NPDES Permittee Data Report Form

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PRE-SORTED
FIRST-CLASS MAIL
POSTAGE & FEES PAID
EPA
PERMIT NO. G-35

United States
Environmental Protection Agency
OECA MC 2227A
WASHINGTON DC 20460
———————————————
Official Business

TIME SENSITIVE – RESPONSE NEEDED
Mailroom: Please forward to responsible office.

IMPORTANT NOTICE TO NPDES PERMITTEES
DMR-QA Study 45 Announcement
IMPORTANT DATES TO REMEMBER
June 6, 2025 ................ DMR-QA Study 45 begins.
July 14, 2025 ............... Submit address verification to State DMR-QA Coordinator.
August 29, 2025 .......... DMR-QA Study 45 ends.
October 24, 2025......... Submit DMR-QA 45 Results to State DMR-QA Coordinator.
December 5, 2025 ....... Submit corrective action reports and retest results to State DMR-QA
Coordinator, if applicable.

DMR-QA STUDY 45
Immediately verify receipt of DMR-QA Study 45 by either filling out the form below and mailing this page to your State DMR-QA
Coordinator (listed on pages 10-11) or follow the e-mail instructions at the bottom of this page.
The mailed form must be postmarked on or before July 14, 2025.
NPDES PERMITTEE ADDRESS VERIFICATION FORM
Discharge Monitoring Report - Quality Assurance (DMR-QA) Study 45
Please provide corrections to the mailing address where all DMR-QA study paperwork should be sent.
NPDES Permit Number
(2-character State Code + 7-digit Permit Code as shown on the mailing label—for example, AL1234567)
If contact/address information from the
mailing label or e-mail is correct, please check this box only → ❏
Facility Name
Contact Name

Title

Mailing Address
City

State

Phone Number

Fax Number

Zip Code

E-mail Address

ELECTRONIC NOTIFICATION PROCEDURE
You may verify receipt electronically by sending an e-mail on or before July 14, 2025 to your State DMR-QA Coordinator (listed
on pages 10-11 of the enclosed instructions). The e-mail should be composed in the following manner:
Subject line should contain ONLY the NPDES Permit number (2-character State Code + 7-digit Permit Code as shown on the
mailing label, for example AZ1234567).
If you require any changes to the mailing label on this announcement, the body of the e-mail should contain a list including:
Company name; Contact Name/Title; Mailing Address; City; State; Zip Code; and Facility Type (select one: federal, state, local
or commercial/private). Otherwise, you may simply write “No changes to address or contact information” in the body of the email.

Page 2

June 5, 2025

Dear NPDES Permit Holder:
This letter initiates the 2025 Discharge Monitoring Report - Quality Assurance (DMR-QA) Study 45. By receipt of this letter, you
are required under Clean Water Act (CWA) Section 308 to participate in DMR-QA Study 45 unless your facility is covered by an
EPA waiver.1 Your participation plays a key role in monitoring the quality of data used to assure the integrity of the CWA’s National
Pollutant Discharge Elimination System (NPDES) Program and assures your ability to maintain compliance with NPDES permit
requirements and conditions.
DMR-QA Study 45 covers major and select minor NPDES permit holders. You (the permittee) are responsible for ensuring that
results of DMR-QA Study 45 tests, performed by your in-house and/or contract laboratories, are graded by an accredited
Proficiency Testing (PT) Provider. If any graded test results are “Not Acceptable,” you must follow up with the laboratory to
determine the cause of the deficiency and ensure corrective action is taken to prevent future occurrences. While performing
tests and analyses, please ensure that your test methods/procedures follow 40 CFR part 136 regulations and applicable
guidance. Use the same personnel and equipment as you would for routine NPDES permit compliance monitoring tests.
Further Information
Permittees with valid e-mail addresses will receive the DMR-QA Study 45 package by e-mail. You may request a hard copy from
EPA by sending an e-mail to [email protected]. Questions on the national program should be addressed to Greg Savitske
([email protected], 202-564-2601), EPA’s National DMR-QA Coordinator. State and EPA Regional DMR-QA contact information
is provided on pages 9-11. Please reference your NPDES permit number on all correspondence.
Thank you for your attention to this CWA Section 308 requirement.
Sincerely,

Loren Denton, Director
Monitoring, Assistance, and Media Programs Division
Office of Compliance
Enclosures

1

EPA is authorized to collect this information under Section 308 of the CWA, 33 U.S.C. § 1318. This information request is
enforceable under Section 309 of the CWA, 33 U.S.C. § 1319. EPA may grant a waiver from participating in DMR-QA to states
with laboratory quality assurance programs approved by EPA as a substitute for the DMR-QA Study. Refer to footnotes 2, 3,
and 4 on page 5 to determine if you are covered by an EPA waiver or contact your State DMR-QA Coordinator.
Page 3

DMR-QA STUDY 45
Table of Contents
DMR-QA Study 45 Frequently Asked Questions ................ 5
NPDES Permittee Instructions .......................................... 6
Chemistry/Microbiology Laboratory Instructions ............... 7
WET Testing Laboratory Instructions ................................. 8
Accredited Proficiency Testing Providers .......................... 9
EPA Regional and State DMR-QA Coordinators ................. 9
NPDES Permittee Data Report Form ............................... 12
Chemistry/Microbiology Analyte Checklist ...................... 15
Whole Effluent Toxicity (WET) Analyte Checklist .............. 16

Schedule and Checklist for DMR-QA Study 45
Deadline*
June 6, 2025

July 14, 2025

Permittee
❏ Study 45 begins
❏ Notify all laboratories of DMR-QA Study 45

In-House and Contract Laboratories

PT Providers

❏ Study 45 begins
❏ Order test samples from PT Provider. If using WP
study to satisfy DMR-QA requirements, specify
that to PT Provider when ordering test samples.

❏ Send Address Verification Form (inside cover
of this package) to the State DMR-QA
Coordinator by e-mail or postal mail to confirm
receipt of Study 45 Announcement
❏ Study 45 ends

August 29,
2025

❏ Study 45 ends
❏ Send ungraded Data Report to PT Providers
(include a list of all NPDES permits using your
laboratory data)

❏ Study 45 ends

❏ PT Provider to send graded test
results, for each permit (listed by
NPDES permit numbers), to:
▪ Laboratory
▪ State DMR-QA coordinators

September
26, 2025

❏ After receipt of PT Provider-graded test
results, ensure laboratories perform retests for
any analytes graded “Not Acceptable”

❏ Forward PT Provider-graded test results to the
Permittee
❏ Order retest samples from PT Provider for all
“Not Acceptable” analyte test results. If retesting
using a WP study or quick turnaround PT sample
to satisfy a corrective action, data must be
reported to the PT Provider by the PT Provider’s
published closing date or by November 21,
2025, whichever is earlier.

October 24,
2025

❏ Send one signed copy of the NPDES Permittee
Data Report Form, copies of the PT Providergraded test results, and copies of the
Chemistry/Microbiology and WET Checklists
for each laboratory used, to the State DMRQA Coordinator

❏ Send corrective action letter including any retest
results as soon as possible to your Permittee, if
applicable

December 5,
2025

❏ Submit corrective action report including
retest results to the State DMR-QA
Coordinator, if applicable

October 10,
2025

* All materials must be sent on or before the date provided.

Page 4

DMR-QA Study 45 – Frequently Asked Questions
Am I required to participate in this Discharge Monitoring Report - Quality Assurance (DMR-QA) Study?
NPDES major and select minor permit holders are required to participate in the DMR-QA studies. Be sure to verify the NPDES permit
number on the front of this package or indicated in your e-mail. If you believe you received this study in error, contact your State DMR-QA
Coordinator (pages 10-11). Some permit holders are not required to perform the DMR-QA study because they are located in a state that
has a laboratory quality assurance program approved by EPA as a substitute for DMR-QA.2, 3, 4
What is the purpose of this DMR-QA Study?
The purpose of DMR-QA Study 45 is to ensure the integrity of data submitted by the permittee for DMR reporting requirements and evaluate
performance of the laboratories to analyze wastewater samples.
What laboratory tests are required under DMR-QA?
Permittees are responsible for having their laboratory(ies) test wastewater analytes that are both in their NPDES permit and included in
Study 45. For required Whole Effluent Toxicity (WET) tests, permittees must participate even if the test conditions (e.g., temperature, time
of acute test, synthetic seawater matrix, etc.) in the permit do not exactly match those in Study 45. Refer to “WET Testing Laboratory
Instructions” (page 8) for more information.
How should laboratory personnel perform DMR-QA tests?
For all pollutant parameters, especially field test parameters (e.g., pH, residual chlorine), use the same personnel and equipment as
required for NPDES permit compliance monitoring tests. Ensure your test methods and procedures follow 40 CFR part 136 regulations.
Where can laboratories get test samples?
Laboratories may order DMR-QA samples from a Proficiency Test (PT) Provider accredited by the American Association for Laboratory
Accreditation or the ANSI National Accreditation Board. A list of accredited PT Providers is provided on page 9.
What should permittees and laboratories do first?
Permittees must first confirm receipt of this package by July 14, 2025 to their State DMR-QA Coordinator (pages 10-11) via e-mail or
postal mail. Permittees must also send a copy of the enclosed instructions/checklists to all in-house and contract laboratories. The
permittee specifies on the checklist which analyses the laboratory will perform. See Permittee Instructions (page 6) for more information.
Upon receipt of instructions, laboratories must order samples from an accredited PT Provider (page 9). This should be done early to allow
sufficient time to perform the required analyses and send ungraded test results to the PT Provider prior to the close of DMR-QA Study 45
(August 29, 2025). See Laboratory Instructions (pages 7-8) for more information.
Can I use a Water Pollution (WP) Study to satisfy DMR-QA requirements?
Yes, permittees may use a WP study to satisfy some or all the DMR-QA requirements. However, only test results from WP studies closing
between January 1, 2025, and August 29, 2025, will be accepted. See Permittee Instructions for more information (Step 5 on page 6).
What steps do I take after the proficiency testing?
After laboratories report their data to the PT Providers, the PT Provider issues a report to the laboratory and the State DMR-QA Coordinator
indicating the test results were “Acceptable” or “Not Acceptable”. The laboratory must then forward a copy of the graded report to the
permittee. The permittee subsequently completes the checklists on pages 15-16, indicating the laboratory’s grade for each required
analyte. One set of checklists must be used for each laboratory. The permittee must also fill out the NPDES Permittee Data Report Form
(EPA Form 6400-01, pages 12-13) and submit a signed copy with the completed checklists and copies of the laboratory’s graded reports
to the State DMR-QA Coordinator by October 24, 2025.
What do I do if a laboratory receives a “Not Acceptable” test result?
If any graded test results are “Not Acceptable,” the permittee must follow up with the laboratory to determine the cause of that test result
and take corrective action to prevent future occurrences. The laboratory must order retesting samples, analyze them, and write a corrective
action response for the permittee. The permittee then submits retest results and a corrective action report to the State DMR-QA
Coordinator by December 5, 2025. Note: If the laboratory requires a retest to satisfy the corrective action, the laboratory must use a WP
study or a quick turnaround PT sample. Data must be reported to the PT Provider by the PT Provider’s published closing date or by
November 21, 2025, whichever is earlier.
Can I submit my Permittee Data Report Form electronically?
States may accept scanned, signed copies of the DMR-QA Permittee Data Report package by way of e-mail. Some states such as Arkansas
now offer electronic reporting of their DMR-QA Permittee Data Report package. States listed on pages 10 and 11 that are marked with a
dagger-style footnote (†) offer their own electronic form of the Study 45 Data Report package.
Where do I go for more information?
DMR-QA resources including fill-and-print forms are available at https://www.epa.gov/compliance/discharge-monitoring-report-qualityassurance-study-program.

2

As of April 1, 2025, permittees in the following states have laboratory quality assurance programs approved by EPA as a substitute for DMR-QA Study 45: California,
Kansas, Kentucky, New Jersey, Nevada, North Carolina, Pennsylvania, South Carolina, Utah, Virginia, West Virginia, and Wisconsin.

3

Louisiana has an approved laboratory quality assurance program for commercial laboratories only.

4

NPDES permittees in Arkansas, Maine, New Hampshire, and Oklahoma that have all their wastewater laboratories covered by their state laboratory accreditation
program are also exempted from DMR-QA Study 45; any permittee that has a laboratory not fully certified by the state must perform the DMR-QA study.
Check with your State DMR-QA Coordinator if you have questions about your state’s waiver status.

Page 5

NPDES Permittee Instructions
1.

Verify that your participation in DMR-QA Study 45 is required by confirming the NPDES permit number on the front of this package or in
the e-mail announcement sent to you. If you believe you received this study in error or have questions about your exemption status, contact
your State DMR-QA Coordinator (pages 10-11). Please refer to Footnotes 2, 3, and 4 of the Frequently Asked Questions page (page 5) for
a list of exempted states.

2.

Follow the instructions on the DMR-QA Study 45 Important Notice (inside front cover of study package) and immediately confirm receipt
of this package to your State DMR-QA Coordinator via postal mail or e-mail. You must submit your response no later than July 14, 2025. If
your permit is inactive, please contact your State DMR-QA Coordinator immediately.

3.

Send copies of these instructions to each contract and in-house laboratory, if applicable. Ensure DMR-QA samples are analyzed by the
same laboratories that routinely perform analyses for your Discharge Monitoring Report (DMR) requirements. Indicate which tests the
laboratory will perform by checking the appropriate boxes in the “Test Required” column in the enclosed tables (pages 15-16).

4.

Ensure that each laboratory uses their U.S. EPA Laboratory Code on all reported test results. Make certain your laboratories understand
and complete all requirements. Laboratories needing a new U.S. EPA Lab Code or wanting to confirm their existing U.S. EPA Lab Code,
should contact their EPA Regional DMR-QA Coordinator listed on page 9. Submit all requests for lab codes at least one week before the
DMR-QA Study 45 end date (August 29, 2025) or one week prior to the PT Provider’s WP study due date to allow time for response.

5.

Instruct your laboratory to order samples for analytes that are both in your permit and included in DMR-QA Study 45. Your in-house and
contract laboratories must order PT samples from an accredited PT Provider (page 9). Ensure your laboratory orders the samples early
enough to allow time to perform the required analyses and send test results to the PT Provider prior to the close of DMR-QA Study 45
(August 29, 2025). Your laboratory should register your permit number with the PT Provider prior to August 29, 2025. Note: If your inhouse or contract laboratory chooses to use a WP study to satisfy DMR-QA requirements, please inform the laboratory that all data must
be reported to the PT Provider by the published WP study close date, even if it is prior to the DMR-QA deadline of August 29, 2025. WP
studies are only valid for DMR-QA Study 45 if the WP study meets the following requirements:
a.

Samples are offered by an accredited PT Provider (page 9).

b.

The WP study closed between January 1, 2025, and August 29, 2025.

c.

The PT Provider shows the WP test results from each of the permittee’s regulated analytes on the DMR-QA reporting form.

6.

Permittees are responsible for ensuring that laboratories submit data on the permittee’s behalf to the PT Provider by the end of DMR-QA
Study 45 (August 29, 2025). Laboratories must send ungraded data to the same PT Provider that provided the samples to the laboratory.
By September 26, 2025, PT Provider-graded test results will be sent back to the laboratories. Permittees will not receive graded reports
directly from PT Providers unless they are an in-house laboratory. Note: Permittees are not required to report ungraded data to the PT
Provider on their laboratories’ behalf nor should permittees send ungraded data to their State DMR-QA Coordinator.

7.

Permittees must require laboratories to forward the PT Provider-graded test results to them by October 10, 2025. Using these graded test
results, permittees must fill out the Chemistry/Microbiology Analyte and WET Analyte checklists (pages 15-16) for each laboratory,
indicating the analyte tests performed by the laboratory and whether the test result was “Acceptable” or “Not Acceptable.” Make sure the
appropriate NPDES permit number and U.S. EPA Lab Code are on each checklist. If you use more than one laboratory, you must use a
separate checklist for each laboratory. If a laboratory reports more than one test method to you for any single analyte, you must use a
separate checklist for each test method reported.

8.

Follow the directions on the “NPDES Permittee Data Report Form” (Form 6400-01, pages 12-14) and complete the information. You may
use a “fill and print” form available at: https://www.epa.gov/compliance/discharge-monitoring-report-quality-assurance-study-program. By
October 24, 2025, you must send a copy of the signed Form 6400-01, graded test results, and completed checklists from step 7 for each
of your permits to the State DMR-QA Coordinator.

9.

a.

However, if you (the Permittee) have verified that the PT Provider’s graded test results have been transmitted to the State DMRQA Coordinator, you are only required to submit the signed Form 6400-01 and the applicable checklists to the State DMR-QA
Coordinator. In this situation, the laboratory should provide a list of its permittees’ NPDES permit numbers associated with each
required analyte to the PT Provider prior to the close of DMR-QA Study 45 or the WP study. Check with your laboratory to determine
whether the PT Provider is sending the graded data directly to the State DMR-QA Coordinator.

b.

Permittees must maintain a copy of the completed NPDES Permittee Data Report Form, checklists, and graded laboratory results
as a record for at least three (3) years.

After receiving the PT Provider-graded test results, permittees must consult with the laboratory and investigate all test results graded by
the PT Provider as “Not Acceptable.” Permittees must identify and report to the State DMR-QA Coordinator the causes of any discrepancies
and must describe the analytical system changes required to correct the discrepancies. If the laboratory requires a retest to satisfy the
corrective action, the laboratory must use a WP study or a quick turnaround PT sample. Data must be reported to the PT Provider by the
PT Provider’s published closing date or by November 21, 2025, whichever is earlier. Laboratories must order retesting samples by October
10, 2025, for any “Not Acceptable” results and perform the retests as soon as possible. The corrective action report should include results
from any retest/verification analysis performed and must be sent to the State DMR-QA Coordinator on or before December 5, 2025.

Page 6

Chemistry/Microbiology Laboratory Instructions
Your laboratory is designated to participate in DMR-QA Study 45 by a NPDES permittee because the permittee uses or plans to use
your laboratory to perform chemistry/microbiology analyses to satisfy their NPDES permit requirements during 2025. For
measurements of all pollutant parameters, especially field test parameters (pH, residual chlorine, etc.), use the same personnel
and equipment as required for NPDES compliance monitoring analyses. Please ensure that your test methods/procedures follow
40 CFR part 136 regulations. Please note that for low level mercury, the concentration range is 20 - 100 ng/L (20 - 100 parts per
trillion) and for low level total residual chlorine, the concentration range is 75 - 250 µg/L (75 - 250 parts per billion). If you have
questions about whether you should perform the test using the normal or the low-level mercury and/or low-level total residual
chlorine concentration, contact your State DMR-QA Coordinator.
1. Each permittee for whom you analyze or provide data in 2025 will determine which analyses you must perform by checking the
appropriate boxes on the enclosed “Chemistry/Microbiology Analyte Checklist” (page 15).
2. Order DMR-QA Study 45 chemistry and microbiology samples from one of the Proficiency Test (PT) Providers (page 9). Be sure
to allow yourself enough time to perform the analyses before the closing date of DMR-QA Study 45 (August 29, 2025). Maintain
a copy of all completed order forms for your records.
Note: You may be able to utilize the test results from a Water Pollution (WP) study to meet the requirements of DMR-QA
Study 45, if all the following conditions are met:
a. Samples are offered by an accredited PT Provider (page 9).
b. The WP study closes between January 1, 2025, and August 29, 2025.
c. The PT Provider shows the WP study results of each permittee’s regulated analytes on the DMR-QA reporting form.
3. Each reported value must be produced from a single analytical run using the analytical system that routinely performs these
analyses to produce compliance monitoring data required under your permittee’s NPDES permit. Your results must not be
compared with results from other independent analyses conducted by you or any other laboratory before reporting your test
results to the PT Provider.
4. Record your ungraded analytical data and your EPA Lab Code on the Data Reporting Forms that you received with your test
samples. Be sure to follow the PT Provider’s instructions and deadlines that you received with these samples.
5. You are required to use the EPA-assigned Lab Code on all reported test results. If you need a new EPA Lab Code or need to
verify your existing EPA Lab Code, please contact your EPA Regional DMR-QA Coordinator (see contact information on page
9).
6. Send the data requested by each of your permittees to the PT Provider for grading. You must send data to the same PT Provider
that sent you the test samples. Make sure you provide the DMR-QA Study 45 or WP study results to the PT Provider by August
29, 2025. If you choose to use a WP study, you must report all data to the PT Provider by the PT Provider’s published WP study
close date, even if it is prior to the DMR-QA deadline of August 29, 2025. Notify the PT Provider that the WP study is being
used to satisfy DMR-QA Study 45 requirements and send them copies of the analyte checklist(s) if you are reporting via hard
copy.
7. The PT Provider will grade your analyses and send the graded test results to you by September 26, 2025. If the PT Provider is
submitting graded test results sorted by permit number to the State DMR-QA Coordinator, then you should register your
permittees’ permit numbers associated with each required analyte with the PT Provider prior to the close of DMR-QA Study 45
or the WP study. Forward the graded test results of the analytes to the permittee by October 10, 2025, so the permittee can fill
out the analyte checklist on page 15.
8. If any graded test results are “Not Acceptable,” laboratories should coordinate with the permittee to determine the cause of
that test result and identify corrective action to prevent future occurrences. If the laboratory requires a retest to satisfy the
corrective action, the laboratory must use a WP study or a quick-turnaround PT sample. Data must be reported to the PT
Provider by the PT Provider’s published closing date or by November 21, 2025, whichever is earlier. Laboratories must order
retest samples for “Not Acceptable” analyte test results by October 10, 2025. The corrective action report and graded retest
results must be forwarded to the permittee as soon as possible, for the permittee is required to submit a completed corrective
action report to their state DMR-QA coordinator by December 5, 2025.

Page 7

WET Testing Laboratory Instructions
Your laboratory is designated to participate in DMR-QA Study 45 by a NPDES permittee because the permittee uses or plans to use your
laboratory to perform Whole Effluent Toxicity (WET) analyses during 2025. For all analytes, use the same personnel and equipment as required
for NPDES permit compliance monitoring analyses. Labs should ensure that WET test methods/procedures follow instructions from your PT
Provider and EPA’s WET test manuals referenced below, which include both EPA’s promulgated WET test methods at 40 CFR part 136 and
EPA’s recommended short-term chronic marine West Coast WET test methods (1995).
1.

The permittee(s) determine which analyses you will perform by checking the appropriate boxes on the enclosed WET Analyte checklist
(page 16). Labs should ensure that the permittee has selected the test organism(s), test conditions, and testing requirements that most
closely resemble those required by the permit for which you are supplying test results. Use the guidelines immediately below and the
table on page 16 to select the proper WET tests.
•
•

•

•
•
•
•

Guidelines for Choosing the Correct WET Test Organism/Conditions/Test Endpoint(s):
Laboratories should only report one test endpoint for each DMR-QA WET test code required.
For laboratory performance quality assurance purposes only, the point estimation techniques that produce test endpoints such
as the Inhibiting Concentration 25% (IC25) are the preferred statistical approach used for calculating test endpoints for effluent
chronic toxicity tests. However, laboratories should choose the statistical approach that allows calculation of the test endpoint(s)
required by the NPDES permit and are used for routine permit compliance tests. For example, if the permit specifies a No
Observable Effect Concentration (NOEC) test endpoint for Survival and it is reported regularly on DMRs, this test endpoint may
be reported for DMR-QA.
If the permit requires WET testing with the Fathead minnow (Pimephales promelas), Ceriodaphnia dubia, Daphnia magna, Daphnia
pulex, Americamysis bahia (Mysidopsis bahia), Inland silverside (Menidia beryllina) or Sheepshead minnow (Cyprinodon
variegatus), the laboratory must test with those test organisms listed in each permit using the test conditions, including
temperature, defined in the WET Test Codes.
If the permit’s WET testing conditions for Ceriodaphnia dubia specify 48-hr acute static renewal testing, then conduct this test
using the static non-renewal conditions specified in WET Test Codes 19 and 20.
If the permit’s WET testing conditions for Daphnia magna and Daphnia pulex specify 48-hr acute static renewal testing, then
conduct this test using the static non-renewal conditions specified in WET Test Codes 32 and 38.
If the permit’s WET testing conditions require 24-, 48-, or 96-hr acute testing using any of the test organisms included in Study
45, use the 48-hr acute test conditions specified in the WET Test Codes.
If the permit requires 20°C acute testing for any test organisms included in Study 45, use 25°C acute test conditions specified in
the WET Test Codes.

2.

Order DMR-QA toxicity samples from an accredited PT Provider (page 9). Allow yourself enough time to perform the analyses before the
closing date of DMR-QA Study 45 (August 29, 2025). Maintain a copy of all completed order forms for your records.

3.

Each reported value must be produced from a single analytical run using the analytical system that routinely performs these analyses to
produce compliance monitoring data required under your permittee’s NPDES permit. Your results must not be compared with results
from other independent analyses conducted by you or any other laboratory before reporting your test results to the PT Provider.

4.

Record your ungraded analytical data and EPA Lab Code on the Data Report Form received with your samples. Be sure to follow the PT
Provider’s instructions and deadlines that you received with the samples. You must use the EPA-assigned Lab Code on all reported test
results. If you need a new EPA Lab Code or need to verify your current code, contact your EPA Regional DMR-QA Coordinator (page 9).

5.

Send the ungraded data requested by each of your permittees to the PT Provider for grading. You must send it to the same PT Provider
that sent you the test samples. Make sure you provide the DMR-QA Study 45 study results by August 29, 2025.

6.

The PT Provider will grade your test results and send them to you by September 26, 2025. If the PT Provider is submitting graded test
results sorted by permit number to the State DMR-QA Coordinator, then you should register your permittees’ permit numbers associated
with each required analyte with the PT Provider prior to the close of DMR-QA Study 45 or the WP study. Forward the graded test results
of the analytes to the permittee by October 10, 2025, so the permittee can fill out the analyte checklist on page 16.

7.

If any graded test results are “Not Acceptable,” laboratories should coordinate with the permittee to determine the cause of the result
and identify corrective action to prevent future occurrences. If the laboratory requires a retest to satisfy the corrective action, the
laboratory must use a WP study or a quick turnaround PT sample. Data must be reported to the PT Provider by their published closing
date or by November 21, 2025, whichever is earlier. Laboratories must order retest samples for “Not Acceptable” analyte test results by
October 10, 2025. The corrective action report and graded retest results must be forwarded to the permittee as soon as possible, for the
permittee is required to submit a completed corrective action report to their state DMR-QA coordinator by December 5, 2025.

Reference Manuals: (see https://www.epa.gov/cwa-methods/whole-effluent-toxicity-methods)
1. Methods for Measuring the Acute Toxicity of Effluents to Freshwater and Marine Organisms, Fifth Edition, October 2002. U.S. Environmental Protection Agency,
Office of Water, Washington, DC, EPA 821-R-02-012.
2. Short-term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, Fourth Edition, October 2002. U.S.
Environmental Protection Agency, Office of Water, Washington, DC, EPA 821-R-02-013.
3. Short-term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Marine and Estuarine Organisms, Third Edition, October 2002. U.S.
Environmental Protection Agency, Office of Water, Washington, DC, EPA 821-R-02-014.
4. Method Guidance and Recommendations for Whole Effluent Toxicity (WET) Testing (40 CFR Part 136). July 2000. U.S. Environmental Protection Agency, Office of
Water, Washington, DC, EPA-821-B -00-004.
5. Short-term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to West Coast Marine and Estuarine Organisms, 1st Ed., 1995, U.S.
Environmental Protection Agency, Office of Research and Development, Cincinnati, OH, EPA/600/R-95/136.
https://cfpub.epa.gov/si/si_public_record_report.cfm?dirEntryId=46584.

Page 8

Accredited Proficiency Testing Providers
The following Proficiency Test (PT) Providers are accredited by the American Association for Laboratory Accreditation (A2LA) or the ANSI
National Accreditation Board (ANAB). A2LA and ANAB have each been designated a Proficiency Testing Oversight Body/Proficiency
Testing Provider Accreditor by The NELAC Institute (TNI). Find the following PT Providers on the internet at: http://www.nelacinstitute.org/content/NEPTP/ptproviders.php.
NELAC-Accredited Provider
Absolute Standards, Inc., Hamden, CT
Mr. Stephen Arpie (203) 281-2917 or (800) 368-1131
[email protected]
www.absolutestandards.com
Advanced Analytical Solutions, LLC, Parkersburg, WV
Fred Anderson (304) 485-6325
[email protected]
www.advancedqa.com
Environmental Resource Associates (ERA), Golden, CO
(800) 372-0122
[email protected]; [email protected]
www.eraqc.com
New York State Department of Health Wadsworth Center, Albany, NY
Amy C. DeMarco (518) 473-1398
[email protected]
www.wadsworth.org/programs/ehs/pt
NSI Lab Solutions, Raleigh, NC
Kenneth Grzybowski (800) 234-7837
[email protected]
www.nsilabsolutions.com
Phenova Inc., Golden, CO
(866) 942-2978
[email protected]
www.phenova.com

Chem

✔
✔
✔
✔
✔
✔

Micro

✔
✔
✔
✔
✔
✔

WET

✔

✔

EPA Regional DMR-QA Coordinators
EPA Region 1
(CT, MA, ME, NH, RI, VT)
Nicole Paradise
U.S. EPA Region 1
LSASD/LQAB
11 Technology Drive
North Chelmsford, MA 01863-2431
(617) 918-8331
[email protected]

EPA Region 4
(AL, FL, GA, KY, MS, NC, SC, TN)
Jeffrey Wilmoth
U.S. EPA Region IV
LSASD/QAPSB/QAS
980 College Station Rd
Athens, GA 30605-2720
(706) 355-8623
[email protected]

EPA Region 7 (IA, KS, MO, NE)
Greg Savitske (c/o Jodi Bruno)
U.S. EPA Region VII
ECAD/WB
11201 Renner Blvd
Lenexa, KS 66219
(202) 564-2601
[email protected]

EPA Region 9
(AS, AZ, CA, GU, HI, MP, MW, NN, NV)
Adam Howell
U.S. EPA Region IX
ECAD/WB/WS1
75 Hawthorne St (ENF-3-1)
San Francisco, CA 94105
(415) 972-4248
[email protected]

EPA Region 2 (NJ, NY, PR, VI)
Molly Hillenbrand
U.S. EPA Region II
LSASD/MAB/MOS
2890 Woodbridge Ave (MS 220)
Edison, NJ 08837
(732) 321-4452
[email protected]

EPA Region 5 (IL, IN, MI, MN, OH, WI)
Kenneth Gunter
U.S. EPA Region V
ECAD/WECAB/WS1
77 W. Jackson Blvd (ECW-15J)
Chicago, IL 60604
(312) 353-9076
[email protected]

EPA Region 8 (CO, MT, ND, SD, UT, WY)
Lisa-kay Prideaux
U.S. EPA Region VIII
ECAD/WEB/NWES
10 West 15th Street (8MO)
Helena, MT 59626
(406) 457-5022
[email protected]

EPA Region 10 (AK, ID, OR, WA)
Jason Rodriguez
U.S. EPA Region X
ECAD/WEFB/SWES
1200 Sixth Ave
Seattle, WA 98101
(206) 553-8508
[email protected]

EPA Region 3 (DC, DE, MD, PA, VA, WV) EPA Region 6 (AR, LA, NM, OK, TX)
Peter Gold
Mariela Reyna
U.S. EPA Region III
U.S. EPA Region VI
ECAD/WB/NPDES
ECAD/WTREB/WRS
1650 Arch St (3ED32)
1201 Elm St, Suite 500 (6ECDWR)
Philadelphia, PA 19103
Dallas, TX 75270
(215) 814-5236
(214) 665-7177
[email protected]
[email protected]

Trying to find your
State DMR-QA Coordinator?
Turn to pages 10 and 11 ➜

Page 9

State DMR-QA Coordinators
Alabama
Jocelyn B. Moore
Alabama Dept. of Environmental Mgmt.
OEQ-QSU
PO Box 301463
Montgomery, AL 36130-1463
(334) 260-2739
[email protected]

Delaware
Nicole Smith
Delaware DNREC - Division of Water
Surface Water Discharges Section
89 Kings Hwy - R and R Building
Dover, DE 19901
(302) 739-9946
[email protected]

Indiana
Maggie Kroeger
Indiana DEM/Office of Water Quality
100 North Senate Avenue
MC 65-42, IGCN N1255
Indianapolis, IN 46204-2251
(317) 619-3639
[email protected]

Michigan
Claire Handley
Michigan EGLE
Scanned Mail - IMD
PO Box 30457
Lansing, MI 48909-7957
(231) 878-6795
[email protected]

Alaska
Melinna Faw
Alaska DEC - Division of Water
555 Cordova St
Anchorage, AK 99501-2617
(907) 269-3066
[email protected]

District of Columbia
Robert Burnett
District of Columbia Dept. of Energy &
Environment - Water Quality Division
1200 First Street NE, 6th Floor
Washington, DC 20002
(202) 578-6237
[email protected]

Iowa
Greg Savitske (c/o Jodi Bruno)
U.S. EPA Region VII, ECAD/WB
11201 Renner Blvd
Lenexa, KS 66219
(202) 564-2601
[email protected]

Minnesota
Jennifer Thoresen
Minnesota Pollution Control Agency
Environmental Outcomes Division
520 Lafayette Road
St. Paul, MN 55155
(651) 757-2805
[email protected]

American Samoa
Adam Howell
U.S. EPA Region IX
ECAD/WB/WS1
75 Hawthorne St (ENF-3-1)
San Francisco, CA 94105
(415) 972-4248
[email protected]
Arizona
Michael Tenczar
AZPDES Individual Permits Unit
Arizona Dept of Environmental Quality
1110 W. Washington St
Phoenix, AZ 85007
(602) 771-4623
[email protected]
Arkansas*†
Yvette Wilkins
Arkansas Department of Energy
and Environment
5301 Northshore Drive
North Little Rock, AR 72118
(501) 682-0941
[email protected]
https://eportal.adeq.state.ar.us/

Florida
William Joyner
U.S. EPA Region IV, ECAD/WEB/WES
61 Forsyth St SW, MC-9T25
Atlanta, GA 30303-8960
(404) 562-8795
[email protected]
Georgia
Sam Wright
Georgia DNR
Environmental Protection Division
Watershed Protection Branch
2 MLK Jr. Dr., S.W., Suite 1152 East
Atlanta, GA 30334
(470) 524-1643
[email protected]

Kansas*
Paul Harrison
Kansas Dept. of Health & Environment
Kansas Health and Environmental
Laboratories
6810 SE Dwight Street
Topeka, KS 66620
(785) 296-6198
[email protected]
Kentucky*
Patrick J. Garrity
Laboratory Certification Officer
Kentucky DEP - Division of Water
300 Sower Blvd, 3rd Floor
Frankfort, KY 40601
(502) 782-6954
[email protected]

Guam
Maricar Quezon
Water Pollution Control Program
Guam EPA
P.O. Box 22439
Barrigada, GU 96921
(671) 475-1601
[email protected]

Louisiana*
Sandy Coon
Louisiana DEQ
Office of Environmental Compliance
P.O. Box 4312
Baton Rouge, LA 70821-4312
(225) 219-3614
[email protected]

California*
Andrew Hamilton
Office of Information Mgmt & Analysis
State Water Resources Control Board
1001 I Street, 16-39D
Sacramento, CA 95814
(916) 341-5286
[email protected]

Hawaii
Randee Tubal
Hawaii Department of Health
Clean Water Branch
2827 Waimano Home Road, Room 225
Pearl City, HI 96782
(808) 586-4309
[email protected]

Maine*
Brett Goodrich
Maine Dept. of Environmental Protection
Division of Water Quality Management
17 State House Station
Augusta, ME 04333
(207) 287-9034
[email protected]

Colorado
Jocelyn Brink
Colorado Dept. of Public Health and
Environment
Water Quality Control Division-CWE-B2
4300 S Cherry Creek Drive South
Denver, CO 80246
(303) 692-2840
[email protected]

Idaho
Jason Rodriguez
U.S. EPA Region X
ECAD/WEFB/SWES
1200 Sixth Ave
Seattle, WA 98101
(206) 553-8508
[email protected]

Maryland
Maryland Dept. of the Environment
Water Management Administration
Compliance Program
1800 Washington Blvd, Suite 420
Baltimore, MD 21230
[email protected]

Connecticut
Chris Chaber
Connecticut DEEP
Bureau of Materials Management and
Compliance Assurance
79 Elm Street
Hartford, CT 06106
(860) 424-3293
[email protected]
*
†

Illinois
Michelle Rousey
Illinois EPA
Bureau of Water
1021 North Grand Avenue East
P.O. Box 19276 (Mail Code 15)
Springfield, IL 62794-9276
(217) 785-3944
[email protected]

Massachusetts
Ping Yee
Massachusetts DEP/Boston Office
100 Cambridge St, Suite 800
Boston, MA 02114
(617) 634-9744
[email protected]

Mississippi
Beverly Ashmore-Bates
Mississippi DEQ
Office of Pollution Control
1542 Old Whitfield Road
Pearl, MS 39208
(601) 961-5737
[email protected]
Missouri
Greg Savitske (c/o Jodi Bruno)
U.S. EPA Region VII, ECAD/WB
11201 Renner Blvd
Lenexa, KS 66219
(202) 564-2601
[email protected]
Montana
Gina Self
Montana DEQ
Water Protection Bureau
P.O. Box 200901
Helena, MT 59620-0901
(406) 444-5388
[email protected]
Nebraska
Phillip Halsted
Nebraska Department of Environment
and Energy
Water Quality Division
P.O. Box 98922
Lincoln, NE 68509
(402) 471-1367
[email protected]
Nevada*
Donald E. LaFara
Bureau of Safe Drinking Water
Nevada Division of Environmental
Protection
901 S. Stewart Street, Suite 4001
Carson City, NV 89701
(775) 687-9491
[email protected]

(List continues next page ➜)

Some or all permittees in these states may not be required to participate in DMR-QA Study 45 due to a full or partial waiver agreement with EPA.
Some or all permittees in Arkansas can now submit their DMR-QA Study 45 results electronically through Arkansas DEQ’s ePortal website (see website link, above).

Page 10

New Hampshire*
Stephanie Larson
New Hampshire DES - Water Division
P.O. Box 95
Concord, NH 03302-0095
(603) 271-1493
[email protected]
New Jersey*
Department of Environmental Protection
Office of Quality Assurance
Laboratory Certification Programs
P.O. Box 402, Mail Code 401-02D
Trenton, NJ 08625-0402
(609) 777-1749
New Mexico
Jason Martinez
New Mexico Environment Department
Surface Water Quality Bureau
Harold Runnels Bldg, N 2050
1190 South St. Francis Drive (8750S)
P.O. Box 5469
Santa Fe, NM 87505-5469
(505) 372-8376
[email protected]
New York
Molly Hillenbrand
U.S. EPA Region II, LSASD/MAB/MOS
2890 Woodbridge Ave (MS 220)
Edison, NJ 08837
(732) 321-4452
[email protected]
North Carolina*
Todd Crawford
North Carolina DEQ
Division of Water Resources
Laboratory Certification Branch
1623 Mail Service Center
Raleigh, NC 27699-1623
(919) 733-3908, ext. 251
[email protected]
North Dakota
Marty Haroldson
North Dakota DEQ
Division of Water Quality
Permits Program
918 East Divide Ave., 4th Floor
Bismarck, ND 58501
(701) 328-5234
[email protected]

Northern Mariana Islands
Adam Howell
U.S. EPA Region IX
ECAD/WB/WS1
75 Hawthorne St (ENF-3-1)
San Francisco, CA 94105
(415) 972-4248
[email protected]

Rhode Island
Matthew Puglia, PE
Rhode Island DEM
Office of Water Resources
235 Promenade Street
Providence, RI 02908
(401) 537-4153
[email protected]

Ohio
Steve Roberts
Ohio EPA
8955 East Main Street
Reynoldsburg, OH 43068
(614) 644-4225
[email protected]

South Carolina*
Bennie L. Cockerel
Office of Environmental Laboratory
Certification
South Carolina DES
2600 Bull St
Columbia, SC 29201
(803) 896-0970
[email protected]

Oklahoma*
Lynzie Chan
Oklahoma DEQ
P.O. Box 1677
Oklahoma City, OK 73101
(405) 702-8243
[email protected]
Oregon
Travis Bartholomew
Oregon DEQ
Environmental Laboratory Accreditation
Program
7202 NE Evergreen Parkway, Ste 100
Hillsboro, OR 97124
(971) 300-3249
[email protected]

South Dakota
Tim Flor
South Dakota Dept of
Environment & Natural Resources
Surface Water Quality Program
523 East Capital
Pierre, SD 57501
(605) 773-3351
[email protected]

Tennessee
Barbara Loudermilk
Division of Water Resources
Compliance & Enforcement Unit
Davy Crockett Tower, 9th Floor
500 James Robertson Pkwy
Pennsylvania*
Nashville, TN 37243
Department of Environmental Protection (615) 313-5742 or (615) 686-9755
Bureau of Laboratories
[email protected]
Attn: Laboratory Accreditation Program
P.O. Box 1467
Texas
Harrisburg, PA 17105-1467
Sagar Shrestha
[email protected]
Texas Commission on Environmental
Quality
Puerto Rico
P.O. Box 13087, MC 165
Molly Hillenbrand
Austin, TX 78711
U.S. EPA Region II, LSASD/MAB/MOS
(512) 239-3307
2890 Woodbridge Ave (MS 220)
[email protected]
Edison, NJ 08837
(732) 321-4452
Utah*
[email protected]
Lisa-kay Prideaux
U.S. EPA Region VIII
ECAD/WEB/NWES
10 West 15th Street (8MO)
Helena, MT 59626
(406) 457-5022
[email protected]

Vermont
Michelle Kolb
Vermont DEC
1 National Life Dr. - Bldg Main 2
Montpelier, VT 05620-3522
(802) 490-6165
[email protected]
Virgin Islands
Molly Hillenbrand
U.S. EPA Region II, LSASD/MAB/MOS
2890 Woodbridge Ave (MS 220)
Edison, NJ 08837
(732) 321-4452
[email protected]
Virginia*
DCLS Laboratory Certification Office
600 N. 5th Street
Richmond, VA 23219
(804) 648-4480
[email protected]
Washington
Rebecca Wood
Washington State Dept. of Ecology
Laboratory Accreditation Unit
P.O. Box 488
Manchester, WA 98353
(360) 871-8811
[email protected]
West Virginia*
Justin Carpenter
West Virginia DEP
601 57th Street, S.E.
Charleston, WV 25304
(304) 926-0499, ext. 43765
[email protected]
Wisconsin*
Wisconsin DNR
Laboratory Certification Program - SS/7
PO Box 7921
101 S Webster St.
Madison, WI 53707
(608) 264-6006
[email protected]
Wyoming
Steve E. Vien, PhD
Wyoming DEQ
Water Quality Division
208 South College Drive
Cheyenne, WY 82002
(307) 777-7654
[email protected]

Your coordinator may change.
Please
visit
https://www.epa.gov/compliance/state-discharge-monitoring-report-quality-assurance-dmr-qa-coordinators
and
https://www.epa.gov/compliance/regional-discharge-monitoring-report-quality-assurance-dmr-qa-coordinators for the latest list of
coordinators.
For additional questions, contact US EPA Headquarters:
Greg Savitske
USEPA/OECA/OC/MAMPD
1200 Pennsylvania Ave NW
Mail Code 2227A
Washington, DC 20460
(202) 564-2601
[email protected]

*

Some or all permittees in these states may not be required to participate in DMR-QA Study 45 due to a full or partial waiver agreement with EPA.

Page 11

United States Environmental Protection Agency
Office of Enforcement and Compliance Assurance
DMR-QA Study 45

OMB Control No. 2080-0021
Approval expires 08/31/2026

(This data is collected under the authority of Section 308 of the Clean Water Act.)

2025

Instructions for the NPDES Permittee Data Report Form:
1. This is a two-page form. All fields on page 13 are required to be reported except for those explicitly marked
as optional.
2. Enter your NPDES permit number at the top of pages 13 and 14.
3. You must fill in the 2-digit permit extension field at the top of page 13 if there is an extension for your permit
code. If you have one, the extension will appear next to your permit code in the address box on page 13; for
example: “NPDES Permittee AL1234567-01.” If there is no extension, leave this field blank.
4. Identify each of your laboratories on page 14, including their U.S. EPA Lab Code which is a unique identifier
number assigned by EPA. (Refer to page 6, item 4 in the Study 45 package) (NOTE: The U.S. EPA Lab Code
of the laboratory that produced the data must also appear at the top of the Chemistry/ Microbiology and
WET analyte checklists on pages 15-16.)
5. Make copies of pages 13 and 14. Attach a copy of these pages to the Chemistry/Microbiology and WET
analyte checklists. Separate copies of each checklist must be filled out for each laboratory you used. Also,
if a laboratory reports more than one test method to you for any single analyte, you must use a separate
checklist for each test method reported. These checklists must indicate the graded test results for the
analytes tested by the laboratory that are in your permit and required for DMR-QA (Acceptable or Not
Acceptable). For Study 45, it is optional but encouraged to write in the approved EPA test method used for
DMR- QA Chemistry/Microbiology analytes. If you use a state-certified laboratory to generate your NPDES
data in a state that has been granted a partial exemption from the DMR-QA study by the EPA Region, check
the “Analyte determined by state-certified laboratory” box(es) on the checklists (pages 15 and 16) for all
analytes in your permit analyzed by a state-certified laboratory.
6. Sign and date the certification statement on page 13 and the applicable checklists on pages 15 and 16.
Forms that do not have a signed certification statement by the permit holder or authorized representative
will be considered invalid.
7. Make copies of the NPDES Permittee Data Report form and checklists for your records.
8. Submit the signed copy of the Permittee Data Packages by e-mail or postal mail to the State DMR-QA
Coordinator no later than October 24, 2025.
Paperwork Reduction Act Burden Statement. This collection of information is approved by OMB under the Paperwork Reduction
Act, 44 U.S.C. 3501 et seq. (OMB Control No. 2080-0021). Responses to this collection of information are mandatory for Major and
select Minor NPDES permit holders under Clean Water Act section 308 [33 U.S.C. § 1318]. An agency may not conduct or sponsor,
and a person is not required to respond to, a collection of information unless it displays a currently valid OMB control number. The
public reporting and recordkeeping burden for this collection of information is estimated to average 6.6 hours per response. Send
comments on the Agency’s need for this information, the accuracy of the provided burden estimates, and any suggested methods for
minimizing respondent burden to the Regulatory Support Division Director, U.S. Environmental Protection Agency (2821T), 1200
Pennsylvania Ave NW, Washington, DC 20460. Include the OMB control number in any correspondence. Do not send the completed
form to this address.

EPA Form 6400-01 (Rev. 0-25). Previous editions are obsolete.

Page 12

OMB Control No. 2080-0021
Approval expires 08/31/2026

United States Environmental Protection Agency
Office of Enforcement and Compliance Assurance
DMR-QA Study 45

2025

(This data is collected under the authority of Section 308 of the Clean Water Act.)

NPDES Permittee Data Report Form

Attention: Follow the instructions on the previous page to complete this form and submit data for evaluation.
NPDES Permit Number (State + 7-digit ID)

Due October 24, 2025

Permit Extension

Permittee Name
Current Permittee Mailing Address
City

State

Phone Number

Fax Number

Zip Code

E-mail Address

Optional: If WP Study was used, list PT Provider name(s):
Optional: IF WP Study was used, list WP Study Number(s):
For DMR-QA Study 45, conducted in 2025, the Permittee ensured that their laboratory(ies) performing the required analyses:
Submitted Complete and Accurate Data
Received a Graded Report by
Received PT Samples
by August 29, 2025
September 26, 2025
YES ❏

NO ❏

YES ❏

Each reported value was produced from a single analytical run
using the analytical system that routinely performs these
analyses to produce compliance monitoring data under our
NPDES permit.
YES ❏

NO ❏

YES ❏

NO ❏

Neither I nor any of my subordinates compared our results with
results from independent analyses conducted by us or any other
laboratory before we reported our results to U.S. EPA.

NO ❏

YES ❏

NO ❏

Certification by Permit Holder or Authorized Representative
I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance
with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my
inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the
information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant
penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.
Name of Certifying Official

Title

Signature

Date

Address, phone number and e-mail of certifying official are required if different from above.
Address
City

Phone Number
State

EPA Form 6400-01 (Rev. 0-25). Previous editions are obsolete.

Zip Code

E-mail Address

Page 13

OMB Control No. 2080-0021
Approval expires 08/31/2026

United States Environmental Protection Agency
Office of Enforcement and Compliance Assurance
DMR-QA Study 45

2025

(This data is collected under the authority of Section 308 of the Clean Water Act.)

Permittee Name

NPDES Permit Number (State + 7-digit ID)

Permit Extension

Identification of all CHEM, MICRO and WET laboratories who performed analyses for this permit
Laboratory Name

U.S. EPA Lab
Code

Laboratory Address

Lab Analysis
Check box(es) that apply

Lab
Type*

Statecertified
Lab**

Chem

Micro

WET

❏

❏

❏

❏

❏

❏

❏

❏

❏

❏

❏

❏

❏

❏

❏

❏

❏

❏

❏

❏

❏

❏

❏

❏

* Lab Types: C = Commercial; F = Federal; G = Local Government; I = Industrial; O = Other; S = State
** See Footnotes 2, 3, and 4 on page 5 (Frequently Asked Questions) for the current list of states with lab accreditation programs
If you need additional space, please make a copy of this page for additional laboratories.

EPA Form 6400-01 (Rev. 0-25). Previous editions are obsolete.

Page 14

Permittee Name

NPDES Permit No.

EPA Lab Code

Chemistry/Microbiology Analyte Checklist

2025

DMR-QA Study 45
Analyte Test
Microbiology
E. coli, MF or MPN
Fecal Coliform, MF or MPN
Total Coliform, MF or MPN
Trace Metals
Aluminum
Antimony
Arsenic
Barium
Beryllium
Cadmium
Chromium, total
Chromium, hexavalent
Cobalt
Copper
Iron
Lead
Manganese
Mercury
Mercury (Low-Level)
Molybdenum
Nickel
Selenium
Silver
Thallium
Vanadium
Zinc
Demands
5-day BOD
5-day Carbonaceous BOD
COD
TOC
Minerals
Alkalinity, total (CaCO3)
Chloride
Fluoride
Hardness, total (CaCO3)
Specific conductance (25°C)
Sulfate
Total Dissolved Solids (180°C)
Nutrients
Ammonia as N
Nitrate as N
Nitrite as N
Orthophosphate as P
Total Kjeldahl-Nitrogen as N
Total Phosphorus as P
Misc. Analytes
Non-Filterable Residue (TSS)
Oil and Grease
pH
Total Cyanide
Total Phenolics (4-AAP)
Total Residual Chlorine
Total Residual Chlorine (Low-Level)
Settleable Solids
Turbidity

Test
Required

Method Number Used
(Optional)

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——————

Name
* See Footnotes 2 through 4 on page 5

——————

——————

——————

——————

——————

Signature

Laboratory’s Graded Result
Not Acceptable
Acceptable
(Corrective Action
Required)

———————
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———————
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Analyte
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state-certified
lab*

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Date
Page 15

Complete a separate checklist for EACH lab.

Permittee Name

NPDES Permit No.

EPA Lab Code

Whole Effluent Toxicity (WET) Analyte Checklist
DMR-QA Study 45
Analyte
Number

Organism / Conditions

Test Code 13 (refer to EPA Method 2000.0)
754

Fathead minnow (Pimephales promelas) - MHSF 25°C

Test Code 14 (refer to EPA Method 2000.0)
755

Fathead minnow (Pimephales promelas) - 20% DMW

Test Code 15 (refer to EPA Method 1000.0)

Endpoint

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LC50

———————
LC50

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756

Fathead minnow (Pimephales promelas) - MHSF

NOEC SURVIVAL

808

Fathead minnow (Pimephales promelas) - MHSF

IC25** (ON) GROWTH

810

Fathead minnow (Pimephales promelas) - MHSF

NOEC (ON) GROWTH

Test Code 16 (refer to EPA Method 1000.0)

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759

Fathead minnow (Pimephales promelas) - 20% DMW

NOEC SURVIVAL

812

Fathead minnow (Pimephales promelas) - 20% DMW

IC25** (ON) GROWTH

814

Fathead minnow (Pimephales promelas) - 20% DMW

NOEC (ON) GROWTH

Test Code 19 (refer to EPA Method 2002.0)
764

Ceriodaphnia dubia - MHSF 25°C

Test Code 20 (refer to EPA Method 2002.0)
765

Ceriodaphnia dubia - 20% DMW 25°C

Test Code 21 (refer to EPA Method 1002.0)

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LC50

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LC50

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766

Ceriodaphnia dubia – MHSF

NOEC SURVIVAL

767

Ceriodaphnia dubia – MHSF

IC25** REPRODUCTION

768

Ceriodaphnia dubia – MHSF

NOEC REPRODUCTION

Test Code 22 (refer to EPA Method 1002.0)

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769

Ceriodaphnia dubia - 20% DMW

NOEC SURVIVAL

770

Ceriodaphnia dubia - 20% DMW

IC25** REPRODUCTION

771

Ceriodaphnia dubia - 20% DMW

NOEC REPRODUCTION

Test Code 32 (refer to EPA Method 2021.0)

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788

Daphnia magna - MHSF 25°C

Test Code 38 (refer to EPA Method 2021.0)
794

Daphnia pulex - MHSF 25°C

Test Code 42 (refer to EPA Method 2007.0)
798

Mysid (Americamysis bahia, Mysidopsis bahia) 25°C

Test Code 43 (refer to EPA Method 1007.0)

LC50

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LC50

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LC50

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799

Mysid (Americamysis bahia, Mysidopsis bahia)

NOEC SURVIVAL

816

Mysid (Americamysis bahia, Mysidopsis bahia)

IC25** (ON) GROWTH

818

Mysid (Americamysis bahia, Mysidopsis bahia)

NOEC (ON) GROWTH

Test Code 44 (refer to EPA Method 2006.0)
803

Inland silverside (Menidia beryllina) 25°C

Test Code 45 (refer to EPA Method 1006.0)

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LC50

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824

Inland silverside (Menidia beryllina)

NOEC SURVIVAL

825

Inland silverside (Menidia beryllina)

IC25** (ON) GROWTH

826

Inland silverside (Menidia beryllina)

NOEC (ON) GROWTH

Test Code 46 (refer to EPA Method 2004.0)
804

Sheepshead minnow (Cyprinodon variegatus) 25°C

Test Code 47 (refer to EPA Method 1004.0)

———————
LC50

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805

Sheepshead minnow (Cyprinodon variegatus)

NOEC SURVIVAL

820

Sheepshead minnow (Cyprinodon variegatus)

IC25** (ON) GROWTH

822

Sheepshead minnow (Cyprinodon variegatus)

NOEC (ON) GROWTH

Name
Signature
* See Footnotes 2 through 4 on page 5
** Preferred endpoint for DMR-QA performance test reporting

Page 16

2025

Laboratory’s Graded Result
Test
Not Acceptable
Required Acceptable (Corrective Action
Required)

Analyte
determined by
state-certified
lab*

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Date

Complete a separate checklist for EACH lab.