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DIAGNOSTIC PARASITOLOGY: BLOODBORNE
AND INTESTINAL PARASITES
DIVISION OF PARASITIC DISEASES AND MALARIA
CENTERS FOR DISEASE CONTROL AND PREVENTION
SEPTEMBER 24–28, 2018 • ATLANTA, GA
P.A.C.E. ® Course #: 288‐016‐18
Sponsored by:
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Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control
and Prevention, CDC
Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging
and Zoonotic Infectious Diseases, CDC
Division of Laboratory Systems, Center for Surveillance, Epidemiology, and Laboratory Services,
CDC
Location
Centers for Disease Control and Prevention, Atlanta, GA
Faculty
Parasitic Diseases Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, CDC,
Atlanta, GA
Henry Bishop, Microbiologist
Richard Bradbury, PhD, Biologist
MacKevin Ndubuisi, PhD, Biologist
Mark Fox, MS, Fellow
Course Organizers
Division of Laboratory Systems, Center for Surveillance, Epidemiology, and Laboratory Services, CDC
Rebecca Bandea, MS, Health Scientist, E‐mail: [email protected]
Karen Ching, PhD, Health Scientist, E‐mail: [email protected]
Course Objectives
At the conclusion of this program, the participant will be able to:
Explain how to prepare and stain thick and thin blood smears.
Review the morphologic characteristics of Plasmodium spp., Babesia spp., Microfilariae,
Leishmania spp., and Trypanosoma spp.
Detect and identify Plasmodium spp., Babesia spp., Microfilariae, Leishmania spp., and
Trypanosome spp. in clinical specimens.
Discuss rapid diagnostic tests available for identifying Plasmodium spp., Babesia spp.,
Leishmania spp., and Trypanosoma spp.
Review the morphologic characteristics of intestinal helminths and protozoa of public health
concern.
Microscopically detect and identify helminths and protozoa.
Detect Cyclospora cayetanensis using fluorescence microscopy.
Describe the process for clearing or staining proglottids with lactophenol cotton blue or India
ink.
Description
Diagnosis of most parasitic infections is based upon the morphologic characteristics of the different
stages of parasites in and out of their human hosts. Therefore, it is necessary to correctly process,
examine, detect, and identify parasitic organisms from clinical specimens. During this five‐day
intermediate‐level hands‐on workshop, faculty from the Centers for Disease Control and Prevention will
instruct participants on how to detect and identify blood and tissue organisms as well as medically
important intestinal helminths and protozoa.
APPLICATION & REGISTRATION
* FREE REGISTRATION
Application Deadline: July 11, 2018
NEW TWO‐PART APPLICATION PROCESS!
Both parts must be submitted by July 11, 2018 to be considered.
1. Complete the application form online by July 11, 2018.
2. Submit a brief CV or resume highlighting your experience in the area of laboratory testing
relevant to this course by July 11, 2018. Email CV or resume to [email protected]. Type “288‐016‐
18 Diagnostic Parasitology: Bloodborne and Intestinal Parasites” in the subject line of the email.
If you are unable to complete the application online, notify Karen Ching at 404‐498‐6403 or email
[email protected].
Click this link for an example of a brief CV.
Participants will be selected according to the applicants’ job description, experience, and
responsibilities.
Notification of acceptance status will be sent via email by July 17, 2018.
Audience
This intermediate‐level, hands‐on program is intended for laboratorians who work in public health or
clinical microbiology laboratories, are proficient using a microscope, and have experience identifying
intestinal parasites.
Security Clearance Requirements
NON‐US CITIZENS —This course will be held at the training laboratory on the CDC Roybal campus. Due
to CDC requirements for security clearance, all non‐US citizens will be asked to provide information
needed to obtain clearance. Detailed instructions will be provided upon acceptance into the course.
Please do not make any nonrefundable travel plans until you have received confirmation of acceptance
into the course and security clearance approval. The information you provide will only be used for the
purposes of attending this course.
US CITIZENS—If you are a US citizen, there is no extra clearance process required.
Continuing Education Credits
The Centers for Disease Control and Prevention, Division of Laboratory Systems is approved as a
provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E.®
Program. This course is approved for 33.5 contact hours.
Disclosure
CDC, our planners, and our presenters wish to disclose they have no financial interests or other
relationships with the manufacturers of commercial products, suppliers of commercial services, or
commercial supporters. Presentations will not include any discussion of the unlabeled use of a product
or a product under investigational use.
Use of trade names and commercial sources is for identification only and does not imply endorsement
by the Division of Laboratory Systems, Center of Surveillance, Epidemiology, and Laboratory Services,
Centers for Disease Control and Prevention, or the U.S. Department of Health and Human Services.
Special Needs
In compliance with the Americans with Disabilities Act (ADA), individuals seeking special
accommodations should submit their request in writing to [email protected] or phone 404‐639‐4554 at
least three weeks prior to the start date of the course. This will allow sufficient time for CDC to make the
necessary arrangements
QUESTIONS
Please contact Karen Ching at 404‐498‐6403 or email [email protected].
AGENDA
DAY 1—Monday, Sept. 24, 2018
TIME
TYPE
ITEM
SPEAKER
8:00 am
Lecture
Introduction
Karen Ching
8:15
Lecture
Safety Briefing
Becky Bandea
8:30
Lab
Pre‐Test
Henry Bishop
9:30
Break
Break
9:45
Lecture
Plasmodium spp – Overview
Henry Bishop
10:45
Lab
11:30
Lunch
Smear preparation
DPDx Staff
12:30 pm Lecture
Plasmodium falciparum
Henry Bishop
1:00
Lecture
Plasmodium vivax
Henry Bishop
1:30
Lecture
Plasmodium ovale
MacKevin Ndubuisi
2:00
Lecture
MacKevin Ndubuisi
2:15
Break
Plasmodium malariae
2:30
Lab
Plasmodium spp (smears)
DPDx Staff
4:00
Lecture
RDT’s for malaria + Q & A
DPDx Staff
4:30
Adjourn
DPDx Staff
DAY 2—Tuesday, Sept. 25, 2018
TIME
TYPE
ITEM
SPEAKER
8:00 am
Lecture
Babesia
Henry Bishop
8:30
Lab
10:00
Break
Babesia (smears)
DPDx Staff
10:15
Lecture
11:30
Lunch
Microfilariae
Richard Bradbury
Microfilariae (smears)
DPDx Staff
All organisms to date + Q & A
DPDx Staff
12:30 pm Lab
2:45
Break
3:00
Lab
4:30
Adjourn
DAY 3— Wednesday, Sept. 26, 2018
TIME
TYPE
ITEM
SPEAKER
8:00 am
Lecture
Trypanosomes
Richard Bradbury
8:30
Lecture
Leishmania
Richard Bradbury
9:15
Lab
DPDx Staff
10:00
Break
Trypanosomes/Leishmania (smears) + all
organisms to date
10:30
Lab
Post‐test
Henry Bishop
11:30
Lecture
12:00
Lunch
Post‐test evaluation/discussion
DPDx Staff
1:00 pm
Lab
Pre‐test
Henry Bishop
2:00
Lecture
2:45
Break
Flagellates, Ciliates, Blastocystis
DPDx Staff
3:00
Lecture
Intestinal Amoebae
DPDx Staff
3:30
Lab
Protozoa (slides) to date
DPDx Staff
4:30
Adjourn
DAY 4—Thursday, Sept. 27, 2018
TIME
TYPE
ITEM
SPEAKER
8:00 am
Lecture
Coccidia
DPDx Staff
8:30
Lecture
9:15
Break
Microsporidia
Richard Bradbury
9:30
Lab
11:30
Lunch
Protozoa and microsporidia (slides)
DPDx Staff
12:30 pm Lecture
Nematodes
DPDx Staff
1:15
Lab
Nematodes (slides)
DPDx Staff
2:15
Lecture
3:00
Break
Cestodes
DPDx Staff
3:15
Lab
Cestodes (slides)
DPDx Staff
4:30
Adjourn
DAY 5—Friday, Sept. 28, 2018
TIME
TYPE
ITEM
SPEAKER
8:00 am
Lecture
Trematodes
DPDx Staff
8:30
Lab
9:15
Break
Trematodes (slides)
DPDx Staff
9:30
Lab
DPDx Staff
11:30
Lunch
Review all intestinal organisms to date
(slides)
Helminth Procedures (lacto‐phenol
clearing); Review
Henry Bishop
12:30 pm Lab
1:45
Break
2:00
Lab
Post‐test
Henry Bishop
3:00
Lecture
Post‐test evaluation; Q & A
DPDx Staff
3:30
Lecture
Post‐course evaluation; Comments,
critique
Karen Ching
4:30
Adjourn
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