LOI2-BIO-19 - Integration of salivary analytes into the NCS: Evaluation of Feasibility, Efficiency, and Benefits

Attachment S3 Goal II Saliva Collection Instructions OMB #: 0925-0647

Expiration Date: 01/31/2015

SALIVA COLLECTION INSTRUCTIONS - MOTHER

Step 1: Record time			Step 2: Select tube
	Record time on sample collection sheet			Match tube color with label on collection sheet
Step 3: Mother saliva collection (straw OR swab method)
		Straw method 1) Insert straw in mouth 2) Allow saliva to collect in tube (1mL) 3) Cap the tube, discard straw			Swab method 1) Insert swab under tongue, close mouth 2) Hold for 2 minutes until saturated 3) Place swab in tube and cap the tube
Step 4: Freeze sample			Good Job!
		Immediately place sample in freezer

SALIVA COLLECTION INSTRUCTIONS – INFANT/CHILD

Step 1: Record time			Step 2: Select tube
	Record time on sample collection sheet			Match tube color with label on collection sheet
Step 4: Infant/Child saliva collection
		1) Firmly grasp one end of swab with gloved hand 2) Place other end of swab under child’s tongue for 2 minutes Do not let go of swab!			3) Switch sides of swab and repeat process 4) Fold swab 5) Place folded swab in tube and cap the tube 6) Discard glove
Step 6: Freeze sample			Good Job!
		Immediately place sample in freezer

Public reporting burden for this collection of information is estimated to average 5 minutes per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection of information. An agency may not conduct or sponsor, and a person is not required to respond to, a collection of information unless it displays a currently valid OMB control number. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden, to: NIH, Project Clearance Branch, 6705 Rockledge Drive, MSC 7974, Bethesda, MD 20892-7974, ATTN: PRA (0925-0647*). Do not return the completed form to this address.