][][][][][]
b. Other normally sterile body site isolates
Yes, always
Yes, with clinician order
c. Abdominal isolates
Yes, always
Yes, with clinician order
No
Unknown
No
Unknown
d. Respiratory isolates
Yes, always
Yes, with clinician order
No
Unknown
e. Urine isolates
Yes, always
Yes, with clinician order
No
Unknown
Other (specify) ____________________
Yes, always
Yes, with clinician order
No
Unknown
f.
12) How does this laboratory meet proficiency testing requirements for yeast identification?
Commercial provider (specify) _________________
Internal alternate assessments (specify) __________________
13) Does this laboratory employ molecular tests to identify Candida from blood specimens?
Yes (go to Q13a)
No (go to Q14)
Unknown
a. If Yes, check all molecular tests that apply and indicate when this laboratory first started using that
molecular test.
Molecular Test
T2Candida Panel
BioFire (FilmArray)
GenMark
Other (specify): ____________________
Date Lab Started using Test (mm/dd/yyyy):
____/____/________
Unknown
____/____/________
Unknown
____/____/________
Unknown
____/____/________
Unknown
b. If Yes to any of the above molecular tests and a positive result is observed, does this laboratory
culture the blood to obtain an isolate?
Yes, always
No
Yes, with a clinical order
Unknown
14) If No for Question 13, does this laboratory have plans to employ molecular tests for Candida identification in
the near future (e.g., T2Candida Panel, BioFire, GenMark)?
Yes (specify) ___________________
Unknown
No
Not applicable
ANTIFUNGAL SUSCEPTIBILITY TESTING
15) Does this laboratory offer any antifungal susceptibility testing (AFST) for Candida (either onsite or sent to
another laboratory)?
Yes
No (-------- If No, QUESTIONNAIRE COMPLETE --------)
Unknown (is there another laboratory staff member who can assist with the questionnaire?)
16) Where is AFST done? (check the most applicable)
On-site, in the laboratory (go to Q17)
Sent to commercial lab (-------- If not an on-site laboratory, QUESTIONNAIRE COMPLETE --------)
Sent to affiliated hospital lab
Sent to other local/regional, non-affiliated reference or public health laboratory
Other ______________________________
Unknown
2025
Page 2 of 4
Lab ID No. [][][][][][]
Answer the following questions for the lab selected in question 16.
17) Is AFST available for any of the following antifungal drugs (check all that apply):
Fluconazole
Caspofungin
Voriconazole
Amphotericin B
Itraconazole
Flucytosine
Posaconazole
Rezafungin
Micafungin
Other (specify) __________________
Anidulafungin
Unknown
18) What methods are used for AFST, excluding Amphotericin B? (check all that apply)
Broth microdilution with laboratory
Vitek2 (bioMerieux)
developed plates
Disk diffusion
YeastOne (Thermo Scientific™ Sensititre™)
Other ______________________________
Gradient diffusion (E test)
Unknown
19) What methods are used for AFST of Amphotericin B? (check all that apply)
Broth microdilution with
Vitek2 (bioMerieux)
laboratory developed plates
Other ______________________________
YeastOne (Thermo Scientific™ Sensititre™)
Unknown
Gradient diffusion (E test)
20) How does this laboratory meet proficiency testing requirements for AFST, if performed?
Commercial provider (specify) _________________
Internal alternate assessments (specify) __________________
21) How are results of AFST reported when breakpoints are available? (select one)
Categorical interpretation only (susceptible,
Both--categorical interpretation PLUS MIC
resistant, etc.)
Unknown
MIC only
22) How are results of AFST reported when breakpoints aren’t available? (select one)
MIC only
Epidemiological cutoff values (ECVs)
Unknown
23) For what type of Candida isolates is AFST performed automatically? (check all that apply)
Blood isolates
No AFST performed automatically (requires
Other normally sterile body site isolates
order from a clinician)
Other (specify) ______________________
Unknown
24) When is AFST performed for the following Candida spp.?
a. C. albicans
Performed automatically (Go to 24ai)
Performed with a clinician’s order (Go to 24ai)
Not performed
i.
2025
Drugs for which AFST is performed on C. albicans (check all that apply):
Micafungin
Fluconazole
Other
Anidulafungin
Voriconazole
Unknown
Caspofungin
Amphotericin B
Page 3 of 4
Lab ID No. [][][][][][]
b. C. glabrata
Performed automatically (Go to 24bi)
Performed with a clinician’s order (Go to 24bi)
Not performed
i.
Drugs for which AFST is performed on C. glabrata (check all that apply):
Micafungin
Fluconazole
Other
Anidulafungin
Voriconazole
Unknown
Caspofungin
Amphotericin B
c. C. parapsilosis
Performed automatically (Go to 24ci)
Performed with a clinician’s order (Go to 24ci)
Not performed
i.
Drugs for which AFST is performed on C. parapsilosis (check all that apply):
Micafungin
Fluconazole
Other
Anidulafungin
Voriconazole
Unknown
Caspofungin
Amphotericin B
d. Other Candida spp.
Performed automatically (Go to 24di)
Performed with a clinician’s order (Go to 24di)
Not performed
i.
Drugs for which AFST is performed on other Candida spp. (check all that apply):
Micafungin
Fluconazole
Other
Anidulafungin
Voriconazole
Unknown
Caspofungin
Amphotericin B
25) Is this laboratory tracking susceptibility trends for Candida spp. isolates tested in your lab?
Yes
No
Unknown
------------------------------------------------ END OF QUESTIONNAIRE ------------------------------------------------
2025
Page 4 of 4
| File Type | application/pdf |
| Author | Angela Ahlquist |
| File Modified | 2024-08-24 |
| File Created | 2024-08-24 |