ST-470-20b Pumpkin/Squash/Gourd - Exhibit C

REPRODUCE LOCALLY. Include form number and date on all reproductions.
Form Approved OMB NO 0581-0055
According to the Paperwork Reduction Act of 1995, an agency may not conduct or sponsor, and a person is not required to respond to a collection of information unless it displays a valid OMB control number. The
valid OMB control number for this information collection is 0581-0055. The time required to complete this information collection is estimated to average 2 hours per response, including the time for reviewing
instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection of information.
The U.S. Department of Agriculture (USDA) prohibits discrimination in all its programs and activities on the basis of race, color, national origin, age, disability, and where applicable, sex, marital status, familial status,
parental status, religion, sexual orientation, genetic information, political beliefs, reprisal, or because all or part of an individual’s income is derived from any public assistance program (Not all prohibited bases apply
to all programs.) Persons with disabilities who require alternative means for communication of program information (Braille, large print, audiotape, etc.) should contact USDA’s TARGET Center at (202) 720-2600
(voice and TDD).
To file a complaint of discrimination, write to USDA, Director, Office of Civil Rights, 1400 Independence Avenue, S.W., Washington, D.C. 20250-9410, or call (800) 795-3272 (voice) or (202) 720-6382 (TDD). USDA is
an equal opportunity provider and employer..

U.S. DEPARTMENT OF AGRICULTURE
AGRICULTURAL MARKETING SERVICE
SCIENCE AND TECHNOLOGY
PLANT VARIETY PROTECTION OFFICE
BELTSVILLE, MD 20705

Exhibit C

OBJECTIVE DESCRIPTION OF VARIETY
Pumpkin/Squash/Gourd (Cucurbita spp. ; non pepo’s)
NAME OF APPLICANT (S)

TEMPORARY OR EXPERIMENTAL DESIGNATION

VARIETY NAME

ADDRESS (Street and No. or RD No., City, State, Zip Code and Country)

FOR OFFICIAL USE ONLY
PVPO NUMBER

PLEASE READ ALL INSTRUCTIONS CAREFULLY:
In the spaces on the left, enter the appropriate numbers that describe the characteristics of the application variety. On the right, enter the appropriate numbers
that describe the characteristics of the most similar comparison variety. Right justify whole numbers by adding leading zeros if necessary. The variety that you
choose for comparison should be the most similar one in terms of species, overall morphology, background and maturity. Please follow the guidelines on page 1
for conducting the trials. The comparison variety should be grown in field trials with the application variety for two independent growing cycles, at one or more
localities, in the region and season of best adaptability. In general, measurements of quantitative traits should be taken on at least 24 randomly selected plants or
plant parts to obtain averages and statistics that describe a typical field of the variety. (Form technical content last updated March 2007.)
General Descriptors:

Comparison Variety Name __________________________

01. Species: _________________________________________
(Scientific name, including botanical authority, is mandatory for acceptance of
the application).

01. Species: __________________________

___

02. Expected primary usage:
1 = Culinary
2 = Ornamental
3 = Both
4 = Other (please describe) ____________________________________________

___

02. Expected primary usage
________________________________

___

03. What parts of the plant provide expected primary usage (above):
1 = Mature fruit
2 = Immature fruit
3 = Flowers
4 = Vegetation
5 = Seeds

___

03. Part of plant for #02 above

04. Cotyledons measured between full expansion of first and second true leaves:

04. Cotyledons:

__ . __ __

04a. Length to width ratio (example: 0.00)

__ . __ __

____

04b. Apex

1 = Notched

2 = Not notched

___

04b. Apex

____

04c. Veining

1 = Obscure

2 = Obvious

____

04c. Veining

Application Variety

ST-470-20b (2008) designed by the Plant Variety Protection Office

04a. Length to Width ratio

Comparison Variety

Page 1 of 11

Exhibit C (Pumpkin/Squash/Gourd non pepo’s)
Application Variety
Main Stem:

_____

Comparison variety
Main Stem:

05. Main stem green color, when plants have 20 true leaves on the main stem:

05. Main Stem Color:

___

05a. Main color:
1 = Light
2 = Dark near base only
3 = Dark spots at nodes
4 = Dark for nearly the entire length

___

05a. Main color

___

05b. White marks at nodes:
1 = Absent
2 = Present

___

05b. White marks at nodes

___

05c. Yellow marks (associated with precocious yellow gene complex)
at nodes:
1 = Absent
2 = Present

___

05c. Yellow marks at nodes

___

06. Growth habit

___

07. Tendrils

06. Growth habit when plants have 20 true leaves on the main stem:
Bush
1 = True-bush (Gold Nugget, Redondo del Tronco)
2 = Semi-bush (Bush Pink Banana)
Vine
3 = Moderate vine (Butternut)
4 = Rampant vine (Atlantic Giant, Long Island Cheese)

____

07. Tendrils when plants have 20 true leaves on the main stem:
1 = Absent or rudimentary
2 = Present and elongated
th

08. Main stem internode dimensions when observed after the 20 internode
has developed:

08. Internode dimensions

____

08a. Length
th
th
1 = Internode length constant from 5 to 15 internode
th
th
2 = Internode length increases from 5 to 15 internode

___

08a. Length

____

08b. Width
th
th
3 = Internode width constant from 5 to 15 internode
th
th
4 = Internode width decreases from 5 to 15 internode

___

08b. Width

Petioles:

Petioles:
th

09. Petioles derived from main stem when observed after the 20 node
has developed:
th

__ . __ __ 09a. L:W ratio of 10 petiole

th

__ . __ __ 09b. L:W ratio of 15 petiole

__ . __ __ 09a. Length to medial width ratio of 10 petiole (example: 0.00)
__ . __ __ 09b. Length to medial width ratio of 15 petiole (example: 0.00)

Laminae:
____

th

th

Laminae:
th

th

10. Lobing of 10 and 15 laminae on main stem (Figure 1):
0 = Not lobed
1 = Shallowly lobed
2 = Medium lobed
3 = Deeply lobed 4 = Very deeply lobed
th

11. Dimensions of leaf laminae after the 20 internode has developed (length
measured from the point of petiole attachment to the apex of the lamina;
maximal width measured at 90-degree angle to the length of the lamina):

___

10. Lobing

11. Leaf laminae dimensions:

th

__ . __ __ 11a. L:W ratio of 10 true leaf

__ . __ __ 11b. Length to maximal width ratio of 15 true leaf (example: 0.00)

th

__ . __ __ 11b. L:W ratio of 15 true leaf

12. Silver blotching or mottling (genetic, not leaf-silvering disorder) of adaxial
th
surface of laminae after the 20 internode has developed:
1 = Silver blotching completely absent over time (Waltham Butternut, Gold Nugget)
2 = Silver blotching present early in development, then disappearing
3 = Silver blotching over a small amount of the surface
4 = Silver blotching over a moderate amount of the surface
5 = Silver blotching over much of the surface

___

__ . __ __ 11a. Length to maximal width ratio of 10 true leaf (example: 0.00)

____

09. Petiole measurements:

Application Variety
ST-470-20b (2008) designed by the Plant Variety Protection Office

th

th

12. Silver blotching

Comparison Variety
Page 2 of 11

Exhibit C (Pumpkin/Squash/Gourd non pepo’s)
Application Variety

Comparison Variety

Flowers:

Flowers:

___

13. Number of flowers per node:
1 = Averaging clearly less than one
3 = Often more than one

___

13. Number of flowers per node

2 = One (almost always)
4 = Consistently more than one

14. Staminate flower on day of anthesis on main stem between nodes
11 and 20 (Figure 2):

14. Staminate flower measurements:

__ __ __ mm 14a. Length from base of calyx to tip of corolla

__ __ __ mm 14a. Length of petal

__ __ __ mm 14b. Exterior width at top of calyx cup

__ __ __ mm 14b. Width of petal

__ __ __ mm 14c. Pedicel length

__ __ __ mm 14c. Pedicel length

__ __ __ mm 14d. Length of anther column

__ __ __ mm 14d. Length of anther column

___

15. Dominant color of corolla of staminate flower, on day of anthesis:
1 = Orange-yellow
2 = Intense yellow
3 = Light yellow
4 = Nearly white
5 = Other (please describe) ______________________________________________

___

15. Dominant staminate flower color
____________________________________

___

16. Ring at base of interior of staminate corolla:
1 = Absent
2 = Yellow
3 = Green and yellow
4 = Light green
5 = Dark green

___

16. Ring at base of staminate corolla

___

17. Ring at base of interior of pistillate corolla:
1 = Absent
2 = Yellow
3 = Green and yellow
4 = Light green
5 = Dark green

___

17. Ring at base of pistillate corolla

18. Pistillate flower on day of anthesis:

18. Pistillate flower measurements:

__ __ __ mm 18a. Length from base of calyx to tip of corolla

__ __ __ mm 18a. Length of petal

__ __ __ mm 18b. Pedicel length

__ __ __ mm 18b. Pedicel length

19. Ovary color on day prior to anthesis:
1 = Green
2 = Green turning yellow OR Bi-color green and yellow (Gold Nugget)
3 = Yellow (PI 165558, Prizewinner)

___

___

19. Ovary color

Immature Fruit:

Immature Fruit:

___

20. Fruit shape:
1 = Spherical
2 = Globe OR Oblate (round, but wider than long)
(Long Island Cheese, Musquee de Provence)
3 = Oval OR Oblong (round, but longer than wide) (Upper Ground Sweet Potato)
4 = Bell (Waltham Butternut)
5 = Considerably longer than wide (length to maximal width > 2.0:1) (Lunga di Napoli)
6 = Pyriform (Virginia Mammoth, Golden Cushaw)
7 = Hourglass (Hercules, Toonas Makino)
8 = Turban (Turks Turban, Bonnet Rouge)
9 = Turbinate (top-shaped) (White Rind Sugar)
10 = Fusiform (Hubbard)
11 = Drum-shaped (Buttercup)
12 = Other (please, describe) __________________________________________
__________________________________________________________________
__________________________________________________________________

___

21. Immature fruit size (3–5 days past anthesis) (Figure 3):

21. Immature fruit size

__ . __ __ 21a. Length (through the axis) to medial width ratio (example: 0.00)

__ . __ __ 21a. L:W ratio (to medial width)

__ . __ __ 21b. Length (through the axis) to maximal width ratio (example: 0.00)

__ . __ __ 21b. L:W ratio (to maximal width)

Application Variety

ST-470-20b (2008) designed by the Plant Variety Protection Office

20. Fruit Shape

Comparison Variety

Page 3 of 11

Exhibit C (Pumpkin/Squash/Gourd non pepo’s)
Application Variety

Comparison Variety

Immature Fruit (continued):

Immature Fruit (continued):

____

____

22. Immature fruit color (3–5 days past anthesis):

22. Immature fruit color

___

22a. Main color:
1 = Intense green
2 = Light green (Waltham Butternut)
3 = Yellow (Prizewinner)
4 = Bicolor
5 = Striped green
6 = Other (please describe) ____________________________________
__________________________________________________________

___

22a. Main color

___

22b. If striped, the darker stripes are:
1 = Broad and contiguous (Guatemala Blue)
2 = Narrow and not contiguous

___

22b. Description of darker stripes

___

23. Immature fruit flecks

___

24. Immature fruit warting

23. Immature fruit flecks:
1 = Small
2 = Médium

3 = Large (Waltham Butternut)

24. Immature fruit warting:
1 = Absent (Waltham Butternut, Redondo del Tronco)

2 = Present

Mature Fruit:

Mature Fruit:

____

25. Mature fruit surface topography (fill in the blank with the
most appropriate choice) (Figure 4):
Ribbing present (swelling above vascular tracts):
1 = Prominent and along entire length (Luffa acutangula Rocksberry)
2 = Slight, more prominent near peduncle
3 = Slight, near peduncle
Furrowing (angularly depressed above vascular tracts) and/or ridging (angularly raised
between vascular tracts)
4 = Prominent, along nearly entire length (Yokohama, White Rind Sugar,
Long Island Cheese, Musquee de Provence, Rouge Vif d'Etampes,
Atlantc Giant)
5 = Moderate (Upper Ground Sweet Potato, Lumina, Queensland Blue,
Gold Nugget)
Scalloping (roundly lobed between vascular tracts):
6 = Prominent, at equatorial region
7 = Not so prominent, at equatorial region
8 = Prominent, at peduncular region
9 = Not so prominent, at peduncular region
10 = Prominent, at stylar region
11= Not so prominent, at stylar region
Lobing (broadly and roundly protruding between the vascular tracts and
shallowly depressed along the vascular tracts, along nearly the entire
length of the fruit)
12 = Prominent (Yokohama, White Rind Sugar, Long Island Cheese,
Musquee de Provence, Rouge Vif d'Etampes, Atlantc Giant)
13 = Not so prominent (Upper Ground Sweet Potato, Lumina, Crown Prince,
Gold Nugget)
Grooving (very narrow, shallow depressions along vascular tracts and
midway in-between)
14 = Distinct
15 = Not so distinct
Wrinkling (irregular surface)
16 = Distinct
17 = Indistinct
18 = Completely smooth

___

26. Mature fruit dimensions (at least 40 days past anthesis) (Figure 3):

26. Mature fruit dimensions:

__ . __ __ 26a. Length (through the axis) to medial width ratio (Example: 0.00)

__ . __ __ 26a L:W ratio (to medial width)

__ . __ __ 26b. Length (through the axis) to maximal width ratio (Example: 0.00)

__ . __ __ 26b. L:W ratio (to maximal width)

Application Variety

ST-470-20b (2008) designed by the Plant Variety Protection Office

25. Mature fruit topography

Comparison Variety

Page 4 of 11

Exhibit C (Pumpkin/Squash/Gourd non pepo’s)
Application Variety

Comparison Variety

Mature Fruit (continued):

Mature Fruit (continued):

___

27. Mature fruit warting:
1 = Absent (Waltham Butternut, Gold Nugget)
2 = Sparse, small (Galeux des Antilles) 3 = Sparse, large (Toonas Makino)
4 = Many, small (Essex Hybrid)
5 = Many, large (Marina di Chioggia)

___

27. Mature fruit warting

___

28. Mature fruit rind:
1 = Lignified (when cutting mature fruit, little cracks form) (Gold Nugget)
2 = Not lignified (when cutting mature fruit, they slice smoothly and easily)
(Waltham Butternut)

___

28. Mature fruit rind lignified

___

29. Mature fruit stylar scar:
1 = Protruding
2 = Flat

___

29. Mature fruit stylar scar

3 = Depressed

___

30. Mature fruit stylar end:
1 = Depressed (Prizewinner)
2 = Nearly Flat
3 = Convex (Bush Pink Banana, Gill's Blue Hubbard, Delicious)

___

30. Mature fruit stylar end

___

31. Mature fruit turban:
1 = Absent (Waltham Butternut)
2 = Present
3 = Small (Buttercup)
4 = Large (Turk's Turban)
Colors: ________________________

___

31. Mature fruit turban

___

32. Mature fruit peduncle end:
1 = Depressed
2 = Nearly flat

___

32. Mature fruit peduncle end

3 = Convex

33. Mature fruit peduncle (Figure 5):

33. Mature fruit peduncle dimensions:

__ . __ __ 33a. Length (through the axis) to medial width ratio (Example: 0.00)

__ . __ __ 33a. L:W ratio (to medial width)

__ . __ __ 33b. Length (through the axis) to maximal width (near fruit attachment) ratio
(Example: 0.00)

__ . __ __ 33b. L:W ratio (to maximal width)

34. Mature fruit surface pattern (choose all that apply):
1 = Netted (Golden Cushaw)
2 = Corky (Galeuse d'Eysines)
3 = Cracked (Japanese Pie)
4 = Rough (Valencia)
5 = None of above (please describe) _______________________________________

___

35. Mature fruit exterior color:
35a. Main color (please describe) : ________________________________
____________________________________________________
____________________________________________________
Color Chart Name ______________________
Color Chart Value ______________________

Mature fruit exterior color:
35a. Main color _________________________________
______________________________________________
______________________________________________
Color Chart Name ______________________
Color Chart Value ______________________

35b. Complex colors (give combination of color, with color covering most of the fruit
surface first) ____________________________________________________
__________________________________________________________
__________________________________________________________
Color Chart Name ______________________
Color Chart Value ______________________

35b. Complex colors: _____________________________
______________________________________________
______________________________________________
______________________________________________
Color Chart Name ______________________
Color Chart Value ______________________

___

36. Mature fruit mesocarp (flesh) color:
1 = Intense Orange
2 = Light Orange
3 = Intense Yellow
4 = Light Yellow
5 = Brown
6 = Green
7 = White tinged green
8 = White
9 = Other (describe) __________________________________________
__________________________________________________

___

36. Mature fruit flesh color

___

37. Mature fruit endocarp (placenta) color:
1 = Orange
2 = Yellow
3 = Brown
4 = Green
5 = White
6 = Other (please describe) _____________________________
___________________________________________________

___

37. Mature fruit placenta color

___

Application Variety

ST-470-20b (2008) designed by the Plant Variety Protection Office

34. Mature fruit surface pattern

Comparison Variety

Page 5 of 11

Exhibit C (Pumpkin/Squash/Gourd non pepo’s)
Application Variety

Comparison Variety

Seed:

Seed:

___

____

____

38. Seed cavity:

38. Seed cavity measurements:

__ . __ __ 38a. Length (through the axis) to medial width ratio (Example: 0.00)

__ . __ __ 38a. L:W ratio (to medial width)

__ . __ __ 38b. Length (through the axis) to maximal width ratio (Example: 0.00)

__ . __ __ 38b. L:W ratio (to maximal width)

39. Seed hull (from mature fruit harvested on candidate variety):
1 = Absent
2 = Present but rudimentary 3 = Present with normal appearance

___

40. Seed-coat color (from mature fruit harvested on candidate variety):
Please, describe: _________________________________________________
________________________________________________________________
________________________________________________________________

40. Seed coat color: _____________________________
______________________________________________
______________________________________________

41. Seed dimensions (average for 12 mature seeds from open-pollinated fruit
harvested on candidate variety):

41. Seed measurements

__ . __ __ 41a. Length to width ratio (Example: 0.00)

__ . __ __ 41a. L:W ratio

__ . __ __ 41b. Length to thickness ratio (Example: 0.00)

__ . __ __ 41b. L:Thickness ratio

__ . __ __ 41c. Width to thickness ratio (Example: 0.00)

__ . __ __ 41c. W:Thickness ratio

42. Resistance to biotic or abiotic stresses:
1 = None
2 = Yes, as qualified In Exhibit B or D (specify disease resistance/tolerance):
__________________________________________________________
__________________________________________________________
__________________________________________________________
__________________________________________________________
__________________________________________________________
__________________________________________________________

___

43. Unique features that are not listed in the current 'Exhibit C' and/or are
strongly environmentally dependent or occur sporadically (i.e.: peduncle
characteristics, immature or mature fruit length or contents, width, or weight,
stylar scar size, pollen color, seed-coat characteristics, branching, etc.):
1 = None
2 = Yes, as described herein: __________________________________________
______________________________________________________________
______________________________________________________________
______________________________________________________________
______________________________________________________________
______________________________________________________________

___

39. Seed hull

42. Resistance to biotic or abiotic stresses

_______________________________________
_______________________________________
_______________________________________
_______________________________________
________________________________________
________________________________________

43. Unique features not listed elsewhere in the
application

________________________________________
________________________________________
________________________________________
________________________________________
________________________________________

44. On additional pages, attach photographs of mature fruits of both the application variety and the comparison variety, showing external
and internal coloring, with a ruler in the photograph to indicate scale.
Additional photographs of the plant, flowers, immature fruits, or other plant parts could also be helpful in providing a full description of the
variety to readers. Please provide such photographs if you believe they would be helpful.

References:
Andres, T.C. 2007. The cucurbit network. www.cucurbit.org.
Goldman, A. 2004. The compleat squash. Artisan, New York
Jeffrey, C. 2001. Cucurbitaceae. In: Hanelt, P. et al. Mansfeld's Encyclopedia of agricultural and horticultural crops. Berlin: Springer, 1510-1557.
Missouri Botanical Garden. 2007. Plant Science. Tropical Botanical Science Database. Http://mobot.mobot.org/W3T/Search/vast.html
Paris, H.S. and H. Nerson. 2003. Seed dimensions in the subspecies and cultivar-groups of Cucurbita pepo., Genet. Resources Crop Evol. 50: 615–625.
Robinson, R.W. and D.S. Decker-Walters. 1997. Cucurbits. CAB International, Wallingford, Oxon, UK.

ST-470-20b (2008) designed by the Plant Variety Protection Office

Page 6 of 11

Exhibit C (Pumpkin/Squash/Gourd non pepo’s)

Figure 1. Leaf lobing

ST-470-20b (2008) designed by the Plant Variety Protection Office

Page 7 of 11

Exhibit C (Pumpkin/Squash/Gourd non pepo’s)

Figure 2. Flower measurements

Figure 3. Fruit measurements

ST-470-20b (2008) designed by the Plant Variety Protection Office

Page 8 of 11

Exhibit C (Pumpkin/Squash/Gourd non pepo’s)

Figure 4. Fruit cross-sections

ST-470-20b (2008) designed by the Plant Variety Protection Office

Page 9 of 11

Exhibit C (Pumpkin/Squash/Gourd non pepo’s)

Figure 5. Peduncle measurements

ST-470-20b (2008) designed by the Plant Variety Protection Office

Page 10 of 11

Exhibit C (Pumpkin/Squash/Gourd non pepo’s)

INSTRUCTIONS FOR OBJECTIVE DESCRIPTION OF VARIETY
Pumpkin, Squash, Gourd of all species EXCEPT Cucurbita pepo L.
1. Subject & Purpose of these Guidelines
These Guidelines for testing apply to all varieties of pumpkins, squash, and gourds except for those
belonging to the species Cucurbita pepo L. Their purpose is to tabulate many characteristics in order to
establish the distinguishing phenotypic features of various cultivars of this species.
2. Material Required
a. The applicant, upon receiving a PVP application number and seed-depository letter from the PVP
Examiner, will deposit 3000 (three thousand) seeds at the institution indicated on the depository form.
b. The seed sample should meet normal commercial requirements for germination, which should be stated
by the applicant.
c. The sample must not have undergone any treatment unless the competent authorities allow or request
such treatment. If the seed sample has been treated, full details of the treatment must be given.
3. Conduct of Testing
a. The minimum duration of the test of the variety shall be two independent growing cycles and the test may
be done at one or more localities.
b. The test should be conducted under conditions ensuring satisfactory growth of the plants and normal
expression of the characteristics of the variety under examination.
c. The size of the plots must be large enough to allow the plants to realize their potential. The plots also
must be large enough to allow removal of plants or parts of plants for measurement or counting, if
necessary, without jeopardizing later observations, such as those to be made at the end of the growing
cycle. Each characteristic for testing should be based on a total of at least 24 plants (12 per growing
cycle). Separate plots for observations and for measurements can be used but only if they have been
subjected to similar growing and environmental conditions.
d. Testing for special purposes (disease resistance, vitamin content, etc.) may be established.
4. Methods and Observations
a. All observations determined by measurement or counting should be made on at least 12 plants or parts
taken from each of 12 plants.
b. For the assessment of uniformity, a population standard of 3% should be applied. Where the test is
conducted on 24 plants, the maximum number of off-types allowed would be 2.
5. Grouping of Varieties
The applicant should correctly classify the variety to species together with citation of the botanical authority
(for example: Cucurbita moschata Duchesne). The applicant should suggest, upon submitting the variety for
testing, the market type to which the variety belongs and suggest control varieties of the same species and
type.

ST-470-20b (2008) designed by the Plant Variety Protection Office

Page 11 of 11