Pre Test

Appendix B 

Test Stimuli  

Attached are the list formats for smokeless tobacco.  The same formats will be 
tested for cigarettes and roll‐your own tobacco. 

U.S. Food and Drug Administration

Logo Sheet 1298.01

Tobacco Product: Brand X Smokeless Tobacco
KEY
Tobacco
Amount Per Gram

Chemicals have been linked to:
CHEMICAL

CA

Acetaldehyde

n

0.1 – 3.5 µg

Arsenic

n

1.1 – 57.3 ng

Benzo[a]pyrene

n

0.1 – 3.1 µg

Cadmium

n

0.5 – 19.4 µg

Crotonaldehyde

n

0.2 -72.3 µg

Formaldehyde

n

0.1 – 1.6 µg

4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)

n

0.97 – 72.3 µg

11.3 – 26.7 mg
0.9 – 6.9 µg

RP

AD
n

n

n
n

n

Nicotine
N-Nitrosonornicotine (NNN)

HB

CA

Cancer

HB

Heart and Blood Vessel Problems

RP

Reproductive Problems

AD

Addiction

ND

Not Detected

n

n

F

The information is not currently
available

There are many other chemicals that have been linked
to the health problems on this list. The information on
these chemicals is not currently available.

A machine is used to test for these chemicals. The amount of chemical that gets into the body may be
higher or lower depending on how a person uses the tobacco product. Companies may use different tests to
measure these chemicals. Results may vary.

mg= milligram
µg = microgram
ng = nanogram
pg= picogram

U.S. Food and Drug Administration

Logo Sheet 1298.01

Tobacco Product: Brand X Smokeless Tobacco
KEY
Tobacco
Amount Per Gram

Chemicals have been linked to:
CHEMICAL

CA

Acetaldehyde

n

0.1 – 3.5 µg

Arsenic

n

1.1 – 57.3 ng

Benzo[a]pyrene

n

0.1 – 3.1 µg

Cadmium

n

0.5 – 19.4 µg

Crotonaldehyde

n

0.2 -72.3 µg

Formaldehyde

n

0.1 – 1.6 µg

4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)

n

0.97 – 72.3 µg

11.3 – 26.7 mg
0.9 – 6.9 µg

HB

AD
n

n

n
n

n

Nicotine
N-Nitrosonornicotine (NNN)

RP

CA

Cancer

HB

Heart and Blood Vessel Problems

RP

Reproductive Problems

AD

Addiction

ND

Not Detected

n

n

The information is not currently available on the following chemicals.

F

The information is not currently
available

Tobacco
Amount Per Gram

CHEMICAL

CA

F

Aflatoxin B1

n

F

Ammonia

F

Anabasine

F

Benz[a]anthracene

n

n

F

Benzo[b]fluoranthene

n

n

F

Benzo[k]fluoranthene

n

n

F

Beryllium

n

F

Chromium

n

F

Chrysene

n

F

Coumarin (banned in food)

F

Dibenz[a,h]anthracene

n

F

Ethyl carbamate (urethane)

n

F

Indeno[1,2,3-cd]pyrene

n

F

Lead

n

F

Mercury

n

F

Naphthalene

n

F

Nickel

n

F

N-Nitrosodiethanolamine (NDELA)

n

F

N-Nitrosodimethylamine (NDMA)

n

F

N-Nitrosomorpholine (NMOR)

n

F

N-Nitrosopiperidine (NPIP)

n

F

N-Nitrosopyrrolidine (NPYR)

n

F

N-Nitrososarcosine (NSAR)

n

F

Nornicotine

F

Polonium-210

F

Selenium

F

Uranium-235

n

F

Uranium-238

n

HB

RP

AD

n

n
n

n
n

n
n

n
n

A machine is used to test for these chemicals. The amount of chemical that gets into the body may be
higher or lower depending on how a person uses the tobacco product. Companies may use different tests to
measure these chemicals. Results may vary.

mg= milligram
µg = microgram
ng = nanogram
pg= picogram

U.S. Food and Drug Administration

Logo Sheet 1298.01

Tobacco Product: Brand X Smokeless Tobacco
KEY
Tobacco
Amount Per Gram

Chemicals have been linked to:
CHEMICAL

CA

Acetaldehyde

n

F

Aflatoxin B1

n

F

Ammonia

F

Anabasine

0.97 – 72.3 µg

HB

n
n

n

F

Benz[a]anthracene

n

n

F

Benzo[b]fluoranthene

n

n

F

Benzo[k]fluoranthene

n

n

Benzo[a]pyrene

n

1.1 – 57.3 ng

n

F

Beryllium

n

0.1 – 3.1 µg

Cadmium

n

n

F

Chromium

n

n

F

Chrysene

n

F

Coumarin (banned in food)
Crotonaldehyde

n

F

Dibenz[a,h]anthracene

n

F

Ethyl carbamate (urethane)

n

Formaldehyde

n

Indeno[1,2,3-cd]pyrene

n

F

Lead

n

F

Mercury

n

4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)

n

F

Naphthalene

n

F

Nickel

n

0.5 – 19.4 µg

0.2 -72.3 µg
F

0.1 – 1.6 µg

11.3 – 26.7 mg

AD
n

Arsenic

0.1 – 3.5 µg

RP

F

N-Nitrosodiethanolamine (NDELA)

n

F

N-Nitrosodimethylamine (NDMA)

n

F

N-Nitrosomorpholine (NMOR)

n

0.9 – 6.9 µg

N-Nitrosonornicotine (NNN)

n

F

N-Nitrosopiperidine (NPIP)

n

F

N-Nitrosopyrrolidine (NPYR)

n

F

N-Nitrososarcosine (NSAR)

n

F

Nornicotine

F

Polonium-210

F

Selenium

F

Uranium-235

n

F

Uranium-238

n

Cancer

HB

Heart and Blood Vessel Problems

RP

Reproductive Problems

AD

Addiction

ND

Not Detected

F

The information is not currently
available

n

mg= milligram
µg = microgram
ng = nanogram
pg= picogram

n

n

n
n

n

Nicotine

CA

n

n
n

A machine is used to test for these chemicals. The amount of chemical that gets into the body may be
higher or lower depending on how a person uses the tobacco product. Companies may use different tests to
measure these chemicals. Results may vary.

U.S. Food and Drug Administration

Logo Sheet 1298.01

Tobacco Product: Brand X Smokeless Tobacco
All tobacco products contain chemicals.

KEY

The purpose of this list is to provide information about the chemicals in this tobacco product

Chemicals have been linked to:

that researchers have linked to health problems. Research is on going to find out which chemicals
in tobacco and tobacco smoke cause harm.

Cancer
Heart and Blood Vessel Problems

There may be other health problems and chemicals that have not been discovered yet.
Tobacco companies test their tobacco for these chemicals and report the amounts to the FDA.

Reproductive Problems

Please note: There is no safe tobacco product. Based on what we currently know, you can not

Addiction

tell your chance of developing a health problem by the number of chemicals or the amount of a
chemical in a tobacco product.

ND
F

TOBACCO

Not Detected
The information is not currently
available

Amount
Per Gram
per gram

CHEMICAL

0.97 – 72.3 µg

Acetaldehyde

n

0.1 – 3.5 µg
SMOKE
1.1 – 57.3 ng

Arsenic

n

Benzo[a]pyrene

n

0.1 – 3.1 µg

Cadmium

n

0.5 – 19.4 µg

Crotonaldehyde

n

0.2 -72.3 µg

Formaldehyde

n

4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)

n

per
cigarette

0.1 – 1.6 µg
P Lmg
ANT
11.3 – 26.7
FOUND IN

0.9 – 6.9 µg

amount per
gram of
tobacco

Nicotine

&

N-Nitrosonornicotine (NNN)
amount per
cigarette

n

TOBACCO

n
n

Where do these chemicals
come from?
per gram

SMOKE

n

FOUND IN

SMOKE

n

n

n

Many of these chemicals come from
the tobacco leaf. The rest come
from additives and the pouch if one is
present.
per
cigarette

FOUND IN

PLANT

amount per
gram of
tobacco

There are many other chemicals that have been linked
to the health problems on this list. The information on
these chemicals is not currently available.

A machine is used to test for these chemicals. The amount of chemical that gets into the body may be
higher or lower depending on how a person uses the tobacco product. Companies may use different tests to
measure these chemicals. Results may vary.

mg= milligram
µg = microgram
ng = nanogram
pg= picogram

&

FOUND IN

SMOKE

amount per
cigarette

U.S. Food and Drug Administration

Logo Sheet 1298.01

Tobacco Product: Brand X Smokeless Tobacco
All tobacco products contain chemicals.

KEY

The purpose of this list is to provide information about the chemicals in this tobacco product

Chemicals have been linked to:

that researchers have linked to health problems. Research is on going to find out which chemicals
in tobacco and tobacco smoke cause harm.

Cancer
Heart and Blood Vessel Problems

There may be other health problems and chemicals that have not been discovered yet.
Tobacco companies test their tobacco for these chemicals and report the amounts to the FDA.

Reproductive Problems

Please note: There is no safe tobacco product. Based on what we currently know, you can not

Addiction

tell your chance of developing a health problem by the number of chemicals or the amount of a
chemical in a tobacco product.

ND
F

TOBACCO

Not Detected
The information is not currently
available

Amount
Per Gram
per gram

CHEMICAL

0.97 – 72.3 µg

Acetaldehyde

n

0.1 – 3.5 µg
SMOKE
1.1 – 57.3 ng

Arsenic

n

Benzo[a]pyrene

n

0.1 – 3.1 µg

Cadmium

n

0.5 – 19.4 µg

Crotonaldehyde

n

0.2 -72.3 µg

Formaldehyde

n

4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)

n

per
cigarette

0.1 – 1.6 µg
P Lmg
ANT
11.3 – 26.7
FOUND IN

0.9 – 6.9 µg

amount per
gram of
tobacco

Nicotine

&

n
n

n

N-Nitrosonornicotine (NNN)
amount per
cigarette

Where do these chemicals
come from?
per gram

SMOKE

n

FOUND IN

SMOKE

TOBACCO

n

n

Many of these chemicals come from
the tobacco leaf. The rest come
from additives and the pouch if one is
present.
per
cigarette

n

The information is not currently available on the following chemicals.

FOUND IN

PLANT

amount per
gram of
tobacco

Amount Per Gram

CHEMICAL

F

Aflatoxin B1

F

Ammonia

F

Anabasine

F

Benz[a]anthracene

n

n

F

Benzo[b]fluoranthene

n

n

F

Benzo[k]fluoranthene

n

n

F

Beryllium

n

F

Chromium

n

F

Chrysene

n

F

Coumarin (banned in food)

F

Dibenz[a,h]anthracene

n

F

Ethyl carbamate (urethane)

n

F

Indeno[1,2,3-cd]pyrene

n

F

Lead

n

F

Mercury

n

F

Naphthalene

n

F

Nickel

n

F

N-Nitrosodiethanolamine (NDELA)

n

F

N-Nitrosodimethylamine (NDMA)

n

F

N-Nitrosomorpholine (NMOR)

n

F

N-Nitrosopiperidine (NPIP)

n

F

N-Nitrosopyrrolidine (NPYR)

n

F

N-Nitrososarcosine (NSAR)

n

F

Nornicotine

F

Polonium-210

F

Selenium

F

Uranium-235

n

F

Uranium-238

n

n
n

n
n

n
n

n
n

n
n

A machine is used to test for these chemicals. The amount of chemical that gets into the body may be
higher or lower depending on how a person uses the tobacco product. Companies may use different tests to
measure these chemicals. Results may vary.

mg= milligram
µg = microgram
ng = nanogram
pg= picogram

&

FOUND IN

SMOKE

amount per
cigarette

U.S. Food and Drug Administration

Logo Sheet 1298.01

Tobacco Product: Brand X Smokeless Tobacco
All tobacco products contain chemicals.

KEY

The purpose of this list is to provide information about the chemicals in this tobacco product

Chemicals have been linked to:

that researchers have linked to health problems. Research is on going to find out which chemicals
in tobacco and tobacco smoke cause harm.

Cancer
Heart and Blood Vessel Problems

There may be other health problems and chemicals that have not been discovered yet.
Tobacco companies test their tobacco for these chemicals and report the amounts to the FDA.

Reproductive Problems

Please note: There is no safe tobacco product. Based on what we currently know, you can not

Addiction

tell your chance of developing a health problem by the number of chemicals or the amount of a
chemical in a tobacco product.

ND
F

TOBACCO

Not Detected
The information is not currently
available

Amount
Per Gram
per gram

CHEMICAL

0.97 – 72.3 µg

Acetaldehyde

n

F
SMOKE
F

Aflatoxin B1

n

F

Anabasine

n

Ammonia
n

Arsenic

n

n

Benz[a]anthracene

n

n

Benzo[b]fluoranthene

n

n

Benzo[k]fluoranthene
SMOKE
per
Benzo[a]pyrene amount
cigarette

n

n

F

Beryllium

n

0.1 – 3.1 µg

Cadmium

n

n

F

Chromium

n

n

F

Chrysene

n

F

Coumarin (banned in food)

0.1 – 3.5 µg
F

per
cigarette

F
FP L A N T
FOUND IN

1.1 – 57.3 ng

amount per
gram of
tobacco

&

FOUND IN

n

F

Dibenz[a,h]anthracene

n

F

Ethyl carbamate (urethane)

n

Formaldehyde

n

F

Indeno[1,2,3-cd]pyrene

n

F

Lead

n

F

Mercury

n

4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)

n

F

Naphthalene

n

F

Nickel

n

0.2 -72.3 µg

0.1 – 1.6 µg

11.3 – 26.7 mg

n

F

N-Nitrosodiethanolamine (NDELA)

n

F

N-Nitrosodimethylamine (NDMA)

n

F

N-Nitrosomorpholine (NMOR)

n

0.9 – 6.9 µg

N-Nitrosonornicotine (NNN)

n

F

N-Nitrosopiperidine (NPIP)

n

F

N-Nitrosopyrrolidine (NPYR)

n

F

N-Nitrososarcosine (NSAR)

n

F

Nornicotine

F

Polonium-210

F

Selenium

F

Uranium-235

n

F

Uranium-238

n

per gram

SMOKE

per
cigarette

FOUND IN

PLANT

amount per
gram of
tobacco

mg= milligram
µg = microgram
ng = nanogram
pg= picogram

n

n

n

n
n

n

Nicotine

Where do these chemicals
come from?
Many of these chemicals come from
the tobacco leaf. The rest come
from additives and the pouch if one is
present.

n

Crotonaldehyde

0.5 – 19.4 µg

TOBACCO

n

n
n

A machine is used to test for these chemicals. The amount of chemical that gets into the body may be
higher or lower depending on how a person uses the tobacco product. Companies may use different tests to
measure these chemicals. Results may vary.

&

FOUND IN

SMOKE

amount per
cigarette