Document
Component 1 - STLT Health Depts
ICR 202609-0920-006 · OMB 0920-1422 · Object 173173700.
Document Viewer [xlsx]
Status: The document is viewable through fallback artifacts while conversion repair continues in the background.
Queue: A repair attempt failed. The document remains flagged for retry and verification. (priority 90 · attempts 10 · next 2026-10-08 03:50:08)
Missing: html
Download: Source copy (xlsx) | pdf
Loading document viewer…
Document Metadata
| File Type | application/vnd.openxmlformats-officedocument.spreadsheetml.sheet |
|---|---|
| File Title | Component 1 - STLT Health Depts |
| Author | SRA |
| Last Modified By | Calc |
| File Modified | 2025-08-29 |
| File Created | 2026-10-07 |
| Conversion State | failed_conversion |
Extracted Text
Instructions: Each column that has a red triangle in the upper-right corner has a comment that can be displayed if yo Some column headers have hyperlinks to NCBI webpages. The YELLOW columns have drop-down menues that allow you to select from a controlled vocabulary. O You must save spreadsheet under second tab (SRA_data) as a TSV (tab-delimited file) to uploa If you created samples previously, provide accessions in the form of SAMN# in the column sample_accession. Otherwise provide the sample name used in the BioSample attributes spreadsheet. Each row in the template represents a sequencing library with a unique combination of sample + library + sequencing strategy + layout + instrument model. Each row should have a unique library_id that is short and meaningful (like an ID you might use in lab). When libraries are indeed identical (same combination of sample + library + strategy + layout + instrument model), all files should be placed in the same row To do this simply enter the file names consecutively in the same row by adding more columns with headers filename2, filename3, etc…. PAIRED files must always be listed in the same row. Provide exact file names (including extensions) in the filename columns. File names must be unique. red triangles indicate pop-up comments for that field Many of the columns also have data checks - if you received a warning, please verify that you have ente NOTE: There are data checks and autocomplete features in this spreadsheet that are not compatible wi manually consult the platform and instrument information on the last page. Example Drop Down Menu Helpful Hyperlinks: SRA submission overview: SRA submission in Submission Portal: You must save spreadsheet under second tab (SRA_data) as a TSV (tab-delimited file) to uploa er has a comment that can be displayed if you hover over the header. you to select from a controlled vocabulary. Once specified for one row, these values can be copied-and-pasted down. ata) as a TSV (tab-delimited file) to upload the TSV file for the SRA metadata tab. required for ALL data types required for aligned data paired-end data only d a warning, please verify that you have entered a value from the drop-down menu. Example Below. this spreadsheet that are not compatible with Libre- and Open-Office. If you use one of these suites, please on the last page. http://www.ncbi.nlm.nih.gov/sra/docs/submit/ http://www.ncbi.nlm.nih.gov/sra/docs/submitportal/ ata) as a TSV (tab-delimited file) to upload the TSV file for the SRA metadata tab. opied-and-pasted down. suites, please sample_name library_ID biosample_id nwss_sample_idX title library_strategy Targeted Amplification sequencing of wastewater sample library_source cing of wastewater sample library_selection library_layout platform ILLUMINA instrument_model design_description filetype Illumina iSeq 100 methods included in custom attributes filename filename2 filename3 filename4 assembly fasta_fileenrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_methodsequence_submitter_contact_email raw_sequence_data_processing_method Required? Column Name required for ALL data types sample_name required for ALL data types library_ID required for ALL data types title required for ALL data types library_strategy required for ALL data types library_source required for ALL data types library_selection required for ALL data types library_layout required for ALL data types platform required for ALL data types instrument_model required for ALL data types design_description required for ALL data types filetype required for ALL data types filename filename2 filename3 filename4 assembly fasta_file required for ALL data types enrichment_kit required for ALL data types amplicon_PCR_primer_scheme required for ALL data types library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details required for ALL data types dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_methrequired for ALL data types Comments Targeted Amplification sequencing of waste Required for aligned data Required for aligned data Enter the name of the PCR kit used for amp Select the primers or kit used for amplicon g Enter the name of the library preparation ki Would it work if both method and version w Is version required? Can be intentionally left blank Can be left blank if no issues pop up Any additional details one wishes to include Recommended to use NCBI human-read scr Contact e-mail is optional If none, note "no further processing" sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method sample_name library_ID title library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 filename3 filename4 assembly fasta_file enrichment_kit amplicon_PCR_primer_scheme library_preparation_kit amplicon_size quality_control_method quality_control_method_version quality_control_determination quality_control_issues quality_control_details dehosting_method sequence_submitter_contact_email raw_sequence_data_processing_method on sequencing of wastewater sample he PCR kit used for amplification r kit used for amplicon generation he library preparation kit used for sequencing h method and version were in 1 cell? o issues pop up s one wishes to include about QC goes here se NCBI human-read scrubber. Note if other methods are used instead ther processing" Strategy WCS Random sequencing of the whole genome following non-pcr amplification Random sequencing of exonic the whole genome regions selected from the genome Random sequencing of whole transcriptome Random sequencing of small miRNAs Random sequencing of a whole chromosome or other replicon isolated from a genome CLONE Genomic clone based (hierarchical) sequencing POOLCLONE Shotgun of pooled clones (usually BACs and Fosmids) AMPLICON Sequencing of overlapping or distinct PCR or RT-PCR products Clone end (5', 3', or both) sequencing Sequencing intended to finish (close) gaps in existing coverage WGA WGS WXS RNA-Seq miRNA-Seq CLONEEND FINISHING ChIP-Seq MNase-Seq Direct sequencing of chromatin immunoprecipitates Direct sequencing following MNase digestion Tn-Seq EST FL-cDNA CTS Sequencing of hypersensitive sites, or segments of open chromatin that are more readily cleaved by DNaseI Sequencing following treatment of DNA with bisulfite to convert cytosine residues to uracil depending on methylation status Sequencing from transposon insertion sites Single pass sequencing of cDNA templates Full-length sequencing of cDNA templates Concatenated Tag Sequencing MRE-Seq Methylation-Sensitive Restriction Enzyme Sequencing strategy MeDIP-Seq Methylated DNA Immunoprecipitation Sequencing strategy Direct sequencing of methylated fractions sequencing strategy DNase-Hypersensitivity Bisulfite-Seq MBD-Seq Synthetic-Long-Read ATAC-seq Assay for Transposase-Accessible Chromatin (ATAC) strategy is used to study ChIA-PET Direct sequencing of proximity-ligated chromatin immunoprecipitates. FAIRE-seq Formaldehyde Assisted Isolation of Regulatory Elements. reveals regions of o Hi-C Chromosome Conformation Capture technique where a biotin-labeled nucle ncRNA-Seq Capture of other non-coding RNA types, including post-translation modificat RAD-Seq RIP-Seq Direct sequencing of RNA immunoprecipitates (includes CLIP-Seq, HITS-CLIP SELEX Systematic Evolution of Ligands by EXponential enrichment ssRNA-seq strand-specific RNA sequencing Targeted-Capture Tethered Chromatin Conformation Capture OTHER Source GENOMIC TRANSCRIPTOMIC METAGENOMIC METATRANSCRIPTOMIC SYNTHETIC VIRAL RNA GENOMIC SINGLE CELL TRANSCRIPTOMIC SINGLE CELL Library strategy not listed (please include additional info in the “design description”) Genomic DNA (includes PCR products from genomic DNA) Transcription products or non genomic DNA (EST, cDNA, RTPCR, screened libraries) Mixed material from metagenome Transcription products from community targets Synthetic DNA Viral RNA OTHER Other, unspecified, or unknown library source material (please include additional info in the “design description”) Selection RANDOM PCR RANDOM PCR RT-PCR HMPR MF CF-S Random selection by shearing or other method Source material was selected by designed primers Source material was selected by randomly generated primers Source material was selected by reverse transcription PCR Hypo-methylated partial restriction digest Methyl Filtrated Cot-filtered single/low-copy genomic DNA CF-M CF-H CF-T Cot-filtered moderately repetitive genomic DNA Cot-filtered highly repetitive genomic DNA Cot-filtered theoretical single-copy genomic DNA Multiple displacement amplification MDA Methylation Spanning Linking Library MSLL complementary DNA cDNA Chromatin immunoprecipitation ChIP Micrococcal Nuclease (MNase) digestion MNase Deoxyribonuclease (MNase) digestion DNAse Selection by hybridization in array or solution Hybrid Selection Reproducible genomic subsets, often generated by restriction fragment size selection, containing a manageable number of Reduced Representation loci to facilitate re-sampling DNA fractionation using restriction enzymes Restriction Digest Selection of methylated DNA fragments using an antibody 5-methylcytidine antibody raised against 5-methylcytosine or 5-methylcytidine (m5C) MBD2 protein methyl-CpG binding domain Enrichment by methyl-CpG binding domain Cap-analysis gene expression CAGE Rapid Amplification of cDNA Ends RACE Physical selection of size appropriate targets size fractionation Circularized oligonucleotide probes Padlock probes capture method other unspecified Other library enrichment, screening, or selection process (please include additional info in the “design description”) Library enrichment, screening, or selection is not specified (please include additional info in the “design description”) cDNA_oligo_dT cDNA_randomPriming Inverse rRNA depletion of ribosomal RNA by oligo hybridization. Oligo-dT enrichment of messenger RNA (mRNA) by hybridization to Oligo-dT. PolyA repeat fractionation PolyA selection or enrichment for messenger RNA (mRNA); should replace cD Selection for less repetitive (and more gene rich) sequence through Cot filtra Platforms _LS454 ABI_SOLID BGISEQ CAPILLARY COMPLETE_GENOMICS HELICOS ILLUMINA ION_TORRENT OXFORD_NANOPORE PACBIO_SMRT _LS454 454 GS 454 GS 20 454 GS FLX 454 GS FLX+ 454 GS FLX Titanium 454 GS Junior ILLUMINA HiSeq X Five HiSeq X Ten Illumina Genome Analyzer Illumina Genome Analyzer II Illumina Genome Analyzer IIx Illumina HiScanSQ Illumina HiSeq 1000 Illumina HiSeq 1500 Illumina HiSeq 2000 Illumina HiSeq 2500 Illumina HiSeq 3000 Illumina HiSeq 4000 Illumina iSeq 100 Illumina NovaSeq 6000 Illumina MiniSeq Illumina MiSeq NextSeq 500 NextSeq 550 NextSeq 1000 NextSeq 2000 Illumina HiSeq X strategy is used to study genome-wide chromatin accessibility. alternative method to DNase-seq that uses an engineered Tn5 trans mmunoprecipitates. ents. reveals regions of open chromatin re a biotin-labeled nucleotide is incorporated at the ligation junction, enabling selective purification of chimeric DNA ligation junctio ost-translation modification types such as snRNA (small nuclear RNA) or snoRNA (small nucleolar RNA), or expression regulation typ udes CLIP-Seq, HITS-CLIP and PAR-CLIP). tion to Oligo-dT. mRNA); should replace cDNA enumeration. quence through Cot filtration (CF) or other fractionation techniques based on DNA kinetics. HELICOS Helicos HeliScope ABI_SOLID COMPLETE_GENOMICS PACBIO_SMRT PacBio RS AB 5500 Genetic AnalyzeComplete Genomics PacBio RS II AB 5500xl Genetic Analyzer AB 5500x-Wl Genetic Analyzer PacBio Sequel AB SOLiD 3 Plus System PacBio Sequel II AB SOLiD 4 System AB SOLiD 4hq System AB SOLiD PI System AB SOLiD System AB SOLiD System 2.0 AB SOLiD System 3.0 eq that uses an engineered Tn5 transposase to cleave DNA and to integrate primer DNA sequences into the cleaved genomic DNA ation of chimeric DNA ligation junctions followed by deep sequencing ar RNA), or expression regulation types such as siRNA (small interfering RNA) or piRNA/piwi/RNA (piwi-interacting RNA). ION_TORRENT Ion Torrent PGM Ion Torrent Proton Ion Torrent S5 XL Ion Torrent S5 CAPILLARY AB 310 Genetic Analyzer AB 3130 Genetic Analyzer AB 3130xL Genetic Analyzer AB 3500 Genetic Analyzer AB 3500xL Genetic Analyzer AB 3730 Genetic Analyzer AB 3730xL Genetic Analyzer OXFORD_NANOPORE GridION MinION PromethION BGISEQ BGISEQ-500 DNBSEQ-G400 DNBSEQ-T7 DNBSEQ-G50 MGISEQ-2000RS into the cleaved genomic DNA wi-interacting RNA). enrichment_kit amplicon_PCR_primer_scheme ARTIC v5.4.2 ARTIC v5.3.2 ARTIC v5.3 ARTIC v5.2.0 ARTIC v5.1.0 ARTIC v5.0.0 ARTIC v4.1 ARTIC v4 ARTIC v3 ARTIC v2 ARTIC v1 NEB VarSkip Short / VarSkip Short v1a NEB VarSkip Short v2 / VarSkip v2a NEB VarSkip Short v2b NEB VarSkip / VarSkip Long / VarSkip Long 1a Midnight Midnight v2 Midnight v3 AmpliSeq SARS-CoV-2 Research Panel (Illumina) ARTIC SARS-CoV-2 Amplicon Panel (IDT) CleanPlex SARS-CoV-2 FLEX Kit (Paragon Genomics) CleanPlex SARS-CoV-2 Kit (Paragon Genomics) CleanPlex SARS-CoV-2 Emerging Variants Panel Add-on v2 (Paragon Genomics) CleanPlex Respiratory Virus Research Panel v2 (Paragon Genomics) Clear Dx FlexPro: Wastewater (SARS-CoV-2) (Clear Labs) Clear Dx SARS-CoV-2 WGS v2.0 (Clear Labs) Clear Dx SARS-CoV-2 WGS v3.0 (Clear Labs) COVID Mini v14: 576 samples (ONT) COVIDSeq Assay (96 samples) (Illumina) COVIDSeq Test (RUO Version) (Illumina) COVIDSeq v3 Primer Pool (Illumina) COVIDSeq v4 Primer Pool (Illumina) EasySeq SARS-CoV-2 WGS Library Prep Kit v4 (NimaGen) EasySeq SARS-CoV-2 WGS Library Prep Kit v5 (NimaGen) HIFIVIRAL SARS-COV-2 (PacBio) Ion AmpliSeq SARS-CoV-2 sequencing (Thermo Fisher) Midnight RT PCR Expansion (ONT) NEBNext ARTIC SARS-CoV-2 Companion Kit - ARTIC v3 (NEB) NEBNext ARTIC SARS-CoV-2 Companion Kit - VarSkip Short v2 (NEB) NEBNext ARTIC SARS-CoV-2 FS Library Prep Kit - ARTIC v3 (NEB) NEBNext ARTIC SARS-CoV-2 FS Library Prep Kit - VarSkip Short v2 (NEB) NEBNext ARTIC SARS-CoV-2 Library Prep Kit - ARTIC v3 (NEB) NEBNext ARTIC SARS-CoV-2 RT-PCR Module - ARTIC v3 (NEB) NEBNext ARTIC SARS-CoV-2 RT-PCR Module - VarSkip Short v2 (NEB) QIAseq DIRECT SARS-CoV-2 (Qiagen) QIAseq DIRECT SARS-CoV-2 Boosted (Qiagen) SNAP - Swift Normalase Amplicon SARS-COV-2 Additional Genome Coverage Panel (Swift Biosciences) SNAP - Swift Normalase Amplicon SARS-COV-2 Panel (Swift Biosciences) SNAP - Swift Normalase Amplicon SARS-COV-2 S Gene Panel (Swift Biosciences) xGen SARS-CoV-2 Amplicon Panel (IDT) xGen SARS-CoV-2 Midnight Amplicon Panel (IDT) xGen SARS-CoV-2 S-gene Amplicon Panel (IDT) amplicon_PCR_primer_scheme_no_longer_included Cotten Eden Gonzalez-Reiche Itokawa Resende SARS-CoV-2 SNAP primer pool UNZA Sanger Midnight 1200/Freed primers library_preparation_kit Covidmini kit Illumina DNA prep Illumina Nextera XT Illumina TrueSeq Illumina RNA Prep with Enrichment, (L) Tagmentation Illumina COVIDSeq Assay Ligation sequencing kit Midnight expansion kit NEBNext ARTIC SARS-CoV-2 Library Prep Kit (Illumina) NEBNext Ultra II FS DNA Library Prep for Illumina QIAseq DIRECT Unique Dual Index prep Rapid barcoding kit gon Genomics) dehosting_method ncov-dehoster Nanostripper 0.1alpha SRA human read removal tool me Coverage Panel (Swift Biosciences) ft Biosciences) quality_control_method_name GalaxyTrakr SSQuAWK CFSAN Wastewater Analysis Pipeline (C-WAP) quality_control_method_version quality_control_determination no quality control issues identified minor quality control issues identified sequence flagged for potential quality control issues not performed quality_control_issues low quality sequence sequenced contaminated low average genome coverage low % genome captured read lengths shorter than expected sequence amplification artifacts low signal to noise ratio low coverage of characteristic mutations quality_control_details stic mutations Update from v5.7 to v5.8 Date Updated: 2025-04-21 1. The "enrichment_kit" field is now a free-text field, allowing users to provide the name of the PCR kit used for amp 2. The "amplicon_PCR_primer_scheme" field has been updated: outdated primer scheme options were removed, an ame of the PCR kit used for amplification instead of selecting from a predefined drop-down list. heme options were removed, and the list was expanded to include primers and kits used for amplicon generation. plicon generation.