Mycobacterium tuberculosis: Diagnostic Principles and Procedures - Course Screenshot

WORKSHOP
Mycobacterium tuberculosis: Diagnostic Principles and Procedures
March 19-22, 2019
Centers for Disease Control | Atlanta, GA
Co-Sponsored by the Association of Public Health Laboratories in collaboration with the National Center for HIV/
AIDS, Viral Hepatitis, STD, and TB Prevention, Division of Tuberculosis Elimination, and Laboratory Training Team,
Division of Laboratory Systems, Center for Surveillance, Epidemiology, and Laboratory Services, Centers for Disease
Control and Prevention

Course # 588-100-19

DESCRIPTION

APPLICATION to ATTEND

This intermediate-level course will further educate participants
on diagnostic Mycobacterium tuberculosis complex (MTBC)
principles and procedures. Lectures, hands-on laboratory
exercises, group discussions, and interactive sessions will be
used to increase knowledge. State-of-the-art diagnostic
molecular and growth-based methods for detection, isolation,
identification (ID), and drug susceptibility testing (DST) of MTBC
will be discussed and compared. Attendees will be provided
with the tools necessary to determine appropriate safety
practices and testing algorithms. Case studies will highlight
interesting tuberculosis case results including the importance
of accurate result interpretation, collaboration with TB Control
Programs and other laboratories, lessons learned, and problemsolving. Attendees are expected to present case studies and to
participate in group discussions by describing testing
algorithms/methods.

Application Deadline: November 30, 2018
 The preliminary application is to be completed online at

AUDIENCE
This course is intended for laboratorians with a minimum of one
year experience in a laboratory that identifies MTBC and
performs detection, isolation, ID, and DST with preference given
to laboratorians working in public health laboratories.

OBJECTIVES
At the conclusion of this program, the participants will be able
to:
 Identify important risk assessment and biosafety practices
for the mycobacteriology laboratory.
 Compare and contrast test methods for growth-based DST,
ID, and molecular detection of MTBC.
 Describe mutations associated with drug resistance for
MTBC and common correlations between mutations and
growth-based results.
 Perform and interpret real-time PCR for the detection of
MTBC.
 Discuss mycobacteriology case studies related to testing
algorithms and interpretation of results.
 Explain the importance of assessing local data and quality
performance measures for the mycobacteriology
laboratory.
 Recognize accepted validation methods and regulatory
standards for mycobacteriology testing.
 Describe next generation sequencing and its potential use
in the mycobacteriology laboratory.

CONTINUING EDUCATION
The Association of Public Health Laboratories (APHL) is
approved as a provider of continuing education programs in the
clinical laboratory sciences by the ASCLS P.A.C.E.® Program.
Participants who successfully complete this program will be
awarded 24 contact hours. This course has been approved for
24 contact hours in the category (Microbiology/Mycology/
Parasitology) for Florida Laboratory Licensees.
For a complete list of APHL courses, visit www.LaboratoryTraining.org

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https://www.surveymonkey.com/r/100-19TBApplication.
Only completed applications received by the deadline will be
considered. Application does not guarantee acceptance.
If you are unable to complete the application online, email
Marisa Barley at [email protected] or phone
240.485.3843.
Public health applicants must have approval from their state
or local laboratory director to apply. Students will be selected
according to the degree to which the applicant’s job
description, experience, and responsibilities are consistent
with the prerequisites. Priority will be given for one applicant
per public health laboratory, with a second person considered
on a space available basis.
Notification of acceptance status will be sent via email after
December 14, 2018.
Participants are required to bring a case study and testing
algorithm from their laboratory to the course to present to
course participants. More details to follow with application
acceptance.

REGISTRATION
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Registration for this workshop is being offered at No Charge
to the participants!
Registration and logistical details will be provided upon
acceptance into the course.

TRAVEL
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All travel and logistical details will be provided upon
acceptance into the workshop.
Some states have lengthy travel approval processes so it is
important to begin the process as soon as possible. However,
DO NOT make any travel arrangements until you are notified
of acceptance into the course.
Participants are responsible for all lodging, meals, and travel
costs.
A group lodging discount is being negotiated at the current
federal per diem rate of $152.00 (plus tax and fees) per
night.
Transportation between the hotel and CDC will be provided.

SPECIAL NEEDS
In compliance with the Americans with Disabilities Act (ADA),
individuals seeking special accommodations should submit
their request in writing to Marisa Barley, APHL Customer
Support at [email protected], at least three weeks prior to
the start date of the workshop.
This training was supported by Cooperative Agreement #5NU60HM000803-03 funded by the Centers for
Disease Control and Prevention. Its contents are solely the responsibility of the authors and do not
necessarily represent the official views of CDC or the Department of Health and Human Services. This
project was 100% funded with federal funds.

The Association of Public Health Laboratories (APHL)
sponsors educational programs on critical issues in
laboratory science.

Mycobacterium tuberculosis: Diagnostic Principles and Procedures
March 19-22, 2019
Centers for Disease Control | Atlanta, GA
PRELIMINARY AGENDA
Day 1

Tuesday, March 19, 2019

8:00 am
8:30
8:40
9:00
9:30
10:15
10:30

3:45
4:00
4:10
4:40

Introduction and Course Overview
Safety Briefing
Pre-Test
Tuberculosis Epidemiology in the United States
Method Validation and Regulatory Issues
Break
Split Session A
Group 1: Safe Biosafety Cabinet Practices
Group 2: Safety in the TB Laboratory & Risk Assessment
Lunch (on your own)
Split Session B
Group 1: Safety in the TB Laboratory & Risk Assessment
Group 2: Safe Biosafety Cabinet Practices
Considerations for Specimen Processing and Isolation
of MTBC from Culture
Break
Making the Pieces Fit: Combining Growth-Based and
New Mycobacteriology Methods Using a Systems
Approach
DST Reference Center
Results of Demographic Survey
Case Studies
Adjourn

Day 2

Wednesday, March 20, 2019

8:00 am
8:30
9:30
9:45
11:00
11:30
1:00 pm
1:30
2:15
2:30
3:15
4:30

NAAT for Direct Detection of TB
Mycobacterial Identification
Break
Considerations for Growth-based Drug Susceptibility
Testing
Evaluation of MGIT Pyrazinamide Testing
Lunch (on your own)
Monitoring the Performance of Your Laboratory
False-Positive and False-Negative Results
Break
Assessing Local Data
Case Studies
Adjourn

Day 3

Thursday, March 21, 2019

8:00 am
8:30

Introduction to Molecular Biology
Detection of M. tuberculosis complex & M. avium
complex by real-time PCR
Break
Molecular Detection of Drug Resistance
Split Session A
Group 1: Use of real-time PCR Assay for Detection of
MTBC
Group 2: Group Scenarios
Lunch (on your own)
Split Session B
Group 1: Group Scenarios
Group 2: Use of real-time PCR Assay for Detection of
MTBC
Use of Whole Genome Sequencing in Molecular
Epidemiology
Case Studies
Break
Interpretation of real-time PCR Assay Results &
Algorithm Speed Dating
Adjourn

11:30
12:45 pm
1:45
2:45
3:00

9:15
9:30
10:15

11:15
12:45 pm

1:45
2:30
3:00
3:15
4:45

For a complete list of APHL courses, visit www.LaboratoryTraining.org

FACULTY
Division of Tuberculosis Elimination (DTBE), National Center for HIV/
AIDS, Viral Hepatitis, STD, and TB Prevention (NCHHSTP), CDC,
Atlanta, GA
Laboratory Branch, Reference Laboratory Team
 Beverly Metchock, DrPH, D(ABMM), Team Lead
 Kate Klein, MPH, M(ASCP)CM, Microbiologist
 David Sikes, BS, MT(ASCP), Microbiologist
Laboratory Branch, Laboratory Capacity Team
 Stephanie Johnston, MS, Team Lead
 Robert Domaoal, PhD, Laboratory Consultant
 Cortney Stafford, MPH, MT(ASCP), Laboratory Consultant
 Mitchell Yakrus, MS, MPH, Microbiologist
 Monica Youngblood, MPH, M(ASCP), Laboratory Consultant
Laboratory Branch, Applied Research Team
 Jamie Posey, PhD, Team Lead
 Glenn Morlock, MS, Microbiologist
 Melisa Willby, PhD, Microbiologist
Laboratory Branch, Systems Group
 Melinda Dunn, PhD, Safety Officer
Surveillance, Epidemiology, and Outbreak Investigations Branch
 Adam Langer, BS, DVM, MPH, Surveillance Team Lead
Field Services Branch
 Sapna Bamrah Morris, MD, MBA, Medical Officer
Office of Laboratory Safety, Office of the Associate Director for
Laboratory Science and Safety (OADLSS), CDC, Atlanta, GA
 Dwayne Lasky, Safety and Occupational Health Manager

INVITED FACULTY
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Eileen M. Burd, PhD, D(ABMM), Director, Clinical Microbiology,
Emory University Hospital, Atlanta, GA
Jessica Gentry, BA, Supervisor, TB/Serology Laboratory, Indiana
State Department of Health Laboratory
Tanya A. Halse, BS, Supervisor, Molecular Mycobacteriology and
Meningitis Laboratories, New York State Department of Health,
Wadsworth Center
Ryan Jepson, M(ASCP), Supervisor, Microbiology, State Hygienic
Laboratory at the University of Iowa
Jan Owen, BS, TB Reference Team Lead, Texas Department of
State Health Services

Day 4

Friday, March 22, 2019

8:00 am
8:40
9:00
9:15
9:30

Case Studies
Post-Test
Review Test Responses
Break
Beyond the Laboratory Walls: Enhancing Your Integrated
System
Case Studies: Big Picture
Evaluation
Final Question and Answer
Adjourn

10:15
11:45
12:00 pm
12:15

NOTE: CDC SECURITY CLEARANCE REQUIREMENTS
NON-US CITIZENS - This course will be held at the CDC Roybal campus. Due to CDC
requirements for security clearance, all non-US citizens will be asked to provide
information needed to obtain clearance, which will only be used for the purposes of
attending this course. Detailed instructions will be provided upon acceptance into the
course. Please do not make any nonrefundable travel plans until you have received
confirmation of acceptance into the course and security clearance approval.
US CITIZENS - If you are a US CITIZEN there is no extra
clearance process required.

The Association of Public Health Laboratories (APHL)
sponsors educational programs on critical issues in
laboratory science.